Effect of tobacco compounds on gene expression profiles in human epithelial cells

Sung Hwa Sohn, Jaebum Lee, Ki Nam Kim, In kyoung Kim, Meyoung Kon Kim

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

This study was carried out to investigate the effects of the tobacco compounds (TC), nicotine, B(a)P, and 2-naphthylamine, on gene expression profiles in a human epithelial cells (A549). We treated A549 with the TC and analyzed gene expression using microarray and real-time PCR (RTP). Gene expression varied according to the TC used. By microarray, we found that apoptosis-related genes such as apoptosis-associated tyrosine kinase, interleukin 10 receptor beta, caspase 1 and DNA fragmentation factor beta subunit (40 kDa) were down-regulated in TC-treated A549 cells. RTP showed significant increases in the expression of Ahr, Arnt, CYP1A1, and CYP1B1 in TC-treated A549 cells. From these results, we suggest that tobacco compounds can influence apoptosis, inflammation, immunity, and the cell cycle in A549 cells. Also, our study demonstrates that a microarray-based genomic survey is a suitable high-throughput approach for the evaluation of gene expression and for the characterization of TC-induced toxicity.

Original languageEnglish (US)
Pages (from-to)111-119
Number of pages9
JournalEnvironmental Toxicology and Pharmacology
Volume27
Issue number1
DOIs
Publication statusPublished - Jan 1 2009

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Keywords

  • 2-Naphthylamine
  • B(a)P
  • Gene expression
  • Microarray
  • Nicotine

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

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