Effects of amniotic fluid on proteases: A possible role of amniotic fluid in fetal wound healing

X. Gao, Lawrence D Devoe, K. S. Given, T. A. Mustoe

Research output: Contribution to journalArticle

17 Scopus citations


Fetuses are surrounded by amniotic fluid rich in nutrients and other factors essential to fetal development and possibly to its scarless wound healing. The purpose of this study was to determine whether amniotic fluid contains factors that regulate activities of major proteases involved in the process of wound healing (e.g., collagenase, hyaluronidase, elastase, and cathepsin B). Human amniotic fluid was assayed in vitro for inhibition or stimulation of the activity of these enzymes. Our results showed that amniotic fluid enhanced collagenase activity at a concentration of 66 μg protein (p < 0.01), but inhibited activities of hyaluronidase (132 μg protein; p < 0.05), elastase (170 μg protein; p < 0.05), and cathepsin B (19 μg protein; p < 0.01). This finding suggests that amniotic fluid could have an important role in flawless fetal wound healing by regulating these matrix- degrading enzymes.

Original languageEnglish (US)
Pages (from-to)128-135
Number of pages8
JournalAnnals of Plastic Surgery
Issue number2
StatePublished - Jan 1 1994


ASJC Scopus subject areas

  • Surgery

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