Effect of systemic parathyroid hormone (1-34) and a β-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model

Jonathan I. Yun, Ulf Me Wikesjö, James L. Borke, Frederick C. Bisch, Jill E. Lewis, Robert W. Herold, Gary D. Swiec, Joseph C. Wood, James C. McPherson

Research output: Contribution to journalArticle

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Abstract

Objective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1-34) (PTH), a surgically implanted synthetic β-tricalcium phosphate (β-TCP) bone biomaterial serving as a matrix to support new bone formation. Materials and Methods: Critical-size, 8 mm, calvarial through-and-through osteotomy defects were surgically created in 100 adult male Sprague-Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β-TCP, β-TCP, or particulate human demineralized freeze-dried bone (DFDB), and sham-surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post-surgery for radiographic and histometric analysis. Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0% compared with PTH/β-TCP (15.7±2.4%), β-TCP (12.5±2.3%), DFDB (14.5±2.3%), and sham-surgery control (10.0±1.5%) at 4 weeks (p<0.014). Systemic PTH showed significantly enhanced bone formation (41.5±4.0%) compared with PTH/β-TCP (22.4±3.0%), β-TCP (21.3±4.4%), and with the sham-surgery control (23.8±4.2%) at 8 weeks (p<0.025). The DFDB group showed significantly increased bone formation from 4 (14.5±2.3%) to 8 weeks (32.0±3.2%) (p<0.006). The PTH/β-TCP and β-TCP groups both showed limited biomaterials resorption. The radiographic analysis was not diagnostic to distinguish local bone formation from the radiopaque β-TCP biomaterial. Conclusions: Systemic administration of PTH significantly stimulates local bone formation. Bone formation was significantly limited by the β-TCP biomaterial.

Original languageEnglish (US)
Pages (from-to)419-426
Number of pages8
JournalJournal of Clinical Periodontology
Volume37
Issue number5
DOIs
StatePublished - May 1 2010

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Biocompatible Materials
Parathyroid Hormone
Osteogenesis
Bone and Bones
tricalcium phosphate
Osteotomy
Sprague Dawley Rats

Keywords

  • β-tricalcium phosphate
  • Guided bone regeneration
  • Parathyroid hormone
  • Rat calvaria model
  • Tissue engineering

ASJC Scopus subject areas

  • Periodontics

Cite this

Effect of systemic parathyroid hormone (1-34) and a β-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model. / Yun, Jonathan I.; Wikesjö, Ulf Me; Borke, James L.; Bisch, Frederick C.; Lewis, Jill E.; Herold, Robert W.; Swiec, Gary D.; Wood, Joseph C.; McPherson, James C.

In: Journal of Clinical Periodontology, Vol. 37, No. 5, 01.05.2010, p. 419-426.

Research output: Contribution to journalArticle

Yun, Jonathan I. ; Wikesjö, Ulf Me ; Borke, James L. ; Bisch, Frederick C. ; Lewis, Jill E. ; Herold, Robert W. ; Swiec, Gary D. ; Wood, Joseph C. ; McPherson, James C. / Effect of systemic parathyroid hormone (1-34) and a β-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model. In: Journal of Clinical Periodontology. 2010 ; Vol. 37, No. 5. pp. 419-426.
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abstract = "Objective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1-34) (PTH), a surgically implanted synthetic β-tricalcium phosphate (β-TCP) bone biomaterial serving as a matrix to support new bone formation. Materials and Methods: Critical-size, 8 mm, calvarial through-and-through osteotomy defects were surgically created in 100 adult male Sprague-Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β-TCP, β-TCP, or particulate human demineralized freeze-dried bone (DFDB), and sham-surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post-surgery for radiographic and histometric analysis. Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0{\%} compared with PTH/β-TCP (15.7±2.4{\%}), β-TCP (12.5±2.3{\%}), DFDB (14.5±2.3{\%}), and sham-surgery control (10.0±1.5{\%}) at 4 weeks (p<0.014). Systemic PTH showed significantly enhanced bone formation (41.5±4.0{\%}) compared with PTH/β-TCP (22.4±3.0{\%}), β-TCP (21.3±4.4{\%}), and with the sham-surgery control (23.8±4.2{\%}) at 8 weeks (p<0.025). The DFDB group showed significantly increased bone formation from 4 (14.5±2.3{\%}) to 8 weeks (32.0±3.2{\%}) (p<0.006). The PTH/β-TCP and β-TCP groups both showed limited biomaterials resorption. The radiographic analysis was not diagnostic to distinguish local bone formation from the radiopaque β-TCP biomaterial. Conclusions: Systemic administration of PTH significantly stimulates local bone formation. Bone formation was significantly limited by the β-TCP biomaterial.",
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AU - Yun, Jonathan I.

AU - Wikesjö, Ulf Me

AU - Borke, James L.

AU - Bisch, Frederick C.

AU - Lewis, Jill E.

AU - Herold, Robert W.

AU - Swiec, Gary D.

AU - Wood, Joseph C.

AU - McPherson, James C.

PY - 2010/5/1

Y1 - 2010/5/1

N2 - Objective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1-34) (PTH), a surgically implanted synthetic β-tricalcium phosphate (β-TCP) bone biomaterial serving as a matrix to support new bone formation. Materials and Methods: Critical-size, 8 mm, calvarial through-and-through osteotomy defects were surgically created in 100 adult male Sprague-Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β-TCP, β-TCP, or particulate human demineralized freeze-dried bone (DFDB), and sham-surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post-surgery for radiographic and histometric analysis. Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0% compared with PTH/β-TCP (15.7±2.4%), β-TCP (12.5±2.3%), DFDB (14.5±2.3%), and sham-surgery control (10.0±1.5%) at 4 weeks (p<0.014). Systemic PTH showed significantly enhanced bone formation (41.5±4.0%) compared with PTH/β-TCP (22.4±3.0%), β-TCP (21.3±4.4%), and with the sham-surgery control (23.8±4.2%) at 8 weeks (p<0.025). The DFDB group showed significantly increased bone formation from 4 (14.5±2.3%) to 8 weeks (32.0±3.2%) (p<0.006). The PTH/β-TCP and β-TCP groups both showed limited biomaterials resorption. The radiographic analysis was not diagnostic to distinguish local bone formation from the radiopaque β-TCP biomaterial. Conclusions: Systemic administration of PTH significantly stimulates local bone formation. Bone formation was significantly limited by the β-TCP biomaterial.

AB - Objective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1-34) (PTH), a surgically implanted synthetic β-tricalcium phosphate (β-TCP) bone biomaterial serving as a matrix to support new bone formation. Materials and Methods: Critical-size, 8 mm, calvarial through-and-through osteotomy defects were surgically created in 100 adult male Sprague-Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β-TCP, β-TCP, or particulate human demineralized freeze-dried bone (DFDB), and sham-surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post-surgery for radiographic and histometric analysis. Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0% compared with PTH/β-TCP (15.7±2.4%), β-TCP (12.5±2.3%), DFDB (14.5±2.3%), and sham-surgery control (10.0±1.5%) at 4 weeks (p<0.014). Systemic PTH showed significantly enhanced bone formation (41.5±4.0%) compared with PTH/β-TCP (22.4±3.0%), β-TCP (21.3±4.4%), and with the sham-surgery control (23.8±4.2%) at 8 weeks (p<0.025). The DFDB group showed significantly increased bone formation from 4 (14.5±2.3%) to 8 weeks (32.0±3.2%) (p<0.006). The PTH/β-TCP and β-TCP groups both showed limited biomaterials resorption. The radiographic analysis was not diagnostic to distinguish local bone formation from the radiopaque β-TCP biomaterial. Conclusions: Systemic administration of PTH significantly stimulates local bone formation. Bone formation was significantly limited by the β-TCP biomaterial.

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KW - Guided bone regeneration

KW - Parathyroid hormone

KW - Rat calvaria model

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