Effect of test medium on in vitro susceptibility testing results for Aspergillus fumigatus

Elias Kurian Manavathu, U. Nune, K. Kanuri, P. H. Chandrasekar, O. C. Abraham

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We examined the effect of peptone yeast extract glucose broth (PYG), RPMI 1640 and Antibiotic Medium 3 (M3) on the in vitro susceptibility of clinical isolates (n = 200) of Aspergillus fumigatus to amphotericin B (AMB), itraconazole (ITZ) and voriconazole (VCZ). The MICs (μg/mL) of various antifungal agents (geometric mean ± standard deviation) obtained in PYG, RPMI 1640 and M3, respectively, were as follows; AMB: 1.64 ± 0.92, 0.42 ± 0.21, 0.33 ± 0.16; ITZ: 0.44 ± 0.54, 0.40 ± 0.65, 0.17 ± 0.32; VCZ: 0.70 ± 0.58, 0.29 ± 0.22, 0.36 ± 0.21. Pair-wise comparisons of the MICs of AMB, ITZ and VCZ obtained in PYG, RPMI 1640 and M3 showed no significant differences except for AMB in PYG broth compared to those obtained in RPMI 1640 and M3 (p≤0.05). These results suggest that the susceptibility of A. fumigatus to AMB, ITZ and VCZ is similar in growth media currently used for susceptibility testing.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalRevista Iberoamericana de Micologia
Volume17
Issue number3
Publication statusPublished - Jan 1 2000

    Fingerprint

Keywords

  • Antifungal
  • Aspergillus fumigatus
  • Broth microdilution
  • Filamentous fungi
  • Susceptibility testing

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Manavathu, E. K., Nune, U., Kanuri, K., Chandrasekar, P. H., & Abraham, O. C. (2000). Effect of test medium on in vitro susceptibility testing results for Aspergillus fumigatus. Revista Iberoamericana de Micologia, 17(3), 107-110.