Effects of oxidized regenerated cellulose on the expression of extracellular matrix and transforming growth factor-β1 in human peritoneal fibroblasts and mesothelial cells

L. April Gago, Ghassan M. Saed, Rona X. Wang, Michael Kruger, Michael Peter Diamond, Kimberly Kenton

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

OBJECTIVE: The purpose of this study was to evaluate the potential biologic effects of oxidized regenerated cellulose, which has been shown in multiple human in vivo studies to reduce postoperative adhesion development, on the messenger RNA levels of transforming growth factor-β1, type I collagen, type 111 collagen, and fibronectin. STUDY DESIGN: The oxidized regenerated cellulose was dissolved in saline solution and added to confluent, monolayer cultures of human normal fibroblasts and mesothelial cells. Control cells were maintained in media alone at the same pH. After 24 hours of treatment, total RNA was extracted from all cells. Real-time reverse transcription-polymerase chain reaction was performed to determine the relative change in messenger RNA levels of type I, type HI collagen, fibronectin, transforming growth factor-β1, and β-actin (housekeeping gene) in response to the oxidized regenerated cellulose treatment (n = 4 cultures). Student t tests were performed for each cell type, which compared oxidized regenerated cellulose-treated cells to control cells. Calculated power for the statistically significant findings ranged from 65% to 100%. RESULTS: Transforming growth factor-β1 messenger RNA was elevated by the oxidized regenerated cellulose treatment in the mesothelial cells by 13% (control cells, 0.562 ± 0.022; oxidized regenerated cellulose-treated cells, 0.636 ± 0.014; P = .03). In normal fibroblasts, transforming growth factor-β1 messenger RNA was slightly, but not significantly, decreased in oxidized regenerated cellulose-exposed normal fibroblasts compared with controls (control cells, 0.622 ± 0.062; oxidized regenerated cellulose-treated cells, 0.609 ± 0.006; P = .85). Type I collagen was found to be increased by exposure to oxidized regenerated cellulose in both mesothelial cells and normal peritoneal fibroblasts. Type I collagen was increased by 23% in mesothelial cells (control cells [0.587 ± 0.018] vs oxidized regenerated cellulose-treated cells [0.722 ± 0.010], P = .002), and 27% in normal fibroblasts (control cells, 0.522 ± 0.018, oxidized regenerated cellulose-treated cells, 0.665 ± 0.009; P = .001). However, messenger RNA levels of type III collagen and fibronectin (other representative molecules of the extracellular matrix) were not altered significantly by oxidized regenerated cellulose application in vitro. CONCLUSION: Oxidized regenerated cellulose influences the expression of factors that are accepted commonly to be associated with adhesiogenesis. Oxidized regenerated cellulose increased the expression of transforming growth factor-β1 in mesothelial cells and type I collagen in mesothelial cells and normal peritoneal fibroblasts. Minimization of these oxidized regenerated cellulose-induced molecular changes, if they occur in vivo, may improve the ability of oxidized regenerated cellulose to reduce postoperative adhesion development.

Original languageEnglish (US)
Pages (from-to)1620-1625
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume189
Issue number6
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

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oxidized cellulose
Transforming Growth Factors
Extracellular Matrix
Fibroblasts
Collagen Type I
Messenger RNA
Fibronectins

Keywords

  • Adhesion
  • Extracellular matrix
  • Fibroblast
  • Mesothelial cell
  • Oxidized regenerated cellulose
  • Transforming growth factor-β1

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Effects of oxidized regenerated cellulose on the expression of extracellular matrix and transforming growth factor-β1 in human peritoneal fibroblasts and mesothelial cells. / Gago, L. April; Saed, Ghassan M.; Wang, Rona X.; Kruger, Michael; Diamond, Michael Peter; Kenton, Kimberly.

In: American Journal of Obstetrics and Gynecology, Vol. 189, No. 6, 01.01.2003, p. 1620-1625.

Research output: Contribution to journalArticle

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T1 - Effects of oxidized regenerated cellulose on the expression of extracellular matrix and transforming growth factor-β1 in human peritoneal fibroblasts and mesothelial cells

AU - Gago, L. April

AU - Saed, Ghassan M.

AU - Wang, Rona X.

AU - Kruger, Michael

AU - Diamond, Michael Peter

AU - Kenton, Kimberly

PY - 2003/1/1

Y1 - 2003/1/1

N2 - OBJECTIVE: The purpose of this study was to evaluate the potential biologic effects of oxidized regenerated cellulose, which has been shown in multiple human in vivo studies to reduce postoperative adhesion development, on the messenger RNA levels of transforming growth factor-β1, type I collagen, type 111 collagen, and fibronectin. STUDY DESIGN: The oxidized regenerated cellulose was dissolved in saline solution and added to confluent, monolayer cultures of human normal fibroblasts and mesothelial cells. Control cells were maintained in media alone at the same pH. After 24 hours of treatment, total RNA was extracted from all cells. Real-time reverse transcription-polymerase chain reaction was performed to determine the relative change in messenger RNA levels of type I, type HI collagen, fibronectin, transforming growth factor-β1, and β-actin (housekeeping gene) in response to the oxidized regenerated cellulose treatment (n = 4 cultures). Student t tests were performed for each cell type, which compared oxidized regenerated cellulose-treated cells to control cells. Calculated power for the statistically significant findings ranged from 65% to 100%. RESULTS: Transforming growth factor-β1 messenger RNA was elevated by the oxidized regenerated cellulose treatment in the mesothelial cells by 13% (control cells, 0.562 ± 0.022; oxidized regenerated cellulose-treated cells, 0.636 ± 0.014; P = .03). In normal fibroblasts, transforming growth factor-β1 messenger RNA was slightly, but not significantly, decreased in oxidized regenerated cellulose-exposed normal fibroblasts compared with controls (control cells, 0.622 ± 0.062; oxidized regenerated cellulose-treated cells, 0.609 ± 0.006; P = .85). Type I collagen was found to be increased by exposure to oxidized regenerated cellulose in both mesothelial cells and normal peritoneal fibroblasts. Type I collagen was increased by 23% in mesothelial cells (control cells [0.587 ± 0.018] vs oxidized regenerated cellulose-treated cells [0.722 ± 0.010], P = .002), and 27% in normal fibroblasts (control cells, 0.522 ± 0.018, oxidized regenerated cellulose-treated cells, 0.665 ± 0.009; P = .001). However, messenger RNA levels of type III collagen and fibronectin (other representative molecules of the extracellular matrix) were not altered significantly by oxidized regenerated cellulose application in vitro. CONCLUSION: Oxidized regenerated cellulose influences the expression of factors that are accepted commonly to be associated with adhesiogenesis. Oxidized regenerated cellulose increased the expression of transforming growth factor-β1 in mesothelial cells and type I collagen in mesothelial cells and normal peritoneal fibroblasts. Minimization of these oxidized regenerated cellulose-induced molecular changes, if they occur in vivo, may improve the ability of oxidized regenerated cellulose to reduce postoperative adhesion development.

AB - OBJECTIVE: The purpose of this study was to evaluate the potential biologic effects of oxidized regenerated cellulose, which has been shown in multiple human in vivo studies to reduce postoperative adhesion development, on the messenger RNA levels of transforming growth factor-β1, type I collagen, type 111 collagen, and fibronectin. STUDY DESIGN: The oxidized regenerated cellulose was dissolved in saline solution and added to confluent, monolayer cultures of human normal fibroblasts and mesothelial cells. Control cells were maintained in media alone at the same pH. After 24 hours of treatment, total RNA was extracted from all cells. Real-time reverse transcription-polymerase chain reaction was performed to determine the relative change in messenger RNA levels of type I, type HI collagen, fibronectin, transforming growth factor-β1, and β-actin (housekeeping gene) in response to the oxidized regenerated cellulose treatment (n = 4 cultures). Student t tests were performed for each cell type, which compared oxidized regenerated cellulose-treated cells to control cells. Calculated power for the statistically significant findings ranged from 65% to 100%. RESULTS: Transforming growth factor-β1 messenger RNA was elevated by the oxidized regenerated cellulose treatment in the mesothelial cells by 13% (control cells, 0.562 ± 0.022; oxidized regenerated cellulose-treated cells, 0.636 ± 0.014; P = .03). In normal fibroblasts, transforming growth factor-β1 messenger RNA was slightly, but not significantly, decreased in oxidized regenerated cellulose-exposed normal fibroblasts compared with controls (control cells, 0.622 ± 0.062; oxidized regenerated cellulose-treated cells, 0.609 ± 0.006; P = .85). Type I collagen was found to be increased by exposure to oxidized regenerated cellulose in both mesothelial cells and normal peritoneal fibroblasts. Type I collagen was increased by 23% in mesothelial cells (control cells [0.587 ± 0.018] vs oxidized regenerated cellulose-treated cells [0.722 ± 0.010], P = .002), and 27% in normal fibroblasts (control cells, 0.522 ± 0.018, oxidized regenerated cellulose-treated cells, 0.665 ± 0.009; P = .001). However, messenger RNA levels of type III collagen and fibronectin (other representative molecules of the extracellular matrix) were not altered significantly by oxidized regenerated cellulose application in vitro. CONCLUSION: Oxidized regenerated cellulose influences the expression of factors that are accepted commonly to be associated with adhesiogenesis. Oxidized regenerated cellulose increased the expression of transforming growth factor-β1 in mesothelial cells and type I collagen in mesothelial cells and normal peritoneal fibroblasts. Minimization of these oxidized regenerated cellulose-induced molecular changes, if they occur in vivo, may improve the ability of oxidized regenerated cellulose to reduce postoperative adhesion development.

KW - Adhesion

KW - Extracellular matrix

KW - Fibroblast

KW - Mesothelial cell

KW - Oxidized regenerated cellulose

KW - Transforming growth factor-β1

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