Effects of oral consumption of the green tea polyphenol EGCG in a murine model for human Sjogren's syndrome, an autoimmune disease

Kevin Gillespie, Isamu Kodani, Douglas P. Dickinson, Kalu U.E. Ogbureke, Amy M. Camba, Mengjie Wu, Stephen Warwick Looney, Tin Chun Chu, Haiyan Qin, Frederick Bisch, Mohamed M.H. Sharawy, George S. Schuster, Stephen Hsu

Research output: Contribution to journalArticle

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Abstract

Significance: Protection of glandular cells from autoimmune-induced damage would be of significant clinical benefit to Sjogren's syndrome (SS) patients. Epigallocatechin-3-gallate (EGCG) possesses anti-apoptotic, anti-inflammatory, and autoantigen-inhibitory properties. Aims: To investigate if EGCG protects against certain autoimmune-induced pathological changes in the salivary glands of the non-obese diabetic (NOD) mouse model for SS. Main methods: Animals were provided with either water or water containing 0.2% EGCG. At the age of 8, 16 and 22 weeks, submandibular salivary gland tissue and serum samples were collected for pathological and serological analysis. Key findings: Significant lymphocyte infiltration was observed in the salivary glands of the water-fed group at the age of 16 weeks, while the EGCG group showed reduced lymphocyte infiltration. By 22 weeks of age, water-fed animals demonstrated elevated levels of apoptotic activity within the lymphocytic infiltrates, and high levels of serum total anti-nuclear antibody, compared to EGCG-fed animals. Remarkably, proliferating cell nuclear antigen (PCNA) and Ki-67 levels in the salivary glands of water-fed NOD mice were significantly elevated in comparison to BALB/c control mice; in contrast, PCNA and Ki-67 levels in EGCG-fed NOD animals were similar to BALB/c mice. These results indicate that EGCG protects the NOD mouse submandibular glands from autoimmune-induced inflammation, and reduces serum autoantibody levels. Abnormal proliferation, rather than apoptosis, appears to be a characteristic of the NOD mouse gland that is normalized by EGCG. The evidence suggests that EGCG could be useful in delaying or managing SS-like autoimmune disorders.

Original languageEnglish (US)
Pages (from-to)581-588
Number of pages8
JournalLife sciences
Volume83
Issue number17-18
DOIs
StatePublished - Oct 24 2008

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Sjogren's Syndrome
Polyphenols
Tea
Autoimmune Diseases
Inbred NOD Mouse
Salivary Glands
Animals
Water
Lymphocytes
Submandibular Gland
Proliferating Cell Nuclear Antigen
Infiltration
Serum
epigallocatechin gallate
Cytoprotection
Autoantigens
Autoantibodies
Anti-Idiotypic Antibodies
Anti-Inflammatory Agents
Age Groups

Keywords

  • Autoimmune disease
  • EGCG
  • Green tea
  • Non-obese diabetic mice
  • Sjogren's syndrome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Effects of oral consumption of the green tea polyphenol EGCG in a murine model for human Sjogren's syndrome, an autoimmune disease. / Gillespie, Kevin; Kodani, Isamu; Dickinson, Douglas P.; Ogbureke, Kalu U.E.; Camba, Amy M.; Wu, Mengjie; Looney, Stephen Warwick; Chu, Tin Chun; Qin, Haiyan; Bisch, Frederick; Sharawy, Mohamed M.H.; Schuster, George S.; Hsu, Stephen.

In: Life sciences, Vol. 83, No. 17-18, 24.10.2008, p. 581-588.

Research output: Contribution to journalArticle

Gillespie, K, Kodani, I, Dickinson, DP, Ogbureke, KUE, Camba, AM, Wu, M, Looney, SW, Chu, TC, Qin, H, Bisch, F, Sharawy, MMH, Schuster, GS & Hsu, S 2008, 'Effects of oral consumption of the green tea polyphenol EGCG in a murine model for human Sjogren's syndrome, an autoimmune disease', Life sciences, vol. 83, no. 17-18, pp. 581-588. https://doi.org/10.1016/j.lfs.2008.08.011
Gillespie, Kevin ; Kodani, Isamu ; Dickinson, Douglas P. ; Ogbureke, Kalu U.E. ; Camba, Amy M. ; Wu, Mengjie ; Looney, Stephen Warwick ; Chu, Tin Chun ; Qin, Haiyan ; Bisch, Frederick ; Sharawy, Mohamed M.H. ; Schuster, George S. ; Hsu, Stephen. / Effects of oral consumption of the green tea polyphenol EGCG in a murine model for human Sjogren's syndrome, an autoimmune disease. In: Life sciences. 2008 ; Vol. 83, No. 17-18. pp. 581-588.
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abstract = "Significance: Protection of glandular cells from autoimmune-induced damage would be of significant clinical benefit to Sjogren's syndrome (SS) patients. Epigallocatechin-3-gallate (EGCG) possesses anti-apoptotic, anti-inflammatory, and autoantigen-inhibitory properties. Aims: To investigate if EGCG protects against certain autoimmune-induced pathological changes in the salivary glands of the non-obese diabetic (NOD) mouse model for SS. Main methods: Animals were provided with either water or water containing 0.2{\%} EGCG. At the age of 8, 16 and 22 weeks, submandibular salivary gland tissue and serum samples were collected for pathological and serological analysis. Key findings: Significant lymphocyte infiltration was observed in the salivary glands of the water-fed group at the age of 16 weeks, while the EGCG group showed reduced lymphocyte infiltration. By 22 weeks of age, water-fed animals demonstrated elevated levels of apoptotic activity within the lymphocytic infiltrates, and high levels of serum total anti-nuclear antibody, compared to EGCG-fed animals. Remarkably, proliferating cell nuclear antigen (PCNA) and Ki-67 levels in the salivary glands of water-fed NOD mice were significantly elevated in comparison to BALB/c control mice; in contrast, PCNA and Ki-67 levels in EGCG-fed NOD animals were similar to BALB/c mice. These results indicate that EGCG protects the NOD mouse submandibular glands from autoimmune-induced inflammation, and reduces serum autoantibody levels. Abnormal proliferation, rather than apoptosis, appears to be a characteristic of the NOD mouse gland that is normalized by EGCG. The evidence suggests that EGCG could be useful in delaying or managing SS-like autoimmune disorders.",
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AU - Gillespie, Kevin

AU - Kodani, Isamu

AU - Dickinson, Douglas P.

AU - Ogbureke, Kalu U.E.

AU - Camba, Amy M.

AU - Wu, Mengjie

AU - Looney, Stephen Warwick

AU - Chu, Tin Chun

AU - Qin, Haiyan

AU - Bisch, Frederick

AU - Sharawy, Mohamed M.H.

AU - Schuster, George S.

AU - Hsu, Stephen

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AB - Significance: Protection of glandular cells from autoimmune-induced damage would be of significant clinical benefit to Sjogren's syndrome (SS) patients. Epigallocatechin-3-gallate (EGCG) possesses anti-apoptotic, anti-inflammatory, and autoantigen-inhibitory properties. Aims: To investigate if EGCG protects against certain autoimmune-induced pathological changes in the salivary glands of the non-obese diabetic (NOD) mouse model for SS. Main methods: Animals were provided with either water or water containing 0.2% EGCG. At the age of 8, 16 and 22 weeks, submandibular salivary gland tissue and serum samples were collected for pathological and serological analysis. Key findings: Significant lymphocyte infiltration was observed in the salivary glands of the water-fed group at the age of 16 weeks, while the EGCG group showed reduced lymphocyte infiltration. By 22 weeks of age, water-fed animals demonstrated elevated levels of apoptotic activity within the lymphocytic infiltrates, and high levels of serum total anti-nuclear antibody, compared to EGCG-fed animals. Remarkably, proliferating cell nuclear antigen (PCNA) and Ki-67 levels in the salivary glands of water-fed NOD mice were significantly elevated in comparison to BALB/c control mice; in contrast, PCNA and Ki-67 levels in EGCG-fed NOD animals were similar to BALB/c mice. These results indicate that EGCG protects the NOD mouse submandibular glands from autoimmune-induced inflammation, and reduces serum autoantibody levels. Abnormal proliferation, rather than apoptosis, appears to be a characteristic of the NOD mouse gland that is normalized by EGCG. The evidence suggests that EGCG could be useful in delaying or managing SS-like autoimmune disorders.

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KW - Non-obese diabetic mice

KW - Sjogren's syndrome

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