Effect of recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge with space/providing biomaterials on the augmentation of chronic alveolar ridge defects

Eliane Porto Barboza, André Luis Caúla, Fernanda de Oliveira Caúla, Rogério Oliveira de Souza, Luiz Geolás Neto, Rachel G. Sorensen, X. Jian Li, Ulf M E Wikesjö

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier has been shown to support significant bone formation in the craniofacial skeleton. When used as an onlay, however, rhBMP-2/ACS may become compressed with limited resulting bone formation. The objective of this study was to evaluate the effect of two space-providing biomaterials, bioactive glass (BG) and demineralized/mineralized bone matrix (DMB), on rhBmp-2/ACS induced alveolar ridge augmentation. Methods: Bilateral alveolar ridge defects were produced in the mandible in six mongrel dogs. rhBMP-2/ACS with biomaterials was surgically implanted into contralateral defects in four animals. Treatments were alternated between jaw quadrants in consecutive animals. Two animals received rhBMP-2/ACS or sham-surgery in contralateral defects. The animals were injected with fluorescent bone labels to monitor bone formation. Clinical evaluations were made at ridge augmentation and 12 weeks post-implantation when the animals were euthanized and block biopsies collected for histopathologic evaluation. Results: Sham-surgery produced limited horizontal alveolar augmentation (0.1 ± 0.6 mm). Implantation of rhBMP-2/ACS resulted in alveolar augmentation amounting to 2.2 ± 1.8 mm. Alveolar augmentation in sites receiving rhBMP-2/ACS with DMB or BG was 2-fold greater compared to rhBMP-2/ACS alone averaging 4.4 ± 1.3 and 4.6 ± 1.5 mm, respectively. The DMB biomaterial appeared substituted by newly formed bone. The BG particles were observed imbedded in bone or encapsulated in dense connective tissue without associated bone metabolic activity. Fluorescent light microscopy suggested that the new bone was formed within 4 weeks. Conclusion: The bioglass and demineralized/mineralized bone matrix biomaterials utilized in this study in combination with rhBMP-2/ACS supported clinical and histological ridge augmentation.

Original languageEnglish (US)
Pages (from-to)702-708
Number of pages7
JournalJournal of periodontology
Volume75
Issue number5
StatePublished - May 1 2004

Fingerprint

Alveolar Ridge Augmentation
Porifera
Biocompatible Materials
Collagen
Bone Matrix
Bone and Bones
Osteogenesis
Glass
recombinant human bone morphogenetic protein-2
Alveolar Process
Inlays
Jaw
Mandible
Skeleton
Connective Tissue
Microscopy

Keywords

  • Alveolar ridge augmentation
  • Animal studies
  • Bone matrix
  • Bone regeneration
  • Glass, biologically active
  • Proteins, bone morphogenetic
  • Proteins, recombinant

ASJC Scopus subject areas

  • Periodontics

Cite this

Porto Barboza, E., Caúla, A. L., de Oliveira Caúla, F., Oliveira de Souza, R., Geolás Neto, L., Sorensen, R. G., ... Wikesjö, U. M. E. (2004). Effect of recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge with space/providing biomaterials on the augmentation of chronic alveolar ridge defects. Journal of periodontology, 75(5), 702-708.

Effect of recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge with space/providing biomaterials on the augmentation of chronic alveolar ridge defects. / Porto Barboza, Eliane; Caúla, André Luis; de Oliveira Caúla, Fernanda; Oliveira de Souza, Rogério; Geolás Neto, Luiz; Sorensen, Rachel G.; Li, X. Jian; Wikesjö, Ulf M E.

In: Journal of periodontology, Vol. 75, No. 5, 01.05.2004, p. 702-708.

Research output: Contribution to journalArticle

Porto Barboza, E, Caúla, AL, de Oliveira Caúla, F, Oliveira de Souza, R, Geolás Neto, L, Sorensen, RG, Li, XJ & Wikesjö, UME 2004, 'Effect of recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge with space/providing biomaterials on the augmentation of chronic alveolar ridge defects', Journal of periodontology, vol. 75, no. 5, pp. 702-708.
Porto Barboza E, Caúla AL, de Oliveira Caúla F, Oliveira de Souza R, Geolás Neto L, Sorensen RG et al. Effect of recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge with space/providing biomaterials on the augmentation of chronic alveolar ridge defects. Journal of periodontology. 2004 May 1;75(5):702-708.
Porto Barboza, Eliane ; Caúla, André Luis ; de Oliveira Caúla, Fernanda ; Oliveira de Souza, Rogério ; Geolás Neto, Luiz ; Sorensen, Rachel G. ; Li, X. Jian ; Wikesjö, Ulf M E. / Effect of recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge with space/providing biomaterials on the augmentation of chronic alveolar ridge defects. In: Journal of periodontology. 2004 ; Vol. 75, No. 5. pp. 702-708.
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abstract = "Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier has been shown to support significant bone formation in the craniofacial skeleton. When used as an onlay, however, rhBMP-2/ACS may become compressed with limited resulting bone formation. The objective of this study was to evaluate the effect of two space-providing biomaterials, bioactive glass (BG) and demineralized/mineralized bone matrix (DMB), on rhBmp-2/ACS induced alveolar ridge augmentation. Methods: Bilateral alveolar ridge defects were produced in the mandible in six mongrel dogs. rhBMP-2/ACS with biomaterials was surgically implanted into contralateral defects in four animals. Treatments were alternated between jaw quadrants in consecutive animals. Two animals received rhBMP-2/ACS or sham-surgery in contralateral defects. The animals were injected with fluorescent bone labels to monitor bone formation. Clinical evaluations were made at ridge augmentation and 12 weeks post-implantation when the animals were euthanized and block biopsies collected for histopathologic evaluation. Results: Sham-surgery produced limited horizontal alveolar augmentation (0.1 ± 0.6 mm). Implantation of rhBMP-2/ACS resulted in alveolar augmentation amounting to 2.2 ± 1.8 mm. Alveolar augmentation in sites receiving rhBMP-2/ACS with DMB or BG was 2-fold greater compared to rhBMP-2/ACS alone averaging 4.4 ± 1.3 and 4.6 ± 1.5 mm, respectively. The DMB biomaterial appeared substituted by newly formed bone. The BG particles were observed imbedded in bone or encapsulated in dense connective tissue without associated bone metabolic activity. Fluorescent light microscopy suggested that the new bone was formed within 4 weeks. Conclusion: The bioglass and demineralized/mineralized bone matrix biomaterials utilized in this study in combination with rhBMP-2/ACS supported clinical and histological ridge augmentation.",
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AU - Porto Barboza, Eliane

AU - Caúla, André Luis

AU - de Oliveira Caúla, Fernanda

AU - Oliveira de Souza, Rogério

AU - Geolás Neto, Luiz

AU - Sorensen, Rachel G.

AU - Li, X. Jian

AU - Wikesjö, Ulf M E

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N2 - Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier has been shown to support significant bone formation in the craniofacial skeleton. When used as an onlay, however, rhBMP-2/ACS may become compressed with limited resulting bone formation. The objective of this study was to evaluate the effect of two space-providing biomaterials, bioactive glass (BG) and demineralized/mineralized bone matrix (DMB), on rhBmp-2/ACS induced alveolar ridge augmentation. Methods: Bilateral alveolar ridge defects were produced in the mandible in six mongrel dogs. rhBMP-2/ACS with biomaterials was surgically implanted into contralateral defects in four animals. Treatments were alternated between jaw quadrants in consecutive animals. Two animals received rhBMP-2/ACS or sham-surgery in contralateral defects. The animals were injected with fluorescent bone labels to monitor bone formation. Clinical evaluations were made at ridge augmentation and 12 weeks post-implantation when the animals were euthanized and block biopsies collected for histopathologic evaluation. Results: Sham-surgery produced limited horizontal alveolar augmentation (0.1 ± 0.6 mm). Implantation of rhBMP-2/ACS resulted in alveolar augmentation amounting to 2.2 ± 1.8 mm. Alveolar augmentation in sites receiving rhBMP-2/ACS with DMB or BG was 2-fold greater compared to rhBMP-2/ACS alone averaging 4.4 ± 1.3 and 4.6 ± 1.5 mm, respectively. The DMB biomaterial appeared substituted by newly formed bone. The BG particles were observed imbedded in bone or encapsulated in dense connective tissue without associated bone metabolic activity. Fluorescent light microscopy suggested that the new bone was formed within 4 weeks. Conclusion: The bioglass and demineralized/mineralized bone matrix biomaterials utilized in this study in combination with rhBMP-2/ACS supported clinical and histological ridge augmentation.

AB - Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier has been shown to support significant bone formation in the craniofacial skeleton. When used as an onlay, however, rhBMP-2/ACS may become compressed with limited resulting bone formation. The objective of this study was to evaluate the effect of two space-providing biomaterials, bioactive glass (BG) and demineralized/mineralized bone matrix (DMB), on rhBmp-2/ACS induced alveolar ridge augmentation. Methods: Bilateral alveolar ridge defects were produced in the mandible in six mongrel dogs. rhBMP-2/ACS with biomaterials was surgically implanted into contralateral defects in four animals. Treatments were alternated between jaw quadrants in consecutive animals. Two animals received rhBMP-2/ACS or sham-surgery in contralateral defects. The animals were injected with fluorescent bone labels to monitor bone formation. Clinical evaluations were made at ridge augmentation and 12 weeks post-implantation when the animals were euthanized and block biopsies collected for histopathologic evaluation. Results: Sham-surgery produced limited horizontal alveolar augmentation (0.1 ± 0.6 mm). Implantation of rhBMP-2/ACS resulted in alveolar augmentation amounting to 2.2 ± 1.8 mm. Alveolar augmentation in sites receiving rhBMP-2/ACS with DMB or BG was 2-fold greater compared to rhBMP-2/ACS alone averaging 4.4 ± 1.3 and 4.6 ± 1.5 mm, respectively. The DMB biomaterial appeared substituted by newly formed bone. The BG particles were observed imbedded in bone or encapsulated in dense connective tissue without associated bone metabolic activity. Fluorescent light microscopy suggested that the new bone was formed within 4 weeks. Conclusion: The bioglass and demineralized/mineralized bone matrix biomaterials utilized in this study in combination with rhBMP-2/ACS supported clinical and histological ridge augmentation.

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KW - Animal studies

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KW - Glass, biologically active

KW - Proteins, bone morphogenetic

KW - Proteins, recombinant

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