Effects of granulocyte-macrophage colony-stimulating factor and tumor necrosis factor alpha on Trypanosoma cruzi trypomastigotes

Elizabeth Olivares Fontt, Patrick De Baetselier, Carlo Heirman, Kris Thielemans, Rudolf Lucas, Bernard Vray

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have previously shown that the addition of exogenous granulocyte- macrophage colony-stimulating factor (GM-CSF) to nonactivated mouse peritoneal macrophages (MPM) limits Trypanosoma cruzi infections in vitro (E. Olivares Fontt and B. Vray, Parasite Immunol. 17:135-141, 1995). Lower levels of infection were correlated with a higher level of production of tumor necrosis factor alpha (TNF-α) in the absence of nitric oxide (NO) release. These data suggested that GM-CSF and/or TNF-α might have a direct parasitocidal effect on T. cruzi trypomastigotes, independently of NO release. To address this question, T. cruzi trypomastigotes were treated with recombinant murine GM-CSF (rmGM-CSF), recombinant murine TNF-α (rmTNF-α), or both cytokines in a cell-free system. Treatment with rmGM-CSF but not rmTNF-α caused morphological changes in the parasites, and most became spherical after 7 b of incubation. Both cytokines exerted a cytolytic activity on the trypomastigotes, yet the trypanolytic activity of rmTNF-α was more effective than that of rmGM-CSF. Viable rmGM-CSF- and rmTNF-α- treated parasites were less able to infect MPM than untreated parasites, and this reduction in infectivity was greatest for rmGM-CSF. Treatments with both cytokines resulted in more lysis and almost complete inhibition of infection. The direct parasitocidal activity of rmTNF-α was inhibited by carbohydrates and monoclonal antibodies specific for the lectin-like domain of TNF-α. Collectively, these results suggest that cytokines such as GM-CSF and TNF-α may directly control the level of T. cruzi trypomastigotes at least in vitro and so could determine the outcome of infection in vivo.

Original languageEnglish (US)
Pages (from-to)2722-2727
Number of pages6
JournalInfection and Immunity
Volume66
Issue number6
StatePublished - Jun 1 1998
Externally publishedYes

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Trypanosoma cruzi
Granulocyte-Macrophage Colony-Stimulating Factor
Tumor Necrosis Factor-alpha
Parasites
Cytokines
Peritoneal Macrophages
Infection
Nitric Oxide
Cell-Free System
Lectins
Monoclonal Antibodies
Carbohydrates

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Effects of granulocyte-macrophage colony-stimulating factor and tumor necrosis factor alpha on Trypanosoma cruzi trypomastigotes. / Fontt, Elizabeth Olivares; De Baetselier, Patrick; Heirman, Carlo; Thielemans, Kris; Lucas, Rudolf; Vray, Bernard.

In: Infection and Immunity, Vol. 66, No. 6, 01.06.1998, p. 2722-2727.

Research output: Contribution to journalArticle

Fontt, EO, De Baetselier, P, Heirman, C, Thielemans, K, Lucas, R & Vray, B 1998, 'Effects of granulocyte-macrophage colony-stimulating factor and tumor necrosis factor alpha on Trypanosoma cruzi trypomastigotes', Infection and Immunity, vol. 66, no. 6, pp. 2722-2727.
Fontt, Elizabeth Olivares ; De Baetselier, Patrick ; Heirman, Carlo ; Thielemans, Kris ; Lucas, Rudolf ; Vray, Bernard. / Effects of granulocyte-macrophage colony-stimulating factor and tumor necrosis factor alpha on Trypanosoma cruzi trypomastigotes. In: Infection and Immunity. 1998 ; Vol. 66, No. 6. pp. 2722-2727.
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