Effect of sex steroids and insulin on dehydroepiandrosterone sulfate production by hepatoma G2 cells

Marita Pall, Margaret Nguyen, Denis Magoffin, Ricardo Azziz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To test the hypothesis that DHEAS production from DHEA occurs in hepatic cells and that this production is augmented by the presence of sex steroids or insulin. Design: In vitro prospective experiment. Setting: Academic medical center. Intervention(s): Hepatoma G2 cells cultured in media supplemented with [1] DHEA (10-5 mol/L) only, [2] DHEA (10-5 mol/L) + T (10-6 mol/L), [3] DHEA (10-5 mol/L) + E2 (10-6 mol/L), [4] DHEA (10-5 mol/L) + dihydrotestosterone (10-6 mol/L), [5] DHEA (10-5 mol/L) + insulin (10 ng/mL), or [6] DHEA (10-5 mol/L) + insulin (100 ng/mL). Main Outcome Measure(s): Levels of DHEAS in the media were measured at 0, 2, 4, 6, 8, 12, 24, 48, and 72 hours after adding treatments at time-point 0. Result(s): Dehydroepiandrosterone sulfate was first detected in the hepatoma G2 cell culture media at 12 hours of incubation. The cumulative production rate of DHEAS increased linearly until 72 hours of incubation. When compared with the effect of treatment with DHEA only, treatment with DHEA plus T, dihydrotestosterone, or E2 delayed the cumulative DHEAS production; alternatively, the addition of insulin did not alter DHEAS production. Conclusion(s): These data suggest that although hepatic cells have the ability of converting DHEA to DHEAS, neither sex steroids nor insulin results in the increased hepatic production of DHEAS.

Original languageEnglish (US)
Pages (from-to)2551-2556
Number of pages6
JournalFertility and sterility
Volume91
Issue number6
DOIs
StatePublished - Jun 1 2009

Fingerprint

Dehydroepiandrosterone Sulfate
Dehydroepiandrosterone
Hepatocellular Carcinoma
Steroids
Insulin
Dihydrotestosterone
Hepatocytes
Culture Media
Cultured Cells
Cell Culture Techniques
Outcome Assessment (Health Care)

Keywords

  • Adrenal androgen excess
  • DHEA sulfotransferase
  • DHEAS
  • HepG2 cells
  • SULT2A1
  • dehydroepiandrosterone

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Effect of sex steroids and insulin on dehydroepiandrosterone sulfate production by hepatoma G2 cells. / Pall, Marita; Nguyen, Margaret; Magoffin, Denis; Azziz, Ricardo.

In: Fertility and sterility, Vol. 91, No. 6, 01.06.2009, p. 2551-2556.

Research output: Contribution to journalArticle

Pall, Marita ; Nguyen, Margaret ; Magoffin, Denis ; Azziz, Ricardo. / Effect of sex steroids and insulin on dehydroepiandrosterone sulfate production by hepatoma G2 cells. In: Fertility and sterility. 2009 ; Vol. 91, No. 6. pp. 2551-2556.
@article{ddbf7d703f374493ab9dc61332e61d27,
title = "Effect of sex steroids and insulin on dehydroepiandrosterone sulfate production by hepatoma G2 cells",
abstract = "Objective: To test the hypothesis that DHEAS production from DHEA occurs in hepatic cells and that this production is augmented by the presence of sex steroids or insulin. Design: In vitro prospective experiment. Setting: Academic medical center. Intervention(s): Hepatoma G2 cells cultured in media supplemented with [1] DHEA (10-5 mol/L) only, [2] DHEA (10-5 mol/L) + T (10-6 mol/L), [3] DHEA (10-5 mol/L) + E2 (10-6 mol/L), [4] DHEA (10-5 mol/L) + dihydrotestosterone (10-6 mol/L), [5] DHEA (10-5 mol/L) + insulin (10 ng/mL), or [6] DHEA (10-5 mol/L) + insulin (100 ng/mL). Main Outcome Measure(s): Levels of DHEAS in the media were measured at 0, 2, 4, 6, 8, 12, 24, 48, and 72 hours after adding treatments at time-point 0. Result(s): Dehydroepiandrosterone sulfate was first detected in the hepatoma G2 cell culture media at 12 hours of incubation. The cumulative production rate of DHEAS increased linearly until 72 hours of incubation. When compared with the effect of treatment with DHEA only, treatment with DHEA plus T, dihydrotestosterone, or E2 delayed the cumulative DHEAS production; alternatively, the addition of insulin did not alter DHEAS production. Conclusion(s): These data suggest that although hepatic cells have the ability of converting DHEA to DHEAS, neither sex steroids nor insulin results in the increased hepatic production of DHEAS.",
keywords = "Adrenal androgen excess, DHEA sulfotransferase, DHEAS, HepG2 cells, SULT2A1, dehydroepiandrosterone",
author = "Marita Pall and Margaret Nguyen and Denis Magoffin and Ricardo Azziz",
year = "2009",
month = "6",
day = "1",
doi = "10.1016/j.fertnstert.2008.03.049",
language = "English (US)",
volume = "91",
pages = "2551--2556",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Effect of sex steroids and insulin on dehydroepiandrosterone sulfate production by hepatoma G2 cells

AU - Pall, Marita

AU - Nguyen, Margaret

AU - Magoffin, Denis

AU - Azziz, Ricardo

PY - 2009/6/1

Y1 - 2009/6/1

N2 - Objective: To test the hypothesis that DHEAS production from DHEA occurs in hepatic cells and that this production is augmented by the presence of sex steroids or insulin. Design: In vitro prospective experiment. Setting: Academic medical center. Intervention(s): Hepatoma G2 cells cultured in media supplemented with [1] DHEA (10-5 mol/L) only, [2] DHEA (10-5 mol/L) + T (10-6 mol/L), [3] DHEA (10-5 mol/L) + E2 (10-6 mol/L), [4] DHEA (10-5 mol/L) + dihydrotestosterone (10-6 mol/L), [5] DHEA (10-5 mol/L) + insulin (10 ng/mL), or [6] DHEA (10-5 mol/L) + insulin (100 ng/mL). Main Outcome Measure(s): Levels of DHEAS in the media were measured at 0, 2, 4, 6, 8, 12, 24, 48, and 72 hours after adding treatments at time-point 0. Result(s): Dehydroepiandrosterone sulfate was first detected in the hepatoma G2 cell culture media at 12 hours of incubation. The cumulative production rate of DHEAS increased linearly until 72 hours of incubation. When compared with the effect of treatment with DHEA only, treatment with DHEA plus T, dihydrotestosterone, or E2 delayed the cumulative DHEAS production; alternatively, the addition of insulin did not alter DHEAS production. Conclusion(s): These data suggest that although hepatic cells have the ability of converting DHEA to DHEAS, neither sex steroids nor insulin results in the increased hepatic production of DHEAS.

AB - Objective: To test the hypothesis that DHEAS production from DHEA occurs in hepatic cells and that this production is augmented by the presence of sex steroids or insulin. Design: In vitro prospective experiment. Setting: Academic medical center. Intervention(s): Hepatoma G2 cells cultured in media supplemented with [1] DHEA (10-5 mol/L) only, [2] DHEA (10-5 mol/L) + T (10-6 mol/L), [3] DHEA (10-5 mol/L) + E2 (10-6 mol/L), [4] DHEA (10-5 mol/L) + dihydrotestosterone (10-6 mol/L), [5] DHEA (10-5 mol/L) + insulin (10 ng/mL), or [6] DHEA (10-5 mol/L) + insulin (100 ng/mL). Main Outcome Measure(s): Levels of DHEAS in the media were measured at 0, 2, 4, 6, 8, 12, 24, 48, and 72 hours after adding treatments at time-point 0. Result(s): Dehydroepiandrosterone sulfate was first detected in the hepatoma G2 cell culture media at 12 hours of incubation. The cumulative production rate of DHEAS increased linearly until 72 hours of incubation. When compared with the effect of treatment with DHEA only, treatment with DHEA plus T, dihydrotestosterone, or E2 delayed the cumulative DHEAS production; alternatively, the addition of insulin did not alter DHEAS production. Conclusion(s): These data suggest that although hepatic cells have the ability of converting DHEA to DHEAS, neither sex steroids nor insulin results in the increased hepatic production of DHEAS.

KW - Adrenal androgen excess

KW - DHEA sulfotransferase

KW - DHEAS

KW - HepG2 cells

KW - SULT2A1

KW - dehydroepiandrosterone

UR - http://www.scopus.com/inward/record.url?scp=67349191449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349191449&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2008.03.049

DO - 10.1016/j.fertnstert.2008.03.049

M3 - Article

C2 - 18554595

AN - SCOPUS:67349191449

VL - 91

SP - 2551

EP - 2556

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 6

ER -