Effects of sub-toxic concentrations of camphorquinone on cell lipid metabolism

Rahul A. Datar, Frederick Rueggeberg, Gretchen B Caughman, John C. Wataha, Jill B. Lewis, George S. Schuster

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The biological effects of camphorquinone (CQ), an initiator for light-polymerized resins, have been reported to relate to its ability to generate free radicals and cause radical-induced membrane damage via lipid peroxidation. However, the effects of CQ on lipids other than peroxidation may result in unfavorable tissue responses especially at concentrations that are not overtly toxic to cells. The purpose of the current study was to examine the effects of CQ on cell lipid metabolism at subtoxic concentrations, with or without visible light irradiation. HCP and THP-1 cells were exposed to CQ with or without light irradiation under clinically relevant conditions and lipid metabolism was analyzed using 14C-labeling and thin-layer chromatography. We found that CQ increased synthesis of neutral lipids, such as triglycerides, from 7 to nearly 15% of the total and diglycerides from 2% to about 3% of the total in HCP cells, while synthesis of phospholipids, such as sphingomyelin, was decreased by 1-1.5%. In THP-1 cells cholesterol synthesis increased more than 2-fold and cholesterol ester synthesis increased more than 5-fold. Light-activated CQ did not differ significantly in terms of its bioactivity compared to no-light conditions. We conclude that CQ significantly altered the metabolism of several important structural lipids in two cell types at sub-toxic concentrations that are clinically relevant. These changes in lipid metabolism may in turn affect membrane integrity and permeability and possibly lead to significant changes in cell responses.

Original languageEnglish (US)
Pages (from-to)1293-1302
Number of pages10
JournalJournal of Biomaterials Science, Polymer Edition
Volume16
Issue number10
DOIs
StatePublished - Oct 1 2005

Fingerprint

Poisons
Lipid Metabolism
Lipids
Light
Cholesterol
Irradiation
Membranes
Lipid Peroxidation
Thin layer chromatography
Phospholipids
Bioactivity
Free radicals
Metabolism
Labeling
Sphingomyelins
Esters
Cholesterol Esters
Diglycerides
Resins
Thin Layer Chromatography

Keywords

  • Camphorquinone
  • Epithelial cells
  • Light irradiation
  • Lipid metabolism
  • Monocytes

ASJC Scopus subject areas

  • Bioengineering
  • Biophysics
  • Biomaterials
  • Biomedical Engineering

Cite this

Effects of sub-toxic concentrations of camphorquinone on cell lipid metabolism. / Datar, Rahul A.; Rueggeberg, Frederick; Caughman, Gretchen B; Wataha, John C.; Lewis, Jill B.; Schuster, George S.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 16, No. 10, 01.10.2005, p. 1293-1302.

Research output: Contribution to journalArticle

Datar, Rahul A. ; Rueggeberg, Frederick ; Caughman, Gretchen B ; Wataha, John C. ; Lewis, Jill B. ; Schuster, George S. / Effects of sub-toxic concentrations of camphorquinone on cell lipid metabolism. In: Journal of Biomaterials Science, Polymer Edition. 2005 ; Vol. 16, No. 10. pp. 1293-1302.
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