Effect of whole-body vibration on bone properties in aging mice

Karl H. Wenger, James D. Freeman, Sadanand T Fulzele, David M. Immel, Brian D. Powell, Patrick Molitor, Yuh J. Chao, Hong Sheng Gao, Mohammed Elsayed Elsalanty, Mark W Hamrick, Carlos M Isales, Jack C Yu

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Recent studies suggest that whole-body vibration (WBV) can improve measures of bone health for certain clinical conditions and ages. In the elderly, there also is particular interest in assessing the ability of physical interventions such as WBV to improve coordination, strength, and movement speed, which help prevent falls and fractures and maintain ambulation for independent living. The current study evaluated the efficacy of WBV in an aging mouse model. Two levels of vibration - 0.5 and 1.5. g - were applied at 32. Hz to CB57BL/6 male mice (n=9 each) beginning at age 18. months and continuing for 12. weeks, 30. min/day, in a novel pivoting vibration device. Previous reports indicate that bone parameters in these mice begin to decrease substantially at 18. months, equivalent to mid-fifties for humans. Micro-computed tomography (micro-CT) and biomechanical assessments were made in the femur, radius, and lumbar vertebra to determine the effect of these WBV magnitudes and durations in the aging model. Sera also were collected for analysis of bone formation and breakdown markers. Mineralizing surface and cell counts were determined histologically.Bone volume in four regions of the femur did not change significantly, but there was a consistent shift toward higher mean density in the bone density spectrum (BDS), with the two vibration levels producing similar results. This new parameter represents an integral of the conventional density histogram. The amount of high density bone statistically improved in the head, neck, and diaphysis. Biomechanically, there was a trend toward greater stiffness in the 1.5. g group (p=0.139 vs. controls in the radius), and no change in strength. In the lumbar spine, no differences were seen due to vibration. Both vibration groups significantly reduced pyridinoline crosslinks, a collagen breakdown marker. They also significantly increased dynamic mineralization, MS/BS. Furthermore, osteoclasts were most numerous in the 1.5. g group (p≤0.05). These findings suggest that some benefits of WBV found in previous studies of young and mature rodent models may extend to an aging population. Density parameters indicated 0.5. g was more effective than 1.5. g. Serological markers, by contrast, favored 1.5. g, while biomechanically and histologically the results were mixed. Although the purported anabolic effect of WBV on bone homeostasis may depend on location and the parameter of interest, this emerging therapy at a minimum does not appear to compromise bone health by the measures studied here.

Original languageEnglish (US)
Pages (from-to)746-755
Number of pages10
JournalBone
Volume47
Issue number4
DOIs
StatePublished - Oct 1 2010

Fingerprint

Vibration
Bone and Bones
Bone Density
Femur
Independent Living
Anabolic Agents
Diaphyses
Lumbar Vertebrae
Health
Osteoclasts
Osteogenesis
Walking
Rodentia
Spine
Homeostasis
Neck
Collagen
Cell Count
Head
Tomography

Keywords

  • Aging
  • Bone biomechanics
  • Computed tomography
  • Femur
  • Whole-body vibration

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Wenger, K. H., Freeman, J. D., Fulzele, S. T., Immel, D. M., Powell, B. D., Molitor, P., ... Yu, J. C. (2010). Effect of whole-body vibration on bone properties in aging mice. Bone, 47(4), 746-755. https://doi.org/10.1016/j.bone.2010.07.014

Effect of whole-body vibration on bone properties in aging mice. / Wenger, Karl H.; Freeman, James D.; Fulzele, Sadanand T; Immel, David M.; Powell, Brian D.; Molitor, Patrick; Chao, Yuh J.; Gao, Hong Sheng; Elsalanty, Mohammed Elsayed; Hamrick, Mark W; Isales, Carlos M; Yu, Jack C.

In: Bone, Vol. 47, No. 4, 01.10.2010, p. 746-755.

Research output: Contribution to journalArticle

Wenger, KH, Freeman, JD, Fulzele, ST, Immel, DM, Powell, BD, Molitor, P, Chao, YJ, Gao, HS, Elsalanty, ME, Hamrick, MW, Isales, CM & Yu, JC 2010, 'Effect of whole-body vibration on bone properties in aging mice', Bone, vol. 47, no. 4, pp. 746-755. https://doi.org/10.1016/j.bone.2010.07.014
Wenger KH, Freeman JD, Fulzele ST, Immel DM, Powell BD, Molitor P et al. Effect of whole-body vibration on bone properties in aging mice. Bone. 2010 Oct 1;47(4):746-755. https://doi.org/10.1016/j.bone.2010.07.014
Wenger, Karl H. ; Freeman, James D. ; Fulzele, Sadanand T ; Immel, David M. ; Powell, Brian D. ; Molitor, Patrick ; Chao, Yuh J. ; Gao, Hong Sheng ; Elsalanty, Mohammed Elsayed ; Hamrick, Mark W ; Isales, Carlos M ; Yu, Jack C. / Effect of whole-body vibration on bone properties in aging mice. In: Bone. 2010 ; Vol. 47, No. 4. pp. 746-755.
@article{5163593136c340d9b0dd07ab2022b4d3,
title = "Effect of whole-body vibration on bone properties in aging mice",
abstract = "Recent studies suggest that whole-body vibration (WBV) can improve measures of bone health for certain clinical conditions and ages. In the elderly, there also is particular interest in assessing the ability of physical interventions such as WBV to improve coordination, strength, and movement speed, which help prevent falls and fractures and maintain ambulation for independent living. The current study evaluated the efficacy of WBV in an aging mouse model. Two levels of vibration - 0.5 and 1.5. g - were applied at 32. Hz to CB57BL/6 male mice (n=9 each) beginning at age 18. months and continuing for 12. weeks, 30. min/day, in a novel pivoting vibration device. Previous reports indicate that bone parameters in these mice begin to decrease substantially at 18. months, equivalent to mid-fifties for humans. Micro-computed tomography (micro-CT) and biomechanical assessments were made in the femur, radius, and lumbar vertebra to determine the effect of these WBV magnitudes and durations in the aging model. Sera also were collected for analysis of bone formation and breakdown markers. Mineralizing surface and cell counts were determined histologically.Bone volume in four regions of the femur did not change significantly, but there was a consistent shift toward higher mean density in the bone density spectrum (BDS), with the two vibration levels producing similar results. This new parameter represents an integral of the conventional density histogram. The amount of high density bone statistically improved in the head, neck, and diaphysis. Biomechanically, there was a trend toward greater stiffness in the 1.5. g group (p=0.139 vs. controls in the radius), and no change in strength. In the lumbar spine, no differences were seen due to vibration. Both vibration groups significantly reduced pyridinoline crosslinks, a collagen breakdown marker. They also significantly increased dynamic mineralization, MS/BS. Furthermore, osteoclasts were most numerous in the 1.5. g group (p≤0.05). These findings suggest that some benefits of WBV found in previous studies of young and mature rodent models may extend to an aging population. Density parameters indicated 0.5. g was more effective than 1.5. g. Serological markers, by contrast, favored 1.5. g, while biomechanically and histologically the results were mixed. Although the purported anabolic effect of WBV on bone homeostasis may depend on location and the parameter of interest, this emerging therapy at a minimum does not appear to compromise bone health by the measures studied here.",
keywords = "Aging, Bone biomechanics, Computed tomography, Femur, Whole-body vibration",
author = "Wenger, {Karl H.} and Freeman, {James D.} and Fulzele, {Sadanand T} and Immel, {David M.} and Powell, {Brian D.} and Patrick Molitor and Chao, {Yuh J.} and Gao, {Hong Sheng} and Elsalanty, {Mohammed Elsayed} and Hamrick, {Mark W} and Isales, {Carlos M} and Yu, {Jack C}",
year = "2010",
month = "10",
day = "1",
doi = "10.1016/j.bone.2010.07.014",
language = "English (US)",
volume = "47",
pages = "746--755",
journal = "Bone",
issn = "8756-3282",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Effect of whole-body vibration on bone properties in aging mice

AU - Wenger, Karl H.

AU - Freeman, James D.

AU - Fulzele, Sadanand T

AU - Immel, David M.

AU - Powell, Brian D.

AU - Molitor, Patrick

AU - Chao, Yuh J.

AU - Gao, Hong Sheng

AU - Elsalanty, Mohammed Elsayed

AU - Hamrick, Mark W

AU - Isales, Carlos M

AU - Yu, Jack C

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Recent studies suggest that whole-body vibration (WBV) can improve measures of bone health for certain clinical conditions and ages. In the elderly, there also is particular interest in assessing the ability of physical interventions such as WBV to improve coordination, strength, and movement speed, which help prevent falls and fractures and maintain ambulation for independent living. The current study evaluated the efficacy of WBV in an aging mouse model. Two levels of vibration - 0.5 and 1.5. g - were applied at 32. Hz to CB57BL/6 male mice (n=9 each) beginning at age 18. months and continuing for 12. weeks, 30. min/day, in a novel pivoting vibration device. Previous reports indicate that bone parameters in these mice begin to decrease substantially at 18. months, equivalent to mid-fifties for humans. Micro-computed tomography (micro-CT) and biomechanical assessments were made in the femur, radius, and lumbar vertebra to determine the effect of these WBV magnitudes and durations in the aging model. Sera also were collected for analysis of bone formation and breakdown markers. Mineralizing surface and cell counts were determined histologically.Bone volume in four regions of the femur did not change significantly, but there was a consistent shift toward higher mean density in the bone density spectrum (BDS), with the two vibration levels producing similar results. This new parameter represents an integral of the conventional density histogram. The amount of high density bone statistically improved in the head, neck, and diaphysis. Biomechanically, there was a trend toward greater stiffness in the 1.5. g group (p=0.139 vs. controls in the radius), and no change in strength. In the lumbar spine, no differences were seen due to vibration. Both vibration groups significantly reduced pyridinoline crosslinks, a collagen breakdown marker. They also significantly increased dynamic mineralization, MS/BS. Furthermore, osteoclasts were most numerous in the 1.5. g group (p≤0.05). These findings suggest that some benefits of WBV found in previous studies of young and mature rodent models may extend to an aging population. Density parameters indicated 0.5. g was more effective than 1.5. g. Serological markers, by contrast, favored 1.5. g, while biomechanically and histologically the results were mixed. Although the purported anabolic effect of WBV on bone homeostasis may depend on location and the parameter of interest, this emerging therapy at a minimum does not appear to compromise bone health by the measures studied here.

AB - Recent studies suggest that whole-body vibration (WBV) can improve measures of bone health for certain clinical conditions and ages. In the elderly, there also is particular interest in assessing the ability of physical interventions such as WBV to improve coordination, strength, and movement speed, which help prevent falls and fractures and maintain ambulation for independent living. The current study evaluated the efficacy of WBV in an aging mouse model. Two levels of vibration - 0.5 and 1.5. g - were applied at 32. Hz to CB57BL/6 male mice (n=9 each) beginning at age 18. months and continuing for 12. weeks, 30. min/day, in a novel pivoting vibration device. Previous reports indicate that bone parameters in these mice begin to decrease substantially at 18. months, equivalent to mid-fifties for humans. Micro-computed tomography (micro-CT) and biomechanical assessments were made in the femur, radius, and lumbar vertebra to determine the effect of these WBV magnitudes and durations in the aging model. Sera also were collected for analysis of bone formation and breakdown markers. Mineralizing surface and cell counts were determined histologically.Bone volume in four regions of the femur did not change significantly, but there was a consistent shift toward higher mean density in the bone density spectrum (BDS), with the two vibration levels producing similar results. This new parameter represents an integral of the conventional density histogram. The amount of high density bone statistically improved in the head, neck, and diaphysis. Biomechanically, there was a trend toward greater stiffness in the 1.5. g group (p=0.139 vs. controls in the radius), and no change in strength. In the lumbar spine, no differences were seen due to vibration. Both vibration groups significantly reduced pyridinoline crosslinks, a collagen breakdown marker. They also significantly increased dynamic mineralization, MS/BS. Furthermore, osteoclasts were most numerous in the 1.5. g group (p≤0.05). These findings suggest that some benefits of WBV found in previous studies of young and mature rodent models may extend to an aging population. Density parameters indicated 0.5. g was more effective than 1.5. g. Serological markers, by contrast, favored 1.5. g, while biomechanically and histologically the results were mixed. Although the purported anabolic effect of WBV on bone homeostasis may depend on location and the parameter of interest, this emerging therapy at a minimum does not appear to compromise bone health by the measures studied here.

KW - Aging

KW - Bone biomechanics

KW - Computed tomography

KW - Femur

KW - Whole-body vibration

UR - http://www.scopus.com/inward/record.url?scp=77956414227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956414227&partnerID=8YFLogxK

U2 - 10.1016/j.bone.2010.07.014

DO - 10.1016/j.bone.2010.07.014

M3 - Article

C2 - 20638490

AN - SCOPUS:77956414227

VL - 47

SP - 746

EP - 755

JO - Bone

JF - Bone

SN - 8756-3282

IS - 4

ER -