Effects of exogenous collagenase and cholesterol esterase on the durability of the resin-dentin bond

Steve R. Armstrong, Julie L.P. Jessop, Marcos A. Vargas, Yuan Zou, Fang Qian, Joshua A. Campbell, David Henry Pashley

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Purpose: The purpose of this work was to determine microtensile dentin bond strengths (μTBS) of dentin-resin composite bonds after three-month storage in artificial saliva containing either collagenase (COL) or cholesterol esterase (EST). The null hypothesis tested is that the resin-dentin bond strength is equivalent for each storage medium at the tested storage times. Materials and Methods: Resin composite was bonded to occlusal dentin, and μTBS specimens were formed and stored in the artificial saliva, COL, EST, or synthetic oil. After 24 h and 12-week storage, μTBS was determined and failure modes were characterized by SEM. The interfacial ultrastructure was evaluated by transmission electron microscopy as unstained and stained sections (phosphotungstic acid/uranyl acetate). Statistical analysis was performed by ANOVA and Weibull survival analyses at the 0.05 level of statistical significance. Results: There were significantly weaker bond strengths after 12 weeks for all experimental storage media (p < 0.001). Artificial saliva containing EST lowered bond strengths to a significantly greater extent than did COL after 12 weeks of storage, while no difference between these groups could be discerned after 24 h. Therefore, the null hypothesis of this experiment is rejected. Conclusion: Exogenous enzymatic challenge to resin-dentin bonds decreased bond durability only with EST. However, when further challenges to ideal infiltration of the comonomers into the hybrid layer were carried out using inadequate removal of solvent, additional alterations in hybrid layer ultrastructure were discerned by TEM that may represent different potential degradative processes. The contribution of endogenous enzymatic challenges to the primary degradative process, ie, hydrolysis, is unknown and deserves continued attention.

Original languageEnglish (US)
Pages (from-to)151-160
Number of pages10
JournalJournal of Adhesive Dentistry
Volume8
Issue number3
StatePublished - Jul 13 2006

Fingerprint

Sterol Esterase
Collagenases
Dentin
Artificial Saliva
Esterases
Composite Resins
Phosphotungstic Acid
Survival Analysis
Transmission Electron Microscopy
Analysis of Variance
Oils
Hydrolysis

Keywords

  • Degradation
  • Dental adhesives
  • Dentin
  • Enzymes
  • Hydrolysis

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Periodontics

Cite this

Armstrong, S. R., Jessop, J. L. P., Vargas, M. A., Zou, Y., Qian, F., Campbell, J. A., & Pashley, D. H. (2006). Effects of exogenous collagenase and cholesterol esterase on the durability of the resin-dentin bond. Journal of Adhesive Dentistry, 8(3), 151-160.

Effects of exogenous collagenase and cholesterol esterase on the durability of the resin-dentin bond. / Armstrong, Steve R.; Jessop, Julie L.P.; Vargas, Marcos A.; Zou, Yuan; Qian, Fang; Campbell, Joshua A.; Pashley, David Henry.

In: Journal of Adhesive Dentistry, Vol. 8, No. 3, 13.07.2006, p. 151-160.

Research output: Contribution to journalArticle

Armstrong, SR, Jessop, JLP, Vargas, MA, Zou, Y, Qian, F, Campbell, JA & Pashley, DH 2006, 'Effects of exogenous collagenase and cholesterol esterase on the durability of the resin-dentin bond', Journal of Adhesive Dentistry, vol. 8, no. 3, pp. 151-160.
Armstrong SR, Jessop JLP, Vargas MA, Zou Y, Qian F, Campbell JA et al. Effects of exogenous collagenase and cholesterol esterase on the durability of the resin-dentin bond. Journal of Adhesive Dentistry. 2006 Jul 13;8(3):151-160.
Armstrong, Steve R. ; Jessop, Julie L.P. ; Vargas, Marcos A. ; Zou, Yuan ; Qian, Fang ; Campbell, Joshua A. ; Pashley, David Henry. / Effects of exogenous collagenase and cholesterol esterase on the durability of the resin-dentin bond. In: Journal of Adhesive Dentistry. 2006 ; Vol. 8, No. 3. pp. 151-160.
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AU - Qian, Fang

AU - Campbell, Joshua A.

AU - Pashley, David Henry

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