Effect of ultraviolet A-induced crosslinking on dentin collagen matrix

Roda Seseogullari-Dirihan, Leo Tjäderhane, David Henry Pashley, Arzu Tezvergil-Mutluay

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives The aim of this study was to evaluate the effect of using UVA-induced crosslinking with or without riboflavin as photosensitizers on degradation of dentin matrix by dentin proteases. Methods Demineralized dentin specimens (0.4 × 3 × 6 mm3, n = 10/group) were subjected to: (RP1), 0.1% riboflavin-5 phosphate/UVA for 1 min; (RP5), 0.1% riboflavin-5 phosphate/UVA for 5 min; (R1), 0.1% riboflavin/UVA for 1 min; (R5), 0.1% riboflavin-UVA for 5 min; (UV1), UVA for 1 min; (UV5), UVA for 5 min. Specimens were incubated in 1 mL zinc and calcium containing media for 1 day and 1 week. An untreated group served as control (CM). After incubation, the loss of dry mass of samples was measured and aliquots of media were analyzed for the release of C-terminal fragment telopeptide (ICTP vs. CTX) of collagen to evaluate for cathepsin K (CA-K) and total matrix metalloproteinase (MMP)-mediated degradation. Data were analyzed using repeated measures ANOVA at α = 0.05. Results Although UVA radiation alone reduced dentin degradation, UVA-activated riboflavin or riboflavin-5 phosphate inhibited MMP and CA-K activities more than UVA alone. The effects of crosslinking were more pronounced in 7-day samples; only with CA-K were the effects of crosslinking with or without photosensitizer significantly different from controls in 1-day samples. Significance The use of bioactive forms (RP) or longer treatment time did not result with better effect. The use of UVA crosslinking reduces dentin matrix degradation, especially with photosensitizers.

Original languageEnglish (US)
Pages (from-to)1225-1231
Number of pages7
JournalDental Materials
Volume31
Issue number10
DOIs
StatePublished - Oct 1 2015

Fingerprint

Riboflavin
Cathepsin K
Dentin
Flavin Mononucleotide
Collagen
Crosslinking
Photosensitizing Agents
Photosensitizers
Degradation
Phosphates
Matrix Metalloproteinases
Analysis of variance (ANOVA)
Zinc
Calcium
Peptide Hydrolases
Analysis of Variance
Radiation
Cathepsins
Metalloproteases

Keywords

  • Collagen matrices
  • Crosslinking
  • Dentin
  • Riboflavin

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Seseogullari-Dirihan, R., Tjäderhane, L., Pashley, D. H., & Tezvergil-Mutluay, A. (2015). Effect of ultraviolet A-induced crosslinking on dentin collagen matrix. Dental Materials, 31(10), 1225-1231. https://doi.org/10.1016/j.dental.2015.08.145

Effect of ultraviolet A-induced crosslinking on dentin collagen matrix. / Seseogullari-Dirihan, Roda; Tjäderhane, Leo; Pashley, David Henry; Tezvergil-Mutluay, Arzu.

In: Dental Materials, Vol. 31, No. 10, 01.10.2015, p. 1225-1231.

Research output: Contribution to journalArticle

Seseogullari-Dirihan, R, Tjäderhane, L, Pashley, DH & Tezvergil-Mutluay, A 2015, 'Effect of ultraviolet A-induced crosslinking on dentin collagen matrix', Dental Materials, vol. 31, no. 10, pp. 1225-1231. https://doi.org/10.1016/j.dental.2015.08.145
Seseogullari-Dirihan R, Tjäderhane L, Pashley DH, Tezvergil-Mutluay A. Effect of ultraviolet A-induced crosslinking on dentin collagen matrix. Dental Materials. 2015 Oct 1;31(10):1225-1231. https://doi.org/10.1016/j.dental.2015.08.145
Seseogullari-Dirihan, Roda ; Tjäderhane, Leo ; Pashley, David Henry ; Tezvergil-Mutluay, Arzu. / Effect of ultraviolet A-induced crosslinking on dentin collagen matrix. In: Dental Materials. 2015 ; Vol. 31, No. 10. pp. 1225-1231.
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AB - Objectives The aim of this study was to evaluate the effect of using UVA-induced crosslinking with or without riboflavin as photosensitizers on degradation of dentin matrix by dentin proteases. Methods Demineralized dentin specimens (0.4 × 3 × 6 mm3, n = 10/group) were subjected to: (RP1), 0.1% riboflavin-5 phosphate/UVA for 1 min; (RP5), 0.1% riboflavin-5 phosphate/UVA for 5 min; (R1), 0.1% riboflavin/UVA for 1 min; (R5), 0.1% riboflavin-UVA for 5 min; (UV1), UVA for 1 min; (UV5), UVA for 5 min. Specimens were incubated in 1 mL zinc and calcium containing media for 1 day and 1 week. An untreated group served as control (CM). After incubation, the loss of dry mass of samples was measured and aliquots of media were analyzed for the release of C-terminal fragment telopeptide (ICTP vs. CTX) of collagen to evaluate for cathepsin K (CA-K) and total matrix metalloproteinase (MMP)-mediated degradation. Data were analyzed using repeated measures ANOVA at α = 0.05. Results Although UVA radiation alone reduced dentin degradation, UVA-activated riboflavin or riboflavin-5 phosphate inhibited MMP and CA-K activities more than UVA alone. The effects of crosslinking were more pronounced in 7-day samples; only with CA-K were the effects of crosslinking with or without photosensitizer significantly different from controls in 1-day samples. Significance The use of bioactive forms (RP) or longer treatment time did not result with better effect. The use of UVA crosslinking reduces dentin matrix degradation, especially with photosensitizers.

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