Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men

Jennifer M. Sacheck, Paul E. Milbury, Joseph Gerard Cannon, Ronenn Roubenoff, Jeffrey B. Blumberg

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 ± 3.3 years) and 16 older (71.1 ± 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75% VO2max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F-isoprostanes (iPF; p < .001) and malondialdehyde (MDA; p < .01), although iPF peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h (p < .01) and correlated with the rise in iPF, MDA, and CK in the young men (p < .05). Leukocyte 8-hydroxy-2′-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF and suppressed the 24 h postexercise increases in iPF (p < .05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.

Original languageEnglish (US)
Pages (from-to)1575-1588
Number of pages14
JournalFree Radical Biology and Medicine
Volume34
Issue number12
DOIs
StatePublished - Jun 15 2003

Fingerprint

Oxidative stress
Biomarkers
Creatine Kinase
Vitamin E
Oxidative Stress
Exercise
Muscle
F2-Isoprostanes
Malondialdehyde
Reactive Oxygen Species
Blood
Antioxidants
Muscles
Lipids
DNA
Lipid Peroxidation
DNA Damage
Leukocytes
Placebos
Wounds and Injuries

Keywords

  • Aging
  • Antioxidants
  • DNA damage
  • Exercise
  • Free radicals
  • Lipid peroxidation
  • Muscle damage
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men. / Sacheck, Jennifer M.; Milbury, Paul E.; Cannon, Joseph Gerard; Roubenoff, Ronenn; Blumberg, Jeffrey B.

In: Free Radical Biology and Medicine, Vol. 34, No. 12, 15.06.2003, p. 1575-1588.

Research output: Contribution to journalArticle

Sacheck, Jennifer M. ; Milbury, Paul E. ; Cannon, Joseph Gerard ; Roubenoff, Ronenn ; Blumberg, Jeffrey B. / Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men. In: Free Radical Biology and Medicine. 2003 ; Vol. 34, No. 12. pp. 1575-1588.
@article{c34f9699f1cb494a9f6c680df691e92a,
title = "Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men",
abstract = "Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 ± 3.3 years) and 16 older (71.1 ± 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75{\%} VO2max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F2α-isoprostanes (iPF2α; p < .001) and malondialdehyde (MDA; p < .01), although iPF2α peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h (p < .01) and correlated with the rise in iPF2α, MDA, and CK in the young men (p < .05). Leukocyte 8-hydroxy-2′-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF2α and suppressed the 24 h postexercise increases in iPF2α (p < .05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.",
keywords = "Aging, Antioxidants, DNA damage, Exercise, Free radicals, Lipid peroxidation, Muscle damage, Vitamin E",
author = "Sacheck, {Jennifer M.} and Milbury, {Paul E.} and Cannon, {Joseph Gerard} and Ronenn Roubenoff and Blumberg, {Jeffrey B.}",
year = "2003",
month = "6",
day = "15",
doi = "10.1016/S0891-5849(03)00187-4",
language = "English (US)",
volume = "34",
pages = "1575--1588",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "12",

}

TY - JOUR

T1 - Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men

AU - Sacheck, Jennifer M.

AU - Milbury, Paul E.

AU - Cannon, Joseph Gerard

AU - Roubenoff, Ronenn

AU - Blumberg, Jeffrey B.

PY - 2003/6/15

Y1 - 2003/6/15

N2 - Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 ± 3.3 years) and 16 older (71.1 ± 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75% VO2max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F2α-isoprostanes (iPF2α; p < .001) and malondialdehyde (MDA; p < .01), although iPF2α peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h (p < .01) and correlated with the rise in iPF2α, MDA, and CK in the young men (p < .05). Leukocyte 8-hydroxy-2′-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF2α and suppressed the 24 h postexercise increases in iPF2α (p < .05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.

AB - Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 ± 3.3 years) and 16 older (71.1 ± 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75% VO2max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F2α-isoprostanes (iPF2α; p < .001) and malondialdehyde (MDA; p < .01), although iPF2α peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h (p < .01) and correlated with the rise in iPF2α, MDA, and CK in the young men (p < .05). Leukocyte 8-hydroxy-2′-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF2α and suppressed the 24 h postexercise increases in iPF2α (p < .05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.

KW - Aging

KW - Antioxidants

KW - DNA damage

KW - Exercise

KW - Free radicals

KW - Lipid peroxidation

KW - Muscle damage

KW - Vitamin E

UR - http://www.scopus.com/inward/record.url?scp=0037683669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037683669&partnerID=8YFLogxK

U2 - 10.1016/S0891-5849(03)00187-4

DO - 10.1016/S0891-5849(03)00187-4

M3 - Article

VL - 34

SP - 1575

EP - 1588

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 12

ER -