Effect of sodium metabisulfite on hydrogen peroxide production in light- exposed pediatric parenteral amino acid solutions

Vince Brawley, Jatinder J Bhatia, Warren B. Karp

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effect of sodium metabisulfite (MBS) on hydrogen peroxide (HP) production in model and commercial amino acid solutions exposed to phototherapy light was studied. Model and commercial pediatric amino acid solutions were prepared such that the amino acid concentration was 1%. MBS concentration, riboflavin concentration, and duration of exposure to phototherapy light were varied to determine the effect on HP production. Control solutions were kept in the dark. HP production was assayed in the model amino acid solutions by using potassium iodide in the presence of ammonium molybdate. In all experiments, HP production was measured at 360 nm in the presence and absence of catalase. In light-exposed solutions, HP production increased linearly for several hours and reached a plateau by eight hours. A mean maximum of 940 μM was produced (data pooled for all solutions). No detectable HP was generated in the solutions kept in the dark. After two hours of light exposure, it was necessary to add at least 10 times more MBS than is typically found in commercial total parenteral nutrient solutions to scavenge all the HP produced. An average of up to 940 μM of HP was produced in model and commercial pediatric parenteral 1% amino acid solutions in the presence of phototherapy light and clinically relevant concentrations of riboflavin and MBS. Light exposure decreased the anti- oxidant effect of MBS.

Original languageEnglish (US)
Pages (from-to)1288-1292
Number of pages5
JournalAmerican Journal of Health-System Pharmacy
Volume55
Issue number12
StatePublished - Jun 15 1998

Fingerprint

Hydrogen Peroxide
Pediatrics
Light
Amino Acids
Phototherapy
Riboflavin
Potassium Iodide
sodium metabisulfite
Catalase
Antioxidants
metabisulfite

Keywords

  • Amino acids
  • Antioxidants
  • Concentration
  • Contamination
  • Hydrogen peroxide
  • Nutrition
  • Pediatrics
  • Photodecomposition
  • Riboflavin
  • Sodium metabisulfite
  • Stability

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Leadership and Management

Cite this

Effect of sodium metabisulfite on hydrogen peroxide production in light- exposed pediatric parenteral amino acid solutions. / Brawley, Vince; Bhatia, Jatinder J; Karp, Warren B.

In: American Journal of Health-System Pharmacy, Vol. 55, No. 12, 15.06.1998, p. 1288-1292.

Research output: Contribution to journalArticle

@article{1268bdb231e9418094c9c3c8cb61c746,
title = "Effect of sodium metabisulfite on hydrogen peroxide production in light- exposed pediatric parenteral amino acid solutions",
abstract = "The effect of sodium metabisulfite (MBS) on hydrogen peroxide (HP) production in model and commercial amino acid solutions exposed to phototherapy light was studied. Model and commercial pediatric amino acid solutions were prepared such that the amino acid concentration was 1{\%}. MBS concentration, riboflavin concentration, and duration of exposure to phototherapy light were varied to determine the effect on HP production. Control solutions were kept in the dark. HP production was assayed in the model amino acid solutions by using potassium iodide in the presence of ammonium molybdate. In all experiments, HP production was measured at 360 nm in the presence and absence of catalase. In light-exposed solutions, HP production increased linearly for several hours and reached a plateau by eight hours. A mean maximum of 940 μM was produced (data pooled for all solutions). No detectable HP was generated in the solutions kept in the dark. After two hours of light exposure, it was necessary to add at least 10 times more MBS than is typically found in commercial total parenteral nutrient solutions to scavenge all the HP produced. An average of up to 940 μM of HP was produced in model and commercial pediatric parenteral 1{\%} amino acid solutions in the presence of phototherapy light and clinically relevant concentrations of riboflavin and MBS. Light exposure decreased the anti- oxidant effect of MBS.",
keywords = "Amino acids, Antioxidants, Concentration, Contamination, Hydrogen peroxide, Nutrition, Pediatrics, Photodecomposition, Riboflavin, Sodium metabisulfite, Stability",
author = "Vince Brawley and Bhatia, {Jatinder J} and Karp, {Warren B.}",
year = "1998",
month = "6",
day = "15",
language = "English (US)",
volume = "55",
pages = "1288--1292",
journal = "American Journal of Health-System Pharmacy",
issn = "1079-2082",
publisher = "American Society of Health-Systems Pharmacy",
number = "12",

}

TY - JOUR

T1 - Effect of sodium metabisulfite on hydrogen peroxide production in light- exposed pediatric parenteral amino acid solutions

AU - Brawley, Vince

AU - Bhatia, Jatinder J

AU - Karp, Warren B.

PY - 1998/6/15

Y1 - 1998/6/15

N2 - The effect of sodium metabisulfite (MBS) on hydrogen peroxide (HP) production in model and commercial amino acid solutions exposed to phototherapy light was studied. Model and commercial pediatric amino acid solutions were prepared such that the amino acid concentration was 1%. MBS concentration, riboflavin concentration, and duration of exposure to phototherapy light were varied to determine the effect on HP production. Control solutions were kept in the dark. HP production was assayed in the model amino acid solutions by using potassium iodide in the presence of ammonium molybdate. In all experiments, HP production was measured at 360 nm in the presence and absence of catalase. In light-exposed solutions, HP production increased linearly for several hours and reached a plateau by eight hours. A mean maximum of 940 μM was produced (data pooled for all solutions). No detectable HP was generated in the solutions kept in the dark. After two hours of light exposure, it was necessary to add at least 10 times more MBS than is typically found in commercial total parenteral nutrient solutions to scavenge all the HP produced. An average of up to 940 μM of HP was produced in model and commercial pediatric parenteral 1% amino acid solutions in the presence of phototherapy light and clinically relevant concentrations of riboflavin and MBS. Light exposure decreased the anti- oxidant effect of MBS.

AB - The effect of sodium metabisulfite (MBS) on hydrogen peroxide (HP) production in model and commercial amino acid solutions exposed to phototherapy light was studied. Model and commercial pediatric amino acid solutions were prepared such that the amino acid concentration was 1%. MBS concentration, riboflavin concentration, and duration of exposure to phototherapy light were varied to determine the effect on HP production. Control solutions were kept in the dark. HP production was assayed in the model amino acid solutions by using potassium iodide in the presence of ammonium molybdate. In all experiments, HP production was measured at 360 nm in the presence and absence of catalase. In light-exposed solutions, HP production increased linearly for several hours and reached a plateau by eight hours. A mean maximum of 940 μM was produced (data pooled for all solutions). No detectable HP was generated in the solutions kept in the dark. After two hours of light exposure, it was necessary to add at least 10 times more MBS than is typically found in commercial total parenteral nutrient solutions to scavenge all the HP produced. An average of up to 940 μM of HP was produced in model and commercial pediatric parenteral 1% amino acid solutions in the presence of phototherapy light and clinically relevant concentrations of riboflavin and MBS. Light exposure decreased the anti- oxidant effect of MBS.

KW - Amino acids

KW - Antioxidants

KW - Concentration

KW - Contamination

KW - Hydrogen peroxide

KW - Nutrition

KW - Pediatrics

KW - Photodecomposition

KW - Riboflavin

KW - Sodium metabisulfite

KW - Stability

UR - http://www.scopus.com/inward/record.url?scp=0032526722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032526722&partnerID=8YFLogxK

M3 - Article

VL - 55

SP - 1288

EP - 1292

JO - American Journal of Health-System Pharmacy

JF - American Journal of Health-System Pharmacy

SN - 1079-2082

IS - 12

ER -