Effect of verapamil on cholesteatoma migration in vitro

Stilianos E Kountakis, C. Y.Joseph Chang, A. M. Minotti, Fernando R. Cabral

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

OBJECTIVES: It was previously shown that cholesteatoma migration in vitro is influenced by the calcium concentration of the culture medium. This study was designed to determine whether the calcium channel blocker verapamil affects cholesteatoma migration in vitro. METHODS: Cholesteatoma cells harvested from patients with chronic ear disease were grown in culture and were exposed to culture medium containing verapamil. The migration rate of the verapamil-exposed cells was compared with control rates. RESULTS: Verapamil at 300 μg/L caused marked reduction in the rate of migration compared with control values. The migration rate returned to normal within 48 hours after verapamil was removed from the culture medium. Higher verapamil concentrations (500 μg/L) caused complete detachment of the epithelial cells from the substrate within 24 hours. CONCLUSION: Our findings suggest that cholesteatoma migration in vitro is calcium channel dependent and can be reduced with calcium channel blockers such as verapamil.

Original languageEnglish (US)
Pages (from-to)91-95
Number of pages5
JournalOtolaryngology - Head and Neck Surgery
Volume122
Issue number1
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

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Cholesteatoma
Verapamil
Culture Media
Calcium Channel Blockers
Ear Diseases
Calcium Channels
In Vitro Techniques
Chronic Disease
Epithelial Cells
Calcium

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Effect of verapamil on cholesteatoma migration in vitro. / Kountakis, Stilianos E; Chang, C. Y.Joseph; Minotti, A. M.; Cabral, Fernando R.

In: Otolaryngology - Head and Neck Surgery, Vol. 122, No. 1, 01.01.2000, p. 91-95.

Research output: Contribution to journalArticle

Kountakis, Stilianos E ; Chang, C. Y.Joseph ; Minotti, A. M. ; Cabral, Fernando R. / Effect of verapamil on cholesteatoma migration in vitro. In: Otolaryngology - Head and Neck Surgery. 2000 ; Vol. 122, No. 1. pp. 91-95.
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