Effect of tetracycline HCl on periodontally-affected human root surfaces

L. Trombelli, A. Scabbia, F. Zangari, A. Griselli, U. M E Wikesjo, G. Calura

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Scanning electron microscopy was used to evaluate surface characteristics of periodontitis-exposed instrumented human cementum and dentin surfaces following topical application of tetracycline HCl (TTC). Specimens were randomly assigned to application of sterile saline for 1 minute (control); TTC (10 mg/ml) for 1 minute and 4 minutes, respectively; and TTC (100 mg/ml) for 1 minute and 4 minutes, respectively. Solutions were applied with a cotton pellet using a burnishing technique. Control specimens exhibited an amorphous irregular surface smear layer. TTC treatment of cementum for 1 minute resulted in a relatively debris-free, nonhomogeneous surface. The 4-minute application resulted in a surface exhibiting a densely fibrillar, mat-like texture. Dentin specimens conditioned for 1 minute showed a smooth surface with many tubule openings partially occluded by debris. The 4-minute treatment exposed a 3-dimensional network of intertubular and peritubular collagen fibrils. No consistent morphologic differences were observed between cementum or dentin specimens treated with TTC at concentrations of 10 and 100 mg/ml, respectively. The results suggest that topical application of TTC produces morphologic alterations of periodontitis-exposed cementum and dentin that appear related to application interval rather than concentration of the drug.

Original languageEnglish (US)
Pages (from-to)685-691
Number of pages7
JournalJournal of periodontology
Volume66
Issue number8
StatePublished - Jan 1 1995

Fingerprint

Tetracycline
Dental Cementum
Dentin
Periodontitis
Smear Layer
Electron Scanning Microscopy
Collagen
Pharmaceutical Preparations

Keywords

  • cementum/anatomy and histology
  • dentin/anatomy and histology
  • tetracycline/therapeutic use

ASJC Scopus subject areas

  • Periodontics

Cite this

Trombelli, L., Scabbia, A., Zangari, F., Griselli, A., Wikesjo, U. M. E., & Calura, G. (1995). Effect of tetracycline HCl on periodontally-affected human root surfaces. Journal of periodontology, 66(8), 685-691.

Effect of tetracycline HCl on periodontally-affected human root surfaces. / Trombelli, L.; Scabbia, A.; Zangari, F.; Griselli, A.; Wikesjo, U. M E; Calura, G.

In: Journal of periodontology, Vol. 66, No. 8, 01.01.1995, p. 685-691.

Research output: Contribution to journalArticle

Trombelli, L, Scabbia, A, Zangari, F, Griselli, A, Wikesjo, UME & Calura, G 1995, 'Effect of tetracycline HCl on periodontally-affected human root surfaces', Journal of periodontology, vol. 66, no. 8, pp. 685-691.
Trombelli L, Scabbia A, Zangari F, Griselli A, Wikesjo UME, Calura G. Effect of tetracycline HCl on periodontally-affected human root surfaces. Journal of periodontology. 1995 Jan 1;66(8):685-691.
Trombelli, L. ; Scabbia, A. ; Zangari, F. ; Griselli, A. ; Wikesjo, U. M E ; Calura, G. / Effect of tetracycline HCl on periodontally-affected human root surfaces. In: Journal of periodontology. 1995 ; Vol. 66, No. 8. pp. 685-691.
@article{b381b8baa5ec437ab0078fd1859866bc,
title = "Effect of tetracycline HCl on periodontally-affected human root surfaces",
abstract = "Scanning electron microscopy was used to evaluate surface characteristics of periodontitis-exposed instrumented human cementum and dentin surfaces following topical application of tetracycline HCl (TTC). Specimens were randomly assigned to application of sterile saline for 1 minute (control); TTC (10 mg/ml) for 1 minute and 4 minutes, respectively; and TTC (100 mg/ml) for 1 minute and 4 minutes, respectively. Solutions were applied with a cotton pellet using a burnishing technique. Control specimens exhibited an amorphous irregular surface smear layer. TTC treatment of cementum for 1 minute resulted in a relatively debris-free, nonhomogeneous surface. The 4-minute application resulted in a surface exhibiting a densely fibrillar, mat-like texture. Dentin specimens conditioned for 1 minute showed a smooth surface with many tubule openings partially occluded by debris. The 4-minute treatment exposed a 3-dimensional network of intertubular and peritubular collagen fibrils. No consistent morphologic differences were observed between cementum or dentin specimens treated with TTC at concentrations of 10 and 100 mg/ml, respectively. The results suggest that topical application of TTC produces morphologic alterations of periodontitis-exposed cementum and dentin that appear related to application interval rather than concentration of the drug.",
keywords = "cementum/anatomy and histology, dentin/anatomy and histology, tetracycline/therapeutic use",
author = "L. Trombelli and A. Scabbia and F. Zangari and A. Griselli and Wikesjo, {U. M E} and G. Calura",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "66",
pages = "685--691",
journal = "Journal of Periodontology",
issn = "0022-3492",
publisher = "American Academy of Periodontology",
number = "8",

}

TY - JOUR

T1 - Effect of tetracycline HCl on periodontally-affected human root surfaces

AU - Trombelli, L.

AU - Scabbia, A.

AU - Zangari, F.

AU - Griselli, A.

AU - Wikesjo, U. M E

AU - Calura, G.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Scanning electron microscopy was used to evaluate surface characteristics of periodontitis-exposed instrumented human cementum and dentin surfaces following topical application of tetracycline HCl (TTC). Specimens were randomly assigned to application of sterile saline for 1 minute (control); TTC (10 mg/ml) for 1 minute and 4 minutes, respectively; and TTC (100 mg/ml) for 1 minute and 4 minutes, respectively. Solutions were applied with a cotton pellet using a burnishing technique. Control specimens exhibited an amorphous irregular surface smear layer. TTC treatment of cementum for 1 minute resulted in a relatively debris-free, nonhomogeneous surface. The 4-minute application resulted in a surface exhibiting a densely fibrillar, mat-like texture. Dentin specimens conditioned for 1 minute showed a smooth surface with many tubule openings partially occluded by debris. The 4-minute treatment exposed a 3-dimensional network of intertubular and peritubular collagen fibrils. No consistent morphologic differences were observed between cementum or dentin specimens treated with TTC at concentrations of 10 and 100 mg/ml, respectively. The results suggest that topical application of TTC produces morphologic alterations of periodontitis-exposed cementum and dentin that appear related to application interval rather than concentration of the drug.

AB - Scanning electron microscopy was used to evaluate surface characteristics of periodontitis-exposed instrumented human cementum and dentin surfaces following topical application of tetracycline HCl (TTC). Specimens were randomly assigned to application of sterile saline for 1 minute (control); TTC (10 mg/ml) for 1 minute and 4 minutes, respectively; and TTC (100 mg/ml) for 1 minute and 4 minutes, respectively. Solutions were applied with a cotton pellet using a burnishing technique. Control specimens exhibited an amorphous irregular surface smear layer. TTC treatment of cementum for 1 minute resulted in a relatively debris-free, nonhomogeneous surface. The 4-minute application resulted in a surface exhibiting a densely fibrillar, mat-like texture. Dentin specimens conditioned for 1 minute showed a smooth surface with many tubule openings partially occluded by debris. The 4-minute treatment exposed a 3-dimensional network of intertubular and peritubular collagen fibrils. No consistent morphologic differences were observed between cementum or dentin specimens treated with TTC at concentrations of 10 and 100 mg/ml, respectively. The results suggest that topical application of TTC produces morphologic alterations of periodontitis-exposed cementum and dentin that appear related to application interval rather than concentration of the drug.

KW - cementum/anatomy and histology

KW - dentin/anatomy and histology

KW - tetracycline/therapeutic use

UR - http://www.scopus.com/inward/record.url?scp=0029090603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029090603&partnerID=8YFLogxK

M3 - Article

C2 - 7473011

AN - SCOPUS:0029090603

VL - 66

SP - 685

EP - 691

JO - Journal of Periodontology

JF - Journal of Periodontology

SN - 0022-3492

IS - 8

ER -