Effects of the antiestrogen CI-628 on hCG-induced desensitization of testosterone in purified leydig cells

Brooks Allen Keel, M. H. Melner, T. O. Abney

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effect of CI-628 on human chorionic gonadotropin-induced desensitization of testosterone production was investigated. Purified Leydig cells were isolated from mature male rats 24 hr postinjection with (1) 30 of 300 IU hCG s.c., (2) 1 mg CI-628 i.p., or (3) CI-628 pretreatment plus hCG. Steroidogenic desensitization to Bt2cAMP and hCG stimulation in vitro was observed with both doses of hCG administered in vivo. CI-628 treatment alone led to an increased basal production but an unaltered maximum production of testosterone obtained in the presence of hCG or Bt2cAMP in vitro. CI-628 in combination with hCG treatment in vivo led to testosterone production in vitro that was similar to that observed with hCG treatment alone. CI-628 does not inhibit the hCG-induced desensitization of testosterone production, and the results do not support a role of estrogen receptors in this process.

Original languageEnglish (US)
Pages (from-to)71-75
Number of pages5
JournalSystems Biology in Reproductive Medicine
Volume13
Issue number1
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

Nitromifene
Estrogen Receptor Modulators
Leydig Cells
Testosterone
Chorionic Gonadotropin
Estrogen Receptors

Keywords

  • Antiestrogen
  • Desensitization
  • Leydig cell
  • Testis
  • Testosterone

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

Cite this

Effects of the antiestrogen CI-628 on hCG-induced desensitization of testosterone in purified leydig cells. / Keel, Brooks Allen; Melner, M. H.; Abney, T. O.

In: Systems Biology in Reproductive Medicine, Vol. 13, No. 1, 01.01.1984, p. 71-75.

Research output: Contribution to journalArticle

@article{b628a38b4d21442ab71357ab0426ea81,
title = "Effects of the antiestrogen CI-628 on hCG-induced desensitization of testosterone in purified leydig cells",
abstract = "The effect of CI-628 on human chorionic gonadotropin-induced desensitization of testosterone production was investigated. Purified Leydig cells were isolated from mature male rats 24 hr postinjection with (1) 30 of 300 IU hCG s.c., (2) 1 mg CI-628 i.p., or (3) CI-628 pretreatment plus hCG. Steroidogenic desensitization to Bt2cAMP and hCG stimulation in vitro was observed with both doses of hCG administered in vivo. CI-628 treatment alone led to an increased basal production but an unaltered maximum production of testosterone obtained in the presence of hCG or Bt2cAMP in vitro. CI-628 in combination with hCG treatment in vivo led to testosterone production in vitro that was similar to that observed with hCG treatment alone. CI-628 does not inhibit the hCG-induced desensitization of testosterone production, and the results do not support a role of estrogen receptors in this process.",
keywords = "Antiestrogen, Desensitization, Leydig cell, Testis, Testosterone",
author = "Keel, {Brooks Allen} and Melner, {M. H.} and Abney, {T. O.}",
year = "1984",
month = "1",
day = "1",
doi = "10.3109/01485018408987502",
language = "English (US)",
volume = "13",
pages = "71--75",
journal = "Systems Biology in Reproductive Medicine",
issn = "1939-6368",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Effects of the antiestrogen CI-628 on hCG-induced desensitization of testosterone in purified leydig cells

AU - Keel, Brooks Allen

AU - Melner, M. H.

AU - Abney, T. O.

PY - 1984/1/1

Y1 - 1984/1/1

N2 - The effect of CI-628 on human chorionic gonadotropin-induced desensitization of testosterone production was investigated. Purified Leydig cells were isolated from mature male rats 24 hr postinjection with (1) 30 of 300 IU hCG s.c., (2) 1 mg CI-628 i.p., or (3) CI-628 pretreatment plus hCG. Steroidogenic desensitization to Bt2cAMP and hCG stimulation in vitro was observed with both doses of hCG administered in vivo. CI-628 treatment alone led to an increased basal production but an unaltered maximum production of testosterone obtained in the presence of hCG or Bt2cAMP in vitro. CI-628 in combination with hCG treatment in vivo led to testosterone production in vitro that was similar to that observed with hCG treatment alone. CI-628 does not inhibit the hCG-induced desensitization of testosterone production, and the results do not support a role of estrogen receptors in this process.

AB - The effect of CI-628 on human chorionic gonadotropin-induced desensitization of testosterone production was investigated. Purified Leydig cells were isolated from mature male rats 24 hr postinjection with (1) 30 of 300 IU hCG s.c., (2) 1 mg CI-628 i.p., or (3) CI-628 pretreatment plus hCG. Steroidogenic desensitization to Bt2cAMP and hCG stimulation in vitro was observed with both doses of hCG administered in vivo. CI-628 treatment alone led to an increased basal production but an unaltered maximum production of testosterone obtained in the presence of hCG or Bt2cAMP in vitro. CI-628 in combination with hCG treatment in vivo led to testosterone production in vitro that was similar to that observed with hCG treatment alone. CI-628 does not inhibit the hCG-induced desensitization of testosterone production, and the results do not support a role of estrogen receptors in this process.

KW - Antiestrogen

KW - Desensitization

KW - Leydig cell

KW - Testis

KW - Testosterone

UR - http://www.scopus.com/inward/record.url?scp=84907133526&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907133526&partnerID=8YFLogxK

U2 - 10.3109/01485018408987502

DO - 10.3109/01485018408987502

M3 - Article

C2 - 6100037

AN - SCOPUS:84907133526

VL - 13

SP - 71

EP - 75

JO - Systems Biology in Reproductive Medicine

JF - Systems Biology in Reproductive Medicine

SN - 1939-6368

IS - 1

ER -