Effects of the antiestrogen CI-628 on hCG-induced desensitization of testosterone in purified leydig cells

B. A. Keel, M. H. Melner, T. O. Abney

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The effect of CI-628 on human chorionic gonadotropin-induced desensitization of testosterone production was investigated. Purified Leydig cells were isolated from mature male rats 24 hr postinjection with (1) 30 of 300 IU hCG s.c., (2) 1 mg CI-628 i.p., or (3) CI-628 pretreatment plus hCG. Steroidogenic desensitization to Bt2cAMP and hCG stimulation in vitro was observed with both doses of hCG administered in vivo. CI-628 treatment alone led to an increased basal production but an unaltered maximum production of testosterone obtained in the presence of hCG or Bt2cAMP in vitro. CI-628 in combination with hCG treatment in vivo led to testosterone production in vitro that was similar to that observed with hCG treatment alone. CI-628 does not inhibit the hCG-induced desensitization of testosterone production, and the results do not support a role of estrogen receptors in this process.

Original languageEnglish (US)
Pages (from-to)71-75
Number of pages5
JournalSystems Biology in Reproductive Medicine
Volume13
Issue number1
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

Keywords

  • Antiestrogen
  • Desensitization
  • Leydig cell
  • Testis
  • Testosterone

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

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