Effect of tetracycline HCl on periodontally-affected human root surfaces

L. Trombelli, A. Scabbia, F. Zangari, A. Griselli, U. M E Wikesjo, G. Calura

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Scanning electron microscopy was used to evaluate surface characteristics of periodontitis-exposed instrumented human cementum and dentin surfaces following topical application of tetracycline HCl (TTC). Specimens were randomly assigned to application of sterile saline for 1 minute (control); TTC (10 mg/ml) for 1 minute and 4 minutes, respectively; and TTC (100 mg/ml) for 1 minute and 4 minutes, respectively. Solutions were applied with a cotton pellet using a burnishing technique. Control specimens exhibited an amorphous irregular surface smear layer. TTC treatment of cementum for 1 minute resulted in a relatively debris-free, nonhomogeneous surface. The 4-minute application resulted in a surface exhibiting a densely fibrillar, mat-like texture. Dentin specimens conditioned for 1 minute showed a smooth surface with many tubule openings partially occluded by debris. The 4-minute treatment exposed a 3-dimensional network of intertubular and peritubular collagen fibrils. No consistent morphologic differences were observed between cementum or dentin specimens treated with TTC at concentrations of 10 and 100 mg/ml, respectively. The results suggest that topical application of TTC produces morphologic alterations of periodontitis-exposed cementum and dentin that appear related to application interval rather than concentration of the drug.

Original languageEnglish (US)
Pages (from-to)685-691
Number of pages7
JournalJournal of periodontology
Issue number8
StatePublished - Jan 1 1995


  • cementum/anatomy and histology
  • dentin/anatomy and histology
  • tetracycline/therapeutic use

ASJC Scopus subject areas

  • Periodontics

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