Effects of Different Exposure Times and Concentrations of Sodium Hypochlorite/Ethylenediaminetetraacetic Acid on the Structural Integrity of Mineralized Dentin

Kai Zhang, Young Kyung Kim, Milena Cadenaro, Thomas E. Bryan, Stephanie Jane Sidow, Robert J. Loushine, Jun qi Ling, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Introduction: This study tested the null hypothesis that there is no difference between the use of 1.3% NaOCl/17% ethylenediaminetetraacetic acid (EDTA) and 5.25% NaOCl/17% EDTA irrigation regimens on the collagen degradation and flexural strength reduction in mineralized dentin. Methods: Dentin powder and mineralized dentin sections were immersed in 1.3% or 5.25% NaOCl for 10-240 minutes and then rinsed with 17% EDTA as the final irrigant for 2 minutes. Untreated mineralized dentin powder/sections served as controls in the respective experiments. Dentin powders were examined by using Fourier transform infrared (FT-IR) spectroscopy to analyze their relative subsurface intact collagen content with the apatite/collagen ratio. Hydrated dentin sections were subjected to 3-point flexure under water for determining their flexural strengths. Results: Collagen degradation was significantly increased and the flexural strength of mineralized dentin was significantly reduced after the use of 5.25% NaOCl as the initial irrigant for more than 1 hour (P < .05). Conversely, changes were insignificant when 1.3% NaOCl was used as the initial irrigant for up to 4 hours (Kruskal-Wallis analysis of variance, n = 10, P < .05). Conclusions: The null hypothesis was rejected. The deleterious effects attributed to the use of NaOCl on dentin are concentration-dependent and time-dependent and are not associated with the demineralization caused by the use of EDTA as the final active irrigant.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalJournal of Endodontics
Volume36
Issue number1
DOIs
StatePublished - Jan 1 2010

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Sodium Hypochlorite
Dentin
Edetic Acid
Collagen
Powders
Apatites
Fourier Transform Infrared Spectroscopy
Analysis of Variance
Water

Keywords

  • Collagen
  • Fourier transform infrared spectroscopy
  • denaturation
  • ethylenediamine tetraacetic acid
  • flexural strength
  • irrigation
  • sodium hypochlorite
  • three point bending

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effects of Different Exposure Times and Concentrations of Sodium Hypochlorite/Ethylenediaminetetraacetic Acid on the Structural Integrity of Mineralized Dentin. / Zhang, Kai; Kim, Young Kyung; Cadenaro, Milena; Bryan, Thomas E.; Sidow, Stephanie Jane; Loushine, Robert J.; Ling, Jun qi; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Journal of Endodontics, Vol. 36, No. 1, 01.01.2010, p. 105-109.

Research output: Contribution to journalArticle

Zhang, Kai ; Kim, Young Kyung ; Cadenaro, Milena ; Bryan, Thomas E. ; Sidow, Stephanie Jane ; Loushine, Robert J. ; Ling, Jun qi ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Effects of Different Exposure Times and Concentrations of Sodium Hypochlorite/Ethylenediaminetetraacetic Acid on the Structural Integrity of Mineralized Dentin. In: Journal of Endodontics. 2010 ; Vol. 36, No. 1. pp. 105-109.
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abstract = "Introduction: This study tested the null hypothesis that there is no difference between the use of 1.3{\%} NaOCl/17{\%} ethylenediaminetetraacetic acid (EDTA) and 5.25{\%} NaOCl/17{\%} EDTA irrigation regimens on the collagen degradation and flexural strength reduction in mineralized dentin. Methods: Dentin powder and mineralized dentin sections were immersed in 1.3{\%} or 5.25{\%} NaOCl for 10-240 minutes and then rinsed with 17{\%} EDTA as the final irrigant for 2 minutes. Untreated mineralized dentin powder/sections served as controls in the respective experiments. Dentin powders were examined by using Fourier transform infrared (FT-IR) spectroscopy to analyze their relative subsurface intact collagen content with the apatite/collagen ratio. Hydrated dentin sections were subjected to 3-point flexure under water for determining their flexural strengths. Results: Collagen degradation was significantly increased and the flexural strength of mineralized dentin was significantly reduced after the use of 5.25{\%} NaOCl as the initial irrigant for more than 1 hour (P < .05). Conversely, changes were insignificant when 1.3{\%} NaOCl was used as the initial irrigant for up to 4 hours (Kruskal-Wallis analysis of variance, n = 10, P < .05). Conclusions: The null hypothesis was rejected. The deleterious effects attributed to the use of NaOCl on dentin are concentration-dependent and time-dependent and are not associated with the demineralization caused by the use of EDTA as the final active irrigant.",
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T1 - Effects of Different Exposure Times and Concentrations of Sodium Hypochlorite/Ethylenediaminetetraacetic Acid on the Structural Integrity of Mineralized Dentin

AU - Zhang, Kai

AU - Kim, Young Kyung

AU - Cadenaro, Milena

AU - Bryan, Thomas E.

AU - Sidow, Stephanie Jane

AU - Loushine, Robert J.

AU - Ling, Jun qi

AU - Pashley, David Henry

AU - Tay, Franklin Chi Meng

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Introduction: This study tested the null hypothesis that there is no difference between the use of 1.3% NaOCl/17% ethylenediaminetetraacetic acid (EDTA) and 5.25% NaOCl/17% EDTA irrigation regimens on the collagen degradation and flexural strength reduction in mineralized dentin. Methods: Dentin powder and mineralized dentin sections were immersed in 1.3% or 5.25% NaOCl for 10-240 minutes and then rinsed with 17% EDTA as the final irrigant for 2 minutes. Untreated mineralized dentin powder/sections served as controls in the respective experiments. Dentin powders were examined by using Fourier transform infrared (FT-IR) spectroscopy to analyze their relative subsurface intact collagen content with the apatite/collagen ratio. Hydrated dentin sections were subjected to 3-point flexure under water for determining their flexural strengths. Results: Collagen degradation was significantly increased and the flexural strength of mineralized dentin was significantly reduced after the use of 5.25% NaOCl as the initial irrigant for more than 1 hour (P < .05). Conversely, changes were insignificant when 1.3% NaOCl was used as the initial irrigant for up to 4 hours (Kruskal-Wallis analysis of variance, n = 10, P < .05). Conclusions: The null hypothesis was rejected. The deleterious effects attributed to the use of NaOCl on dentin are concentration-dependent and time-dependent and are not associated with the demineralization caused by the use of EDTA as the final active irrigant.

AB - Introduction: This study tested the null hypothesis that there is no difference between the use of 1.3% NaOCl/17% ethylenediaminetetraacetic acid (EDTA) and 5.25% NaOCl/17% EDTA irrigation regimens on the collagen degradation and flexural strength reduction in mineralized dentin. Methods: Dentin powder and mineralized dentin sections were immersed in 1.3% or 5.25% NaOCl for 10-240 minutes and then rinsed with 17% EDTA as the final irrigant for 2 minutes. Untreated mineralized dentin powder/sections served as controls in the respective experiments. Dentin powders were examined by using Fourier transform infrared (FT-IR) spectroscopy to analyze their relative subsurface intact collagen content with the apatite/collagen ratio. Hydrated dentin sections were subjected to 3-point flexure under water for determining their flexural strengths. Results: Collagen degradation was significantly increased and the flexural strength of mineralized dentin was significantly reduced after the use of 5.25% NaOCl as the initial irrigant for more than 1 hour (P < .05). Conversely, changes were insignificant when 1.3% NaOCl was used as the initial irrigant for up to 4 hours (Kruskal-Wallis analysis of variance, n = 10, P < .05). Conclusions: The null hypothesis was rejected. The deleterious effects attributed to the use of NaOCl on dentin are concentration-dependent and time-dependent and are not associated with the demineralization caused by the use of EDTA as the final active irrigant.

KW - Collagen

KW - Fourier transform infrared spectroscopy

KW - denaturation

KW - ethylenediamine tetraacetic acid

KW - flexural strength

KW - irrigation

KW - sodium hypochlorite

KW - three point bending

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