Effects of acrylic resin monomers on porcine coronary artery reactivity

Worku Abebe, Daniel West, Frederick Rueggeberg, David Henry Pashley, Mahmood S Mozaffari

Research output: Contribution to journalArticle

Abstract

Abstract: The purpose of the present investigation was to assess the reactivity of porcine coronary arteries under in vitro conditions following their exposure to methyl methacrylate (MMA) and hydroxyethyl methacrylate (HEMA) monomers. Confirming previous studies using rat aortas, both MMA and HEMA induced acute/direct relaxation of coronary ring preparations, which was partly dependent on the endothelium. With prolonged tissue exposure, both monomers caused time- and concentration-dependent inhibition of receptor-mediated contraction of the vascular smooth muscle caused by prostaglandin F2∝ (PGF2∝), with HEMA causing more inhibition than MMA. Hydroxyethyl methacrylate, but not MMA, also produced impairment of non-receptor-mediated contraction of the coronary smooth muscle induced by KCl. On the other hand, neither HEMA nor MMA altered relaxation of the smooth muscle produced by the direct-acting pharmacological agent, sodium nitroprusside (SNP). While exposure to HEMA impaired endothelium-dependent vasorelaxation caused by bradykinin (BK), MMA markedly enhanced this endothelial-mediated response of the arteries. The enhanced endothelial response produced by MMA was linked to nitric oxide (NO) release. In conclusion, with prolonged tissue exposure, MMA causes less pronounced effects/adverse consequences on coronary smooth muscle function relative to the effect of HEMA, while enhancing vasorelaxation associated with release of NO from the endothelium. Accordingly, MMA-containing resin materials appear to be safer for human applications than materials containing HEMA.

Original languageEnglish (US)
Pages (from-to)986-1002
Number of pages17
JournalJournal of Biomaterials Science, Polymer Edition
Volume27
Issue number10
DOIs
StatePublished - Jul 2 2016

Fingerprint

Acrylic Resins
Methacrylates
Acrylics
Muscle
Coronary Vessels
Swine
Resins
Monomers
Nitric oxide
Tissue
Endothelium
Smooth Muscle
Rats
Vasodilation
Nitric Oxide
Sodium
Dinoprost
hydroxyethyl methacrylate
Nitroprusside
Bradykinin

Keywords

  • HEMA
  • MMA
  • Resin monomers
  • methacrylate biocompatibility
  • vascular reactivity

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Effects of acrylic resin monomers on porcine coronary artery reactivity. / Abebe, Worku; West, Daniel; Rueggeberg, Frederick; Pashley, David Henry; Mozaffari, Mahmood S.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 27, No. 10, 02.07.2016, p. 986-1002.

Research output: Contribution to journalArticle

@article{79c92cd01aec4e66b0ae87ee46d2f6b8,
title = "Effects of acrylic resin monomers on porcine coronary artery reactivity",
abstract = "Abstract: The purpose of the present investigation was to assess the reactivity of porcine coronary arteries under in vitro conditions following their exposure to methyl methacrylate (MMA) and hydroxyethyl methacrylate (HEMA) monomers. Confirming previous studies using rat aortas, both MMA and HEMA induced acute/direct relaxation of coronary ring preparations, which was partly dependent on the endothelium. With prolonged tissue exposure, both monomers caused time- and concentration-dependent inhibition of receptor-mediated contraction of the vascular smooth muscle caused by prostaglandin F2∝ (PGF2∝), with HEMA causing more inhibition than MMA. Hydroxyethyl methacrylate, but not MMA, also produced impairment of non-receptor-mediated contraction of the coronary smooth muscle induced by KCl. On the other hand, neither HEMA nor MMA altered relaxation of the smooth muscle produced by the direct-acting pharmacological agent, sodium nitroprusside (SNP). While exposure to HEMA impaired endothelium-dependent vasorelaxation caused by bradykinin (BK), MMA markedly enhanced this endothelial-mediated response of the arteries. The enhanced endothelial response produced by MMA was linked to nitric oxide (NO) release. In conclusion, with prolonged tissue exposure, MMA causes less pronounced effects/adverse consequences on coronary smooth muscle function relative to the effect of HEMA, while enhancing vasorelaxation associated with release of NO from the endothelium. Accordingly, MMA-containing resin materials appear to be safer for human applications than materials containing HEMA.",
keywords = "HEMA, MMA, Resin monomers, methacrylate biocompatibility, vascular reactivity",
author = "Worku Abebe and Daniel West and Frederick Rueggeberg and Pashley, {David Henry} and Mozaffari, {Mahmood S}",
year = "2016",
month = "7",
day = "2",
doi = "10.1080/09205063.2016.1175775",
language = "English (US)",
volume = "27",
pages = "986--1002",
journal = "Journal of Biomaterials Science, Polymer Edition",
issn = "0920-5063",
publisher = "Taylor and Francis Ltd.",
number = "10",

}

TY - JOUR

T1 - Effects of acrylic resin monomers on porcine coronary artery reactivity

AU - Abebe, Worku

AU - West, Daniel

AU - Rueggeberg, Frederick

AU - Pashley, David Henry

AU - Mozaffari, Mahmood S

PY - 2016/7/2

Y1 - 2016/7/2

N2 - Abstract: The purpose of the present investigation was to assess the reactivity of porcine coronary arteries under in vitro conditions following their exposure to methyl methacrylate (MMA) and hydroxyethyl methacrylate (HEMA) monomers. Confirming previous studies using rat aortas, both MMA and HEMA induced acute/direct relaxation of coronary ring preparations, which was partly dependent on the endothelium. With prolonged tissue exposure, both monomers caused time- and concentration-dependent inhibition of receptor-mediated contraction of the vascular smooth muscle caused by prostaglandin F2∝ (PGF2∝), with HEMA causing more inhibition than MMA. Hydroxyethyl methacrylate, but not MMA, also produced impairment of non-receptor-mediated contraction of the coronary smooth muscle induced by KCl. On the other hand, neither HEMA nor MMA altered relaxation of the smooth muscle produced by the direct-acting pharmacological agent, sodium nitroprusside (SNP). While exposure to HEMA impaired endothelium-dependent vasorelaxation caused by bradykinin (BK), MMA markedly enhanced this endothelial-mediated response of the arteries. The enhanced endothelial response produced by MMA was linked to nitric oxide (NO) release. In conclusion, with prolonged tissue exposure, MMA causes less pronounced effects/adverse consequences on coronary smooth muscle function relative to the effect of HEMA, while enhancing vasorelaxation associated with release of NO from the endothelium. Accordingly, MMA-containing resin materials appear to be safer for human applications than materials containing HEMA.

AB - Abstract: The purpose of the present investigation was to assess the reactivity of porcine coronary arteries under in vitro conditions following their exposure to methyl methacrylate (MMA) and hydroxyethyl methacrylate (HEMA) monomers. Confirming previous studies using rat aortas, both MMA and HEMA induced acute/direct relaxation of coronary ring preparations, which was partly dependent on the endothelium. With prolonged tissue exposure, both monomers caused time- and concentration-dependent inhibition of receptor-mediated contraction of the vascular smooth muscle caused by prostaglandin F2∝ (PGF2∝), with HEMA causing more inhibition than MMA. Hydroxyethyl methacrylate, but not MMA, also produced impairment of non-receptor-mediated contraction of the coronary smooth muscle induced by KCl. On the other hand, neither HEMA nor MMA altered relaxation of the smooth muscle produced by the direct-acting pharmacological agent, sodium nitroprusside (SNP). While exposure to HEMA impaired endothelium-dependent vasorelaxation caused by bradykinin (BK), MMA markedly enhanced this endothelial-mediated response of the arteries. The enhanced endothelial response produced by MMA was linked to nitric oxide (NO) release. In conclusion, with prolonged tissue exposure, MMA causes less pronounced effects/adverse consequences on coronary smooth muscle function relative to the effect of HEMA, while enhancing vasorelaxation associated with release of NO from the endothelium. Accordingly, MMA-containing resin materials appear to be safer for human applications than materials containing HEMA.

KW - HEMA

KW - MMA

KW - Resin monomers

KW - methacrylate biocompatibility

KW - vascular reactivity

UR - http://www.scopus.com/inward/record.url?scp=84965000279&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84965000279&partnerID=8YFLogxK

U2 - 10.1080/09205063.2016.1175775

DO - 10.1080/09205063.2016.1175775

M3 - Article

C2 - 27132475

AN - SCOPUS:84965000279

VL - 27

SP - 986

EP - 1002

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

SN - 0920-5063

IS - 10

ER -