Effects of pentoxifylline on the adherence of polymorphonuclear neutrophils to oxidant-stimulated human endothelial cells: Involvement of cyclic amp

Elisabeth Franzini, Hassan Sellak, Catherine Babin-Chevaye, Jacques Hakim, Catherine Pasquier

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16 Scopus citations

Abstract

Cultured human umbilical vein endothelial cells (HUVECs) treated with reactive oxygen species (ROS) show increased adherence of polymorphonuclear leukocytes (PMNs). Because pentoxifylline (PTX) is known to inhibit cell interactions, we studied PMN adherence to ROS-stimulated HUVECs pretreated with PTX. ROS were generated by the oxidation of hypoxanthine by xanthine oxidase, giving rise to superoxide anion and hydrogen peroxide. Human PMNs were then added to HUVEC monolayers. After various times, the cultures were washed and the number of adherent PMNs was estimated by measuring myeloperoxidase in the total cell homogenate. PTX inhibited adherence in a concentration-dependent manner. Moreover, the increase in intracellular cAMP content varied with the PTX concentration. Isobutylmethylxanthine (IBMX) and isoproterenol (ISO), which increase intracellular cAMP content, also inhibited the adherence of PMNs to ROS-stimulated HUVECs, We conclude that cAMP is probably involved in the intracellular regulation of ROS-mediated PMN adherence to endothelial cells.

Original languageEnglish (US)
Pages (from-to)S92-S95
JournalJournal of Cardiovascular Pharmacology
Volume25
DOIs
Publication statusPublished - Jan 1 1995

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Keywords

  • Adherence
  • Cyclic AMP
  • Hydrogen peroxide
  • Hypoxanthine
  • Pentoxifylline
  • Reactive oxygen species
  • Xanthine oxidase

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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