Effects of genistein on experimental metastasis of B16-BL6 mouse melanoma cells

Chunhong Yan, Rui Han

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

AIM: To investigate the effects of genistein, an isoflavone, on mouse experimental metastasis in order to explore the possibility of developing genistein as a novel anti-cancer drug. METHODS: B16-BL6 mouse melanoma cells were injected into C57BL/6 mouse via tail lateral vein, which subsequently colonized into the animal lungs to form a large amount of pulmonary metastases after 15 days. Genistein was suspended in 2% lecithin and administered at 100 or 200 mg•kg-1 • d-1 by intraperitoneal injection daily from the day before the cell injection. RESULTS: The mean number of metastases in the animal group that were given genistein 200 mg•kg-1 was significantly reduced as compared with that of the control group(P<0.01), suggesting the anti-metastasis effect of genistein. n contrast, cyclophosphamide 100 mg • kg-1 ip did not significantly decrease the number of pulmonary metastases, while it was found to dramatically reduce the size of metastases. When genistein was administrated with cyclophosphamide, the survival time of the metastatic mice was significantly prolonged. CONCLUSION: Genistein inhibited experimental metastasis through a mechanism different from that of cyclophosphamide. The stronger life-prolonging effect of co-administration of genistein with cyclophosphamide suggests that it may be valuable to combine genistein with another cytotoxic drug for cancer metastasis chemotherapy.

Original languageEnglish (US)
Pages (from-to)814-817
Number of pages4
JournalYaoxue Xuebao
Volume34
Issue number11
StatePublished - Nov 1 1999

Fingerprint

Genistein
Melanoma
Neoplasm Metastasis
Cyclophosphamide
Lung
Isoflavones
Lecithins
Intraperitoneal Injections
Inbred C57BL Mouse
Pharmaceutical Preparations
Tail
Veins
Neoplasms
Drug Therapy
Control Groups
Injections

Keywords

  • Anti-tumor drug
  • Genistein
  • Mouse melanoma
  • Tumor metastasis

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Effects of genistein on experimental metastasis of B16-BL6 mouse melanoma cells. / Yan, Chunhong; Han, Rui.

In: Yaoxue Xuebao, Vol. 34, No. 11, 01.11.1999, p. 814-817.

Research output: Contribution to journalArticle

@article{6d8b500a1d49439982216953d9b07155,
title = "Effects of genistein on experimental metastasis of B16-BL6 mouse melanoma cells",
abstract = "AIM: To investigate the effects of genistein, an isoflavone, on mouse experimental metastasis in order to explore the possibility of developing genistein as a novel anti-cancer drug. METHODS: B16-BL6 mouse melanoma cells were injected into C57BL/6 mouse via tail lateral vein, which subsequently colonized into the animal lungs to form a large amount of pulmonary metastases after 15 days. Genistein was suspended in 2{\%} lecithin and administered at 100 or 200 mg•kg-1 • d-1 by intraperitoneal injection daily from the day before the cell injection. RESULTS: The mean number of metastases in the animal group that were given genistein 200 mg•kg-1 was significantly reduced as compared with that of the control group(P<0.01), suggesting the anti-metastasis effect of genistein. n contrast, cyclophosphamide 100 mg • kg-1 ip did not significantly decrease the number of pulmonary metastases, while it was found to dramatically reduce the size of metastases. When genistein was administrated with cyclophosphamide, the survival time of the metastatic mice was significantly prolonged. CONCLUSION: Genistein inhibited experimental metastasis through a mechanism different from that of cyclophosphamide. The stronger life-prolonging effect of co-administration of genistein with cyclophosphamide suggests that it may be valuable to combine genistein with another cytotoxic drug for cancer metastasis chemotherapy.",
keywords = "Anti-tumor drug, Genistein, Mouse melanoma, Tumor metastasis",
author = "Chunhong Yan and Rui Han",
year = "1999",
month = "11",
day = "1",
language = "English (US)",
volume = "34",
pages = "814--817",
journal = "Yao xue xue bao = Acta pharmaceutica Sinica",
issn = "0513-4870",
publisher = "Zhonghua Yixuehui Zazhishe",
number = "11",

}

TY - JOUR

T1 - Effects of genistein on experimental metastasis of B16-BL6 mouse melanoma cells

AU - Yan, Chunhong

AU - Han, Rui

PY - 1999/11/1

Y1 - 1999/11/1

N2 - AIM: To investigate the effects of genistein, an isoflavone, on mouse experimental metastasis in order to explore the possibility of developing genistein as a novel anti-cancer drug. METHODS: B16-BL6 mouse melanoma cells were injected into C57BL/6 mouse via tail lateral vein, which subsequently colonized into the animal lungs to form a large amount of pulmonary metastases after 15 days. Genistein was suspended in 2% lecithin and administered at 100 or 200 mg•kg-1 • d-1 by intraperitoneal injection daily from the day before the cell injection. RESULTS: The mean number of metastases in the animal group that were given genistein 200 mg•kg-1 was significantly reduced as compared with that of the control group(P<0.01), suggesting the anti-metastasis effect of genistein. n contrast, cyclophosphamide 100 mg • kg-1 ip did not significantly decrease the number of pulmonary metastases, while it was found to dramatically reduce the size of metastases. When genistein was administrated with cyclophosphamide, the survival time of the metastatic mice was significantly prolonged. CONCLUSION: Genistein inhibited experimental metastasis through a mechanism different from that of cyclophosphamide. The stronger life-prolonging effect of co-administration of genistein with cyclophosphamide suggests that it may be valuable to combine genistein with another cytotoxic drug for cancer metastasis chemotherapy.

AB - AIM: To investigate the effects of genistein, an isoflavone, on mouse experimental metastasis in order to explore the possibility of developing genistein as a novel anti-cancer drug. METHODS: B16-BL6 mouse melanoma cells were injected into C57BL/6 mouse via tail lateral vein, which subsequently colonized into the animal lungs to form a large amount of pulmonary metastases after 15 days. Genistein was suspended in 2% lecithin and administered at 100 or 200 mg•kg-1 • d-1 by intraperitoneal injection daily from the day before the cell injection. RESULTS: The mean number of metastases in the animal group that were given genistein 200 mg•kg-1 was significantly reduced as compared with that of the control group(P<0.01), suggesting the anti-metastasis effect of genistein. n contrast, cyclophosphamide 100 mg • kg-1 ip did not significantly decrease the number of pulmonary metastases, while it was found to dramatically reduce the size of metastases. When genistein was administrated with cyclophosphamide, the survival time of the metastatic mice was significantly prolonged. CONCLUSION: Genistein inhibited experimental metastasis through a mechanism different from that of cyclophosphamide. The stronger life-prolonging effect of co-administration of genistein with cyclophosphamide suggests that it may be valuable to combine genistein with another cytotoxic drug for cancer metastasis chemotherapy.

KW - Anti-tumor drug

KW - Genistein

KW - Mouse melanoma

KW - Tumor metastasis

UR - http://www.scopus.com/inward/record.url?scp=0041722505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041722505&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0041722505

VL - 34

SP - 814

EP - 817

JO - Yao xue xue bao = Acta pharmaceutica Sinica

JF - Yao xue xue bao = Acta pharmaceutica Sinica

SN - 0513-4870

IS - 11

ER -