Effects of ammonium hexafluorosilicate application on demineralized enamel and dentin of primary teeth.

Yumiko Hosoya, Eiko Watanabe, Katsumi Tadokoro, Takashi Inoue, Masashi Miyazaki, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Silver diamine fluoride (Ag(NH(3))(2)F) arrests caries but stains teeth black. To overcome this drawback, we applied ammonium hexafluorosilicate (AHF; (NH(4))(2)SiF(6)) and observed changes in the color and structure of demineralized enamel and dentin of extracted primary teeth. Enamel and dentin were demineralized in 10% EDTA solution for 90 s followed by 35% phosphoric acid gel for 60 s, then soaked in AHF solution for 60 s. Before analysis by scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDS), enamel and dentin were demineralized in 10% EDTA for 90 s. Teeth were divided into 4 groups according to AHF application and artificial saliva immersion status and then examined. The data were statistically analyzed using one-way ANOVA and Fisher's PLSD test at a significance level of P < 0.05. AHF treatment did not cause visible discoloration. Enamel prisms and dental tubules appeared by demineralization were covered with precipitates by AHF application. A sphere-filled membranous structure was observed in the saliva immersion groups. EDS analysis showed that AHF application had no effect on enamel; however, F% and Ca/P ratio were significantly higher on dentin surfaces after AHF application without artificial saliva immersion. Further study on arresting caries treatment is required.

Original languageEnglish (US)
Pages (from-to)267-272
Number of pages6
JournalJournal of oral science
Volume54
Issue number3
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Fingerprint

Deciduous Tooth
Dentin
Dental Enamel
Immersion
Artificial Saliva
Edetic Acid
Tooth
X-Ray Emission Spectrometry
Saliva
Electron Scanning Microscopy
Analysis of Variance
Coloring Agents
Color
Gels
hexafluorosilicate

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effects of ammonium hexafluorosilicate application on demineralized enamel and dentin of primary teeth. / Hosoya, Yumiko; Watanabe, Eiko; Tadokoro, Katsumi; Inoue, Takashi; Miyazaki, Masashi; Tay, Franklin Chi Meng.

In: Journal of oral science, Vol. 54, No. 3, 01.01.2012, p. 267-272.

Research output: Contribution to journalArticle

Hosoya, Yumiko ; Watanabe, Eiko ; Tadokoro, Katsumi ; Inoue, Takashi ; Miyazaki, Masashi ; Tay, Franklin Chi Meng. / Effects of ammonium hexafluorosilicate application on demineralized enamel and dentin of primary teeth. In: Journal of oral science. 2012 ; Vol. 54, No. 3. pp. 267-272.
@article{7c2d346e039d4ccbb5aee1e2776227ba,
title = "Effects of ammonium hexafluorosilicate application on demineralized enamel and dentin of primary teeth.",
abstract = "Silver diamine fluoride (Ag(NH(3))(2)F) arrests caries but stains teeth black. To overcome this drawback, we applied ammonium hexafluorosilicate (AHF; (NH(4))(2)SiF(6)) and observed changes in the color and structure of demineralized enamel and dentin of extracted primary teeth. Enamel and dentin were demineralized in 10{\%} EDTA solution for 90 s followed by 35{\%} phosphoric acid gel for 60 s, then soaked in AHF solution for 60 s. Before analysis by scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDS), enamel and dentin were demineralized in 10{\%} EDTA for 90 s. Teeth were divided into 4 groups according to AHF application and artificial saliva immersion status and then examined. The data were statistically analyzed using one-way ANOVA and Fisher's PLSD test at a significance level of P < 0.05. AHF treatment did not cause visible discoloration. Enamel prisms and dental tubules appeared by demineralization were covered with precipitates by AHF application. A sphere-filled membranous structure was observed in the saliva immersion groups. EDS analysis showed that AHF application had no effect on enamel; however, F{\%} and Ca/P ratio were significantly higher on dentin surfaces after AHF application without artificial saliva immersion. Further study on arresting caries treatment is required.",
author = "Yumiko Hosoya and Eiko Watanabe and Katsumi Tadokoro and Takashi Inoue and Masashi Miyazaki and Tay, {Franklin Chi Meng}",
year = "2012",
month = "1",
day = "1",
doi = "10.2334/josnusd.54.267",
language = "English (US)",
volume = "54",
pages = "267--272",
journal = "Journal of oral science",
issn = "1343-4934",
publisher = "Nihon University",
number = "3",

}

TY - JOUR

T1 - Effects of ammonium hexafluorosilicate application on demineralized enamel and dentin of primary teeth.

AU - Hosoya, Yumiko

AU - Watanabe, Eiko

AU - Tadokoro, Katsumi

AU - Inoue, Takashi

AU - Miyazaki, Masashi

AU - Tay, Franklin Chi Meng

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Silver diamine fluoride (Ag(NH(3))(2)F) arrests caries but stains teeth black. To overcome this drawback, we applied ammonium hexafluorosilicate (AHF; (NH(4))(2)SiF(6)) and observed changes in the color and structure of demineralized enamel and dentin of extracted primary teeth. Enamel and dentin were demineralized in 10% EDTA solution for 90 s followed by 35% phosphoric acid gel for 60 s, then soaked in AHF solution for 60 s. Before analysis by scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDS), enamel and dentin were demineralized in 10% EDTA for 90 s. Teeth were divided into 4 groups according to AHF application and artificial saliva immersion status and then examined. The data were statistically analyzed using one-way ANOVA and Fisher's PLSD test at a significance level of P < 0.05. AHF treatment did not cause visible discoloration. Enamel prisms and dental tubules appeared by demineralization were covered with precipitates by AHF application. A sphere-filled membranous structure was observed in the saliva immersion groups. EDS analysis showed that AHF application had no effect on enamel; however, F% and Ca/P ratio were significantly higher on dentin surfaces after AHF application without artificial saliva immersion. Further study on arresting caries treatment is required.

AB - Silver diamine fluoride (Ag(NH(3))(2)F) arrests caries but stains teeth black. To overcome this drawback, we applied ammonium hexafluorosilicate (AHF; (NH(4))(2)SiF(6)) and observed changes in the color and structure of demineralized enamel and dentin of extracted primary teeth. Enamel and dentin were demineralized in 10% EDTA solution for 90 s followed by 35% phosphoric acid gel for 60 s, then soaked in AHF solution for 60 s. Before analysis by scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDS), enamel and dentin were demineralized in 10% EDTA for 90 s. Teeth were divided into 4 groups according to AHF application and artificial saliva immersion status and then examined. The data were statistically analyzed using one-way ANOVA and Fisher's PLSD test at a significance level of P < 0.05. AHF treatment did not cause visible discoloration. Enamel prisms and dental tubules appeared by demineralization were covered with precipitates by AHF application. A sphere-filled membranous structure was observed in the saliva immersion groups. EDS analysis showed that AHF application had no effect on enamel; however, F% and Ca/P ratio were significantly higher on dentin surfaces after AHF application without artificial saliva immersion. Further study on arresting caries treatment is required.

UR - http://www.scopus.com/inward/record.url?scp=84883293955&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883293955&partnerID=8YFLogxK

U2 - 10.2334/josnusd.54.267

DO - 10.2334/josnusd.54.267

M3 - Article

VL - 54

SP - 267

EP - 272

JO - Journal of oral science

JF - Journal of oral science

SN - 1343-4934

IS - 3

ER -