Abstract
The effect of CI-628 on human chorionic gonadotropin-induced desensitization of testosterone production was investigated. Purified Leydig cells were isolated from mature male rats 24 hr postinjection with (1) 30 of 300 IU hCG s.c., (2) 1 mg CI-628 i.p., or (3) CI-628 pretreatment plus hCG. Steroidogenic desensitization to Bt2cAMP and hCG stimulation in vitro was observed with both doses of hCG administered in vivo. CI-628 treatment alone led to an increased basal production but an unaltered maximum production of testosterone obtained in the presence of hCG or Bt2cAMP in vitro. CI-628 in combination with hCG treatment in vivo led to testosterone production in vitro that was similar to that observed with hCG treatment alone. CI-628 does not inhibit the hCG-induced desensitization of testosterone production, and the results do not support a role of estrogen receptors in this process.
Original language | English (US) |
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Pages (from-to) | 71-75 |
Number of pages | 5 |
Journal | Systems Biology in Reproductive Medicine |
Volume | 13 |
Issue number | 1 |
DOIs | |
State | Published - 1984 |
Externally published | Yes |
Keywords
- Antiestrogen
- Desensitization
- Leydig cell
- Testis
- Testosterone
ASJC Scopus subject areas
- Reproductive Medicine
- Urology