Effect of structural change of collagen fibrils on the durability of dentin bonding

Bin Yang, Rainer Adelung, Klaus Ludwig, Klaus Bößmann, David Henry Pashley, Matthias Kern

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

This study investigates the effect of structural changes of collagen fibrils on the bonding durability of a total etch luting resin (Super-Bond C&B) and a self-etching luting resin (Panavia F 2.0) to dentin. An atomic force microscope (AFM) was used to observe structural changes of intact dentin collagen fibrils after acidic conditionings of two bonding systems. After 90 d water storage and 15,000 thermal cycles (TC) as artificial aging, micro-tensile bond strength (μTBS) was utilized to evaluate the bonding durability of the two bonding systems to dentin. μTBS after 1 d or 90 d water storage without TC were separately measured in control groups. A cross-banding periodicity of about 67 nm along collagen fibrils was seen on demineralized intertubular dentin surfaces in AFM images. For both luting resins, thermal cycling decreased (p<0.05) μTBS of 1 d and 90 d, compared to controls. Scanning electron microscope and transmission electron microscopic examinations revealed that the top and bottom of hybrid layer (HL) were weak links in the bonding interface over time. The results suggest that the top of HL contains disorganized collagen fibrils from the smear layer which degrade over time. AFM results indicate that the demineralized intact collagen fibrils beneath the smear layer were not denatured during acidic conditioning. However, these collagen fibrils may be structurally unstable due to poor infiltration by resin or loss of resin protection within the HL over time, reducing the long-term μTBS. This process was accelerated by thermal fatigue cycling.

Original languageEnglish (US)
Pages (from-to)5021-5031
Number of pages11
JournalBiomaterials
Volume26
Issue number24
DOIs
StatePublished - Aug 1 2005

Fingerprint

Dentin
Collagen
Durability
Tensile Strength
Resins
Hot Temperature
Smear Layer
Microscopes
Electrons
Thermal fatigue
Water
Thermal cycling
Periodicity
Infiltration
Fatigue
Etching
Microscopic examination
Electron microscopes
Aging of materials
Scanning

Keywords

  • AFM
  • Collagen fibrils
  • Dentin bonding
  • Durability
  • SEM
  • TEM

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Yang, B., Adelung, R., Ludwig, K., Bößmann, K., Pashley, D. H., & Kern, M. (2005). Effect of structural change of collagen fibrils on the durability of dentin bonding. Biomaterials, 26(24), 5021-5031. https://doi.org/10.1016/j.biomaterials.2005.01.024

Effect of structural change of collagen fibrils on the durability of dentin bonding. / Yang, Bin; Adelung, Rainer; Ludwig, Klaus; Bößmann, Klaus; Pashley, David Henry; Kern, Matthias.

In: Biomaterials, Vol. 26, No. 24, 01.08.2005, p. 5021-5031.

Research output: Contribution to journalArticle

Yang, B, Adelung, R, Ludwig, K, Bößmann, K, Pashley, DH & Kern, M 2005, 'Effect of structural change of collagen fibrils on the durability of dentin bonding', Biomaterials, vol. 26, no. 24, pp. 5021-5031. https://doi.org/10.1016/j.biomaterials.2005.01.024
Yang, Bin ; Adelung, Rainer ; Ludwig, Klaus ; Bößmann, Klaus ; Pashley, David Henry ; Kern, Matthias. / Effect of structural change of collagen fibrils on the durability of dentin bonding. In: Biomaterials. 2005 ; Vol. 26, No. 24. pp. 5021-5031.
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