Effects of calcium silicate-based materials on collagen matrix integrity of mineralized dentin

Alaina P. Leiendecker, Yi Pin Qi, Allen N. Sawyer, Li Na Niu, Kelli A. Agee, Robert J. Loushine, Roger N. Weller, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Introduction: Calcium silicate-based materials (CSMs) are used in various endodontic procedures. The present study examined whether prolonged contact of mineralized dentin with recently commercialized versions of these materials adversely affects dentin collagen matrix integrity. Methods: Dentin slabs prepared from extracted human third molars (7 × 3 × 0.3 mm) were divided into 3 groups on the basis of the material to which dentin was exposed (MTA Plus, Biodentine, untreated control dentin slabs) and the time period of exposure (24 hours, 1, 2, and 3 months; n = 6). Hydroxyproline assay was performed on each group's supernatant to quantify the collagen extraction amounts of each group per time period. Data were analyzed with two-factor repeated-measures analysis of variance and Holm-Sidak pair-wise comparisons (α = 0.05) to determine the effects of material and aging time on collagen extraction. Dentin slabs from the 3 months of aging group were demineralized for transmission electron microscopy examination of collagen matrix ultrastructural changes. Results: Material (P =.002), aging time (P <.001), and their interactions (P =.007) significantly affected the amount of hydroxyproline (pg/mg of mineralized dentin) extracted from mineralized dentin and were significantly correlated by power regression models. Collagen degradation was identified from the surface of dentin slabs that were in direct contact with CSMs. Conclusions: Prolonged contact of mineralized dentin with CSMs has an adverse effect on the integrity of the dentin collagen matrix. However, the amount of collagen extracted was limited to the contact surface. Clinicians can continue to apply CSMs in endodontic procedures; however, caution is advised when these materials are applied to thin dentinal walls.

Original languageEnglish (US)
Pages (from-to)829-833
Number of pages5
JournalJournal of Endodontics
Volume38
Issue number6
DOIs
StatePublished - Jun 1 2012

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Dentin
Collagen
Endodontics
Hydroxyproline
Pemetrexed
calcium silicate
Third Molar
Transmission Electron Microscopy
Analysis of Variance

Keywords

  • Calcium silicate
  • collagen
  • hydroxyproline
  • transmission electron microscopy

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effects of calcium silicate-based materials on collagen matrix integrity of mineralized dentin. / Leiendecker, Alaina P.; Qi, Yi Pin; Sawyer, Allen N.; Niu, Li Na; Agee, Kelli A.; Loushine, Robert J.; Weller, Roger N.; Pashley, David H.; Tay, Franklin R.

In: Journal of Endodontics, Vol. 38, No. 6, 01.06.2012, p. 829-833.

Research output: Contribution to journalArticle

Leiendecker, AP, Qi, YP, Sawyer, AN, Niu, LN, Agee, KA, Loushine, RJ, Weller, RN, Pashley, DH & Tay, FR 2012, 'Effects of calcium silicate-based materials on collagen matrix integrity of mineralized dentin', Journal of Endodontics, vol. 38, no. 6, pp. 829-833. https://doi.org/10.1016/j.joen.2012.01.004
Leiendecker, Alaina P. ; Qi, Yi Pin ; Sawyer, Allen N. ; Niu, Li Na ; Agee, Kelli A. ; Loushine, Robert J. ; Weller, Roger N. ; Pashley, David H. ; Tay, Franklin R. / Effects of calcium silicate-based materials on collagen matrix integrity of mineralized dentin. In: Journal of Endodontics. 2012 ; Vol. 38, No. 6. pp. 829-833.
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abstract = "Introduction: Calcium silicate-based materials (CSMs) are used in various endodontic procedures. The present study examined whether prolonged contact of mineralized dentin with recently commercialized versions of these materials adversely affects dentin collagen matrix integrity. Methods: Dentin slabs prepared from extracted human third molars (7 × 3 × 0.3 mm) were divided into 3 groups on the basis of the material to which dentin was exposed (MTA Plus, Biodentine, untreated control dentin slabs) and the time period of exposure (24 hours, 1, 2, and 3 months; n = 6). Hydroxyproline assay was performed on each group's supernatant to quantify the collagen extraction amounts of each group per time period. Data were analyzed with two-factor repeated-measures analysis of variance and Holm-Sidak pair-wise comparisons (α = 0.05) to determine the effects of material and aging time on collagen extraction. Dentin slabs from the 3 months of aging group were demineralized for transmission electron microscopy examination of collagen matrix ultrastructural changes. Results: Material (P =.002), aging time (P <.001), and their interactions (P =.007) significantly affected the amount of hydroxyproline (pg/mg of mineralized dentin) extracted from mineralized dentin and were significantly correlated by power regression models. Collagen degradation was identified from the surface of dentin slabs that were in direct contact with CSMs. Conclusions: Prolonged contact of mineralized dentin with CSMs has an adverse effect on the integrity of the dentin collagen matrix. However, the amount of collagen extracted was limited to the contact surface. Clinicians can continue to apply CSMs in endodontic procedures; however, caution is advised when these materials are applied to thin dentinal walls.",
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AU - Leiendecker, Alaina P.

AU - Qi, Yi Pin

AU - Sawyer, Allen N.

AU - Niu, Li Na

AU - Agee, Kelli A.

AU - Loushine, Robert J.

AU - Weller, Roger N.

AU - Pashley, David H.

AU - Tay, Franklin R.

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N2 - Introduction: Calcium silicate-based materials (CSMs) are used in various endodontic procedures. The present study examined whether prolonged contact of mineralized dentin with recently commercialized versions of these materials adversely affects dentin collagen matrix integrity. Methods: Dentin slabs prepared from extracted human third molars (7 × 3 × 0.3 mm) were divided into 3 groups on the basis of the material to which dentin was exposed (MTA Plus, Biodentine, untreated control dentin slabs) and the time period of exposure (24 hours, 1, 2, and 3 months; n = 6). Hydroxyproline assay was performed on each group's supernatant to quantify the collagen extraction amounts of each group per time period. Data were analyzed with two-factor repeated-measures analysis of variance and Holm-Sidak pair-wise comparisons (α = 0.05) to determine the effects of material and aging time on collagen extraction. Dentin slabs from the 3 months of aging group were demineralized for transmission electron microscopy examination of collagen matrix ultrastructural changes. Results: Material (P =.002), aging time (P <.001), and their interactions (P =.007) significantly affected the amount of hydroxyproline (pg/mg of mineralized dentin) extracted from mineralized dentin and were significantly correlated by power regression models. Collagen degradation was identified from the surface of dentin slabs that were in direct contact with CSMs. Conclusions: Prolonged contact of mineralized dentin with CSMs has an adverse effect on the integrity of the dentin collagen matrix. However, the amount of collagen extracted was limited to the contact surface. Clinicians can continue to apply CSMs in endodontic procedures; however, caution is advised when these materials are applied to thin dentinal walls.

AB - Introduction: Calcium silicate-based materials (CSMs) are used in various endodontic procedures. The present study examined whether prolonged contact of mineralized dentin with recently commercialized versions of these materials adversely affects dentin collagen matrix integrity. Methods: Dentin slabs prepared from extracted human third molars (7 × 3 × 0.3 mm) were divided into 3 groups on the basis of the material to which dentin was exposed (MTA Plus, Biodentine, untreated control dentin slabs) and the time period of exposure (24 hours, 1, 2, and 3 months; n = 6). Hydroxyproline assay was performed on each group's supernatant to quantify the collagen extraction amounts of each group per time period. Data were analyzed with two-factor repeated-measures analysis of variance and Holm-Sidak pair-wise comparisons (α = 0.05) to determine the effects of material and aging time on collagen extraction. Dentin slabs from the 3 months of aging group were demineralized for transmission electron microscopy examination of collagen matrix ultrastructural changes. Results: Material (P =.002), aging time (P <.001), and their interactions (P =.007) significantly affected the amount of hydroxyproline (pg/mg of mineralized dentin) extracted from mineralized dentin and were significantly correlated by power regression models. Collagen degradation was identified from the surface of dentin slabs that were in direct contact with CSMs. Conclusions: Prolonged contact of mineralized dentin with CSMs has an adverse effect on the integrity of the dentin collagen matrix. However, the amount of collagen extracted was limited to the contact surface. Clinicians can continue to apply CSMs in endodontic procedures; however, caution is advised when these materials are applied to thin dentinal walls.

KW - Calcium silicate

KW - collagen

KW - hydroxyproline

KW - transmission electron microscopy

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