Effective optimization of SARS-CoV-2 laboratory testing variables in an era of supply chain constraints

Nikhil S Sahajpal, Ashis K. Mondal, Allan Njau, Sudha Ananth, Kimya Jones, Pankaj K. Ahluwalia, Meenakshi Ahluwalia, Yasmeen Jilani, Alka Chaubey, Madhuri Hegde, Vamsi Kota, Amyn Rojiani, Ravindra Kolhe

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations

Abstract

RT-PCR-based assays for the detection of SARS-CoV-2 have played an essential role in the current COVID-19 pandemic. However, the sample collection and test reagents are in short supply, primarily due to supply chain issues. Thus, to eliminate testing constraints, we have optimized three key process variables: RNA extraction and RT-PCR reactions, different sample types and media to facilitate SARS-CoV-2 testing. By performing various validation and bridging studies, we have shown that various sample types such as nasopharyngeal swab, bronchioalveolar lavage and saliva, collected using conventional nasopharyngeal swabs, ESwab or 3D-printed swabs and, preserved in viral transport media, universal transport media, 0.9% sodium chloride or Amies media are compatible with RT-PCR assay for COVID-19. Besides, the reduction of PCR reagents by up to fourfold also produces reliable results.

Original languageEnglish (US)
Pages (from-to)1483-1487
Number of pages5
JournalFuture Microbiology
Volume15
Issue number15
DOIs
StatePublished - Oct 2020

Keywords

  • 3D swab
  • COVID-19
  • assay modifications
  • assay optimization
  • bridging studies

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Effective optimization of SARS-CoV-2 laboratory testing variables in an era of supply chain constraints'. Together they form a unique fingerprint.

Cite this