Effects of a discoloration-resistant calcium aluminosilicate cement on the viability and proliferation of undifferentiated human dental pulp stem cells

Li Na Niu, Devon Watson, Kyle Thames, Carolyn M. Primus, Brian Edward Bergeron, Kai Jiao, Eduardo A. Bortoluzzi, Christopher W Cutler, Ji Hua Chen, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

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Abstract

Discoloration-resistant calcium aluminosilicate cement has been formulated to overcome the timely problem of tooth discoloration reported in the clinical application of bismuth oxide-containing hydraulic cements. The present study examined the effects of this experimental cement (Quick-Set2) on the viability and proliferation of human dental pulp stem cells (hDPSCs) by comparing the cellular responses with commercially available calcium silicate cement (white mineral trioxide aggregate; WMTA) after different aging periods. Cell viability and proliferation were examined using assays that examined plasma membrane integrity, leakage of cytosolic enzyme, caspase-3 activity for early apoptosis, oxidative stress, mitochondrial metabolic activity and intracellular DNA content. Results of the six assays indicated that both Quick-Set2 and WMTA were initially cytotoxic to hDPSCs after setting for 24 h, with Quick-Set2 being comparatively less cytotoxic than WMTA at this stage. After two aging cycles, the cytotoxicity profiles of the two hydraulic cements were not significantly different and were much less cytotoxic than the positive control (zinc oxide-eugenol cement). Based on these results, it is envisaged that any potential beneficial effect of the discoloration-resistant calcium aluminosilicate cement on osteogenesis by differentiated hDPSCs is more likely to be revealed after outward diffusion and removal of its cytotoxic components.

Original languageEnglish (US)
Article number17177
JournalScientific Reports
Volume5
DOIs
StatePublished - Nov 30 2015

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Dental Pulp
Stem Cells
Zinc Oxide-Eugenol Cement
Tooth Discoloration
Silicate Cement
Osteogenesis
Caspase 3
Cell Survival
Oxidative Stress
Cell Proliferation
Cell Membrane
Apoptosis
DNA
Enzymes
calcium aluminosilicate

ASJC Scopus subject areas

  • General

Cite this

Effects of a discoloration-resistant calcium aluminosilicate cement on the viability and proliferation of undifferentiated human dental pulp stem cells. / Niu, Li Na; Watson, Devon; Thames, Kyle; Primus, Carolyn M.; Bergeron, Brian Edward; Jiao, Kai; Bortoluzzi, Eduardo A.; Cutler, Christopher W; Chen, Ji Hua; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Scientific Reports, Vol. 5, 17177, 30.11.2015.

Research output: Contribution to journalArticle

Niu, Li Na ; Watson, Devon ; Thames, Kyle ; Primus, Carolyn M. ; Bergeron, Brian Edward ; Jiao, Kai ; Bortoluzzi, Eduardo A. ; Cutler, Christopher W ; Chen, Ji Hua ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Effects of a discoloration-resistant calcium aluminosilicate cement on the viability and proliferation of undifferentiated human dental pulp stem cells. In: Scientific Reports. 2015 ; Vol. 5.
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