Effects of an experimental calcium aluminosilicate cement on the viability of murine odontoblast-like cells

Wei Wei, Yi Pin Qi, Sergey Y. Nikonov, Li Na Niu, Regina L W Messer, Jing Mao, Carolyn M. Primus, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Introduction: Quick-setting calcium aluminosilicate cement with improved washout resistance is a potential substitute for calcium silicate cements in endodontics. This study examined the effect of an experimental calcium aluminosilicate cement (Quick-Set; Primus Consulting, Bradenton, FL) on the viability of odontoblast-like cells. Methods: The biocompatibility of Quick-Set and white ProRoot MTA (WMTA; Dentsply Tulsa Dental Specialties, Tulsa, OK) cements and their eluents was evaluated using a murine dental papilla-derived odontoblast-like cell line (MDPC-23); 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was used to examine the effects of the 2 hydraulic cements on mitochondrial metabolic activity. Flow cytometry and confocal laser scanning microscopy were used to identify the effects of the 2 cements on cell death-induced plasma membrane permeability to fluorescent dyes and DNA stains. Results: After the first week of immersion in culture medium, Quick-Set and WMTA were more cytotoxic than the Teflon-negative control (P <.05), and the cells exhibited more apoptosis/necrosis than Teflon (P <.05). After the second week of immersion, the 2 cements were as biocompatible as Teflon (P >.05), with cells exhibiting minimal apoptosis/necrosis. Eluents from the set cements at 1:1 dilution were significantly more cytotoxic that eluents at 1:10 or 1:100 dilution (P <.05). Conclusions: Quick-Set and WMTA exhibited similar cytotoxicity profiles. They possess negligible in vitro toxicologic risks after time-dependent elution of toxic components.

Original languageEnglish (US)
Pages (from-to)936-942
Number of pages7
JournalJournal of Endodontics
Volume38
Issue number7
DOIs
StatePublished - Jul 1 2012

Fingerprint

Odontoblasts
Pemetrexed
Dental Specialties
Silicate Cement
Dental Papilla
Poisons
Endodontics
Polytetrafluoroethylene
Immersion
Fluorescent Dyes
Confocal Microscopy
Culture Media
Permeability
Flow Cytometry
Cell Death
Necrosis
Coloring Agents
Cell Membrane
Apoptosis
Cell Line

Keywords

  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
  • MTT assay
  • biocompatibility
  • calcium aluminate
  • flow cytometry
  • hydraulic cement
  • vital cell staining

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effects of an experimental calcium aluminosilicate cement on the viability of murine odontoblast-like cells. / Wei, Wei; Qi, Yi Pin; Nikonov, Sergey Y.; Niu, Li Na; Messer, Regina L W; Mao, Jing; Primus, Carolyn M.; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Journal of Endodontics, Vol. 38, No. 7, 01.07.2012, p. 936-942.

Research output: Contribution to journalArticle

Wei, Wei ; Qi, Yi Pin ; Nikonov, Sergey Y. ; Niu, Li Na ; Messer, Regina L W ; Mao, Jing ; Primus, Carolyn M. ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Effects of an experimental calcium aluminosilicate cement on the viability of murine odontoblast-like cells. In: Journal of Endodontics. 2012 ; Vol. 38, No. 7. pp. 936-942.
@article{9b47710182b84bc9af2476c005a53f24,
title = "Effects of an experimental calcium aluminosilicate cement on the viability of murine odontoblast-like cells",
abstract = "Introduction: Quick-setting calcium aluminosilicate cement with improved washout resistance is a potential substitute for calcium silicate cements in endodontics. This study examined the effect of an experimental calcium aluminosilicate cement (Quick-Set; Primus Consulting, Bradenton, FL) on the viability of odontoblast-like cells. Methods: The biocompatibility of Quick-Set and white ProRoot MTA (WMTA; Dentsply Tulsa Dental Specialties, Tulsa, OK) cements and their eluents was evaluated using a murine dental papilla-derived odontoblast-like cell line (MDPC-23); 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was used to examine the effects of the 2 hydraulic cements on mitochondrial metabolic activity. Flow cytometry and confocal laser scanning microscopy were used to identify the effects of the 2 cements on cell death-induced plasma membrane permeability to fluorescent dyes and DNA stains. Results: After the first week of immersion in culture medium, Quick-Set and WMTA were more cytotoxic than the Teflon-negative control (P <.05), and the cells exhibited more apoptosis/necrosis than Teflon (P <.05). After the second week of immersion, the 2 cements were as biocompatible as Teflon (P >.05), with cells exhibiting minimal apoptosis/necrosis. Eluents from the set cements at 1:1 dilution were significantly more cytotoxic that eluents at 1:10 or 1:100 dilution (P <.05). Conclusions: Quick-Set and WMTA exhibited similar cytotoxicity profiles. They possess negligible in vitro toxicologic risks after time-dependent elution of toxic components.",
keywords = "3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT assay, biocompatibility, calcium aluminate, flow cytometry, hydraulic cement, vital cell staining",
author = "Wei Wei and Qi, {Yi Pin} and Nikonov, {Sergey Y.} and Niu, {Li Na} and Messer, {Regina L W} and Jing Mao and Primus, {Carolyn M.} and Pashley, {David Henry} and Tay, {Franklin Chi Meng}",
year = "2012",
month = "7",
day = "1",
doi = "10.1016/j.joen.2012.03.020",
language = "English (US)",
volume = "38",
pages = "936--942",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier Inc.",
number = "7",

}

TY - JOUR

T1 - Effects of an experimental calcium aluminosilicate cement on the viability of murine odontoblast-like cells

AU - Wei, Wei

AU - Qi, Yi Pin

AU - Nikonov, Sergey Y.

AU - Niu, Li Na

AU - Messer, Regina L W

AU - Mao, Jing

AU - Primus, Carolyn M.

AU - Pashley, David Henry

AU - Tay, Franklin Chi Meng

PY - 2012/7/1

Y1 - 2012/7/1

N2 - Introduction: Quick-setting calcium aluminosilicate cement with improved washout resistance is a potential substitute for calcium silicate cements in endodontics. This study examined the effect of an experimental calcium aluminosilicate cement (Quick-Set; Primus Consulting, Bradenton, FL) on the viability of odontoblast-like cells. Methods: The biocompatibility of Quick-Set and white ProRoot MTA (WMTA; Dentsply Tulsa Dental Specialties, Tulsa, OK) cements and their eluents was evaluated using a murine dental papilla-derived odontoblast-like cell line (MDPC-23); 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was used to examine the effects of the 2 hydraulic cements on mitochondrial metabolic activity. Flow cytometry and confocal laser scanning microscopy were used to identify the effects of the 2 cements on cell death-induced plasma membrane permeability to fluorescent dyes and DNA stains. Results: After the first week of immersion in culture medium, Quick-Set and WMTA were more cytotoxic than the Teflon-negative control (P <.05), and the cells exhibited more apoptosis/necrosis than Teflon (P <.05). After the second week of immersion, the 2 cements were as biocompatible as Teflon (P >.05), with cells exhibiting minimal apoptosis/necrosis. Eluents from the set cements at 1:1 dilution were significantly more cytotoxic that eluents at 1:10 or 1:100 dilution (P <.05). Conclusions: Quick-Set and WMTA exhibited similar cytotoxicity profiles. They possess negligible in vitro toxicologic risks after time-dependent elution of toxic components.

AB - Introduction: Quick-setting calcium aluminosilicate cement with improved washout resistance is a potential substitute for calcium silicate cements in endodontics. This study examined the effect of an experimental calcium aluminosilicate cement (Quick-Set; Primus Consulting, Bradenton, FL) on the viability of odontoblast-like cells. Methods: The biocompatibility of Quick-Set and white ProRoot MTA (WMTA; Dentsply Tulsa Dental Specialties, Tulsa, OK) cements and their eluents was evaluated using a murine dental papilla-derived odontoblast-like cell line (MDPC-23); 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was used to examine the effects of the 2 hydraulic cements on mitochondrial metabolic activity. Flow cytometry and confocal laser scanning microscopy were used to identify the effects of the 2 cements on cell death-induced plasma membrane permeability to fluorescent dyes and DNA stains. Results: After the first week of immersion in culture medium, Quick-Set and WMTA were more cytotoxic than the Teflon-negative control (P <.05), and the cells exhibited more apoptosis/necrosis than Teflon (P <.05). After the second week of immersion, the 2 cements were as biocompatible as Teflon (P >.05), with cells exhibiting minimal apoptosis/necrosis. Eluents from the set cements at 1:1 dilution were significantly more cytotoxic that eluents at 1:10 or 1:100 dilution (P <.05). Conclusions: Quick-Set and WMTA exhibited similar cytotoxicity profiles. They possess negligible in vitro toxicologic risks after time-dependent elution of toxic components.

KW - 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

KW - MTT assay

KW - biocompatibility

KW - calcium aluminate

KW - flow cytometry

KW - hydraulic cement

KW - vital cell staining

UR - http://www.scopus.com/inward/record.url?scp=84862287670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862287670&partnerID=8YFLogxK

U2 - 10.1016/j.joen.2012.03.020

DO - 10.1016/j.joen.2012.03.020

M3 - Article

VL - 38

SP - 936

EP - 942

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 7

ER -