Effects of EDC crosslinking on the stiffness of dentin hybrid layers evaluated by nanoDMA over time

Zihou Zhang, Mustafa Mutluay, Arzu Tezvergil-Mutluay, Franklin Chi Meng Tay, David Henry Pashley, Dwayne Arola

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Application of collagen cross-linkers to demineralized dentin improves bond durability. While the benefits of cross-linking treatments to bond strength and fatigue resistance have been explored, changes in hybrid layer stiffness with aging have not been examined. Objective To examine the influence of a cross-linking treatment using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) on hybrid layer stiffness of resin-dentin adhesive bonds, using spatially-resolved nanoscopic Dynamic Mechanical Analysis (nanoDMA). Methods Bonded interface specimens were prepared using a two-step (SB) or three-step (SBMP) etch-and-rinse adhesive. Adhesive bonding of the treated groups was preceded by a 1 min application of an experimental EDC conditioner to the acid-etched dentin. Control specimens did not receive EDC treatment. The bonded interfaces were evaluated using nanoDMA to determine the dynamic mechanical properties after storage in artificial saliva at 37 °C for 0, 3 and 6 months. Results The EDC treatment had no influence on the dynamic mechanical properties of the hybrid layer immediately after bonding. There was also no reduction in the hybrid layer stiffness after 3 and 6 months aging as defined by the complex modulus and storage modulus. However, there was a significant reduction in the loss modulus and tan δ components (i.e. viscous behavior) of the hybrid layers with aging. Degradation occurred to both adhesive systems with storage, but was greatest for SB. Without EDC treatment, the reduction in tan δ of the hybrid layer prepared with SB exceeded 80% in 6 months. Significance The application of EDC to acid-etched dentin helps maintain the viscoelasticity of hybrid layers.

Original languageEnglish (US)
Pages (from-to)904-914
Number of pages11
JournalDental Materials
Volume33
Issue number8
DOIs
StatePublished - Aug 1 2017

Fingerprint

Dynamic mechanical analysis
Dentin
Adhesives
Crosslinking
Stiffness
Aging of materials
Ethyldimethylaminopropyl Carbodiimide
Artificial Saliva
Mechanical properties
Acids
Viscoelasticity
Collagen
Fatigue
Durability
Resins
Elastic moduli
Fatigue of materials
Degradation

Keywords

  • Collagen
  • Cross-linker
  • Dentin bonding agents
  • Durability
  • EDC
  • Endogenous proteinases
  • Viscoelasticity

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Effects of EDC crosslinking on the stiffness of dentin hybrid layers evaluated by nanoDMA over time. / Zhang, Zihou; Mutluay, Mustafa; Tezvergil-Mutluay, Arzu; Tay, Franklin Chi Meng; Pashley, David Henry; Arola, Dwayne.

In: Dental Materials, Vol. 33, No. 8, 01.08.2017, p. 904-914.

Research output: Contribution to journalArticle

Zhang, Zihou ; Mutluay, Mustafa ; Tezvergil-Mutluay, Arzu ; Tay, Franklin Chi Meng ; Pashley, David Henry ; Arola, Dwayne. / Effects of EDC crosslinking on the stiffness of dentin hybrid layers evaluated by nanoDMA over time. In: Dental Materials. 2017 ; Vol. 33, No. 8. pp. 904-914.
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abstract = "Application of collagen cross-linkers to demineralized dentin improves bond durability. While the benefits of cross-linking treatments to bond strength and fatigue resistance have been explored, changes in hybrid layer stiffness with aging have not been examined. Objective To examine the influence of a cross-linking treatment using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) on hybrid layer stiffness of resin-dentin adhesive bonds, using spatially-resolved nanoscopic Dynamic Mechanical Analysis (nanoDMA). Methods Bonded interface specimens were prepared using a two-step (SB) or three-step (SBMP) etch-and-rinse adhesive. Adhesive bonding of the treated groups was preceded by a 1 min application of an experimental EDC conditioner to the acid-etched dentin. Control specimens did not receive EDC treatment. The bonded interfaces were evaluated using nanoDMA to determine the dynamic mechanical properties after storage in artificial saliva at 37 °C for 0, 3 and 6 months. Results The EDC treatment had no influence on the dynamic mechanical properties of the hybrid layer immediately after bonding. There was also no reduction in the hybrid layer stiffness after 3 and 6 months aging as defined by the complex modulus and storage modulus. However, there was a significant reduction in the loss modulus and tan δ components (i.e. viscous behavior) of the hybrid layers with aging. Degradation occurred to both adhesive systems with storage, but was greatest for SB. Without EDC treatment, the reduction in tan δ of the hybrid layer prepared with SB exceeded 80{\%} in 6 months. Significance The application of EDC to acid-etched dentin helps maintain the viscoelasticity of hybrid layers.",
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AU - Pashley, David Henry

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N2 - Application of collagen cross-linkers to demineralized dentin improves bond durability. While the benefits of cross-linking treatments to bond strength and fatigue resistance have been explored, changes in hybrid layer stiffness with aging have not been examined. Objective To examine the influence of a cross-linking treatment using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) on hybrid layer stiffness of resin-dentin adhesive bonds, using spatially-resolved nanoscopic Dynamic Mechanical Analysis (nanoDMA). Methods Bonded interface specimens were prepared using a two-step (SB) or three-step (SBMP) etch-and-rinse adhesive. Adhesive bonding of the treated groups was preceded by a 1 min application of an experimental EDC conditioner to the acid-etched dentin. Control specimens did not receive EDC treatment. The bonded interfaces were evaluated using nanoDMA to determine the dynamic mechanical properties after storage in artificial saliva at 37 °C for 0, 3 and 6 months. Results The EDC treatment had no influence on the dynamic mechanical properties of the hybrid layer immediately after bonding. There was also no reduction in the hybrid layer stiffness after 3 and 6 months aging as defined by the complex modulus and storage modulus. However, there was a significant reduction in the loss modulus and tan δ components (i.e. viscous behavior) of the hybrid layers with aging. Degradation occurred to both adhesive systems with storage, but was greatest for SB. Without EDC treatment, the reduction in tan δ of the hybrid layer prepared with SB exceeded 80% in 6 months. Significance The application of EDC to acid-etched dentin helps maintain the viscoelasticity of hybrid layers.

AB - Application of collagen cross-linkers to demineralized dentin improves bond durability. While the benefits of cross-linking treatments to bond strength and fatigue resistance have been explored, changes in hybrid layer stiffness with aging have not been examined. Objective To examine the influence of a cross-linking treatment using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) on hybrid layer stiffness of resin-dentin adhesive bonds, using spatially-resolved nanoscopic Dynamic Mechanical Analysis (nanoDMA). Methods Bonded interface specimens were prepared using a two-step (SB) or three-step (SBMP) etch-and-rinse adhesive. Adhesive bonding of the treated groups was preceded by a 1 min application of an experimental EDC conditioner to the acid-etched dentin. Control specimens did not receive EDC treatment. The bonded interfaces were evaluated using nanoDMA to determine the dynamic mechanical properties after storage in artificial saliva at 37 °C for 0, 3 and 6 months. Results The EDC treatment had no influence on the dynamic mechanical properties of the hybrid layer immediately after bonding. There was also no reduction in the hybrid layer stiffness after 3 and 6 months aging as defined by the complex modulus and storage modulus. However, there was a significant reduction in the loss modulus and tan δ components (i.e. viscous behavior) of the hybrid layers with aging. Degradation occurred to both adhesive systems with storage, but was greatest for SB. Without EDC treatment, the reduction in tan δ of the hybrid layer prepared with SB exceeded 80% in 6 months. Significance The application of EDC to acid-etched dentin helps maintain the viscoelasticity of hybrid layers.

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KW - Endogenous proteinases

KW - Viscoelasticity

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