Effects of endotoxin on neutrophil-mediated ischemia/reperfusion injury in the rat heart in vivo

Jun Zhou, Ming Xia Shi, Tiffany D. Mitchell, Gennady N. Smagin, Sonyja R. Thomas, Donna H. Ryan, Ruth Babette Harris

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have previously shown that a nonlethal dose of lipopolysaccharide (LPS) decreases L-selectin expression of neutrophils (PMNs), thereby preventing PMN-mediated reperfusion injury in the isolated heart. In the present study we determined whether or not that dose of LPS would protect hearts during in vivo ischemia and reperfusion by preventing PMN-induced reperfusion injury. Rats receiving saline vehicle showed marked myocardial injury (necrotic area/area at risk = 82% ± 2%) and significant depression in left ventricular function as assessed in the isolated isovolumic heart preparation at constant flow rates of 5, 10, 15, and 20 ml/min. The administration of LPS (100 μg/kg body wt) 7 hr prior to ischemia resulted in a reduction in myocardial damage (necrotic area/area at risk = 42% ± 3%) and preservation of function. Myocardial function was similar to that of sham ischemic saline- and LPS-treated rats. Moreover, PMN infiltration as determined by histology was quantitatively more severe in hearts of saline-treated rats than in hearts of LPS-treated rats. Isolated hearts from vehicle- and LPS-treated animals undergoing sham ischemia in vivo recovered to the same extent after in vitro ischemia/reperfusion, suggesting that LPS did not induce protection by altering intrinsic properties of the heart. Our results indicate that LPS-induced protection of the heart from in vivo PMN-mediated ischemia/reperfusion injury may be due to decreased L-selectin expression of PMNs in LPS-treated animals.

Original languageEnglish (US)
Pages (from-to)320-327
Number of pages8
JournalExperimental Biology and Medicine
Volume226
Issue number4
StatePublished - Jun 21 2001
Externally publishedYes

Fingerprint

Reperfusion Injury
Endotoxins
Lipopolysaccharides
Rats
Neutrophils
Ischemia
L-Selectin
Reperfusion
Animals
Histology
Left Ventricular Function
Infiltration
Flow rate
Wounds and Injuries

Keywords

  • Endotoxin
  • L-selectin
  • LPS
  • Myocardium
  • Rat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Zhou, J., Shi, M. X., Mitchell, T. D., Smagin, G. N., Thomas, S. R., Ryan, D. H., & Harris, R. B. (2001). Effects of endotoxin on neutrophil-mediated ischemia/reperfusion injury in the rat heart in vivo. Experimental Biology and Medicine, 226(4), 320-327.

Effects of endotoxin on neutrophil-mediated ischemia/reperfusion injury in the rat heart in vivo. / Zhou, Jun; Shi, Ming Xia; Mitchell, Tiffany D.; Smagin, Gennady N.; Thomas, Sonyja R.; Ryan, Donna H.; Harris, Ruth Babette.

In: Experimental Biology and Medicine, Vol. 226, No. 4, 21.06.2001, p. 320-327.

Research output: Contribution to journalArticle

Zhou, J, Shi, MX, Mitchell, TD, Smagin, GN, Thomas, SR, Ryan, DH & Harris, RB 2001, 'Effects of endotoxin on neutrophil-mediated ischemia/reperfusion injury in the rat heart in vivo', Experimental Biology and Medicine, vol. 226, no. 4, pp. 320-327.
Zhou J, Shi MX, Mitchell TD, Smagin GN, Thomas SR, Ryan DH et al. Effects of endotoxin on neutrophil-mediated ischemia/reperfusion injury in the rat heart in vivo. Experimental Biology and Medicine. 2001 Jun 21;226(4):320-327.
Zhou, Jun ; Shi, Ming Xia ; Mitchell, Tiffany D. ; Smagin, Gennady N. ; Thomas, Sonyja R. ; Ryan, Donna H. ; Harris, Ruth Babette. / Effects of endotoxin on neutrophil-mediated ischemia/reperfusion injury in the rat heart in vivo. In: Experimental Biology and Medicine. 2001 ; Vol. 226, No. 4. pp. 320-327.
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