Effects of water and water-free polar solvents on the tensile properties of demineralized dentin

David Henry Pashley, Kelli A. Agee, Ricardo M. Carvalho, Kwang Won Lee, Franklin Chi Meng Tay, Terry E. Callison

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Objectives. The aim of this study was to test the null hypothesis that the tensile properties of demineralized dentin are not influenced by the hydrogen bonding ability of anhydrous polar solvents. Methods. Dentin disks 0.5mm thick were prepared from mid-coronal dentin of extracted, unerupted, human third molars. 'I' beam and hour-glass shaped specimens were prepared from the disks, the ends protected with nail varnish and the central regions completely demineralized in 0.5 M EDTA for 5 days. Ultimate tensile stress (UTS) and low-strain apparent modulus of elasticity (E) were determined with the specimens immersed for 60min in water, methanol, HEMA, acetone or air prior to testing in those same media. Apparent moduli of elasticity were measured on the same specimens in a repeated measures experimental design. The results were analyzed with a one-way ANOVA on ranks, followed by Dunn's test at α = 0.05. Regression analysis examined the relationship between UTS or E and Hansen's solubility parameter for hydrogen bonding (δh) of each solvent. Results. The UTS of demineralized dentin in water, methanol, HEMA, acetone and air was 18(7), 29(7), 31(6), 41(13) and 146(27)MPa, x̄(SD), n = 10. Low-strain E for the same media were 11(7), 43(12), 79(21), 132(31) and 253(115)MPa. Regression analysis of UTS vs δh revealed a significant (p < 0.0005, r = -0.69, R2 = 0.48) inverse, exponential relationship. A similar inverse relationship was obtained between low-strain E vs δh (p < 0.0001, r = -0.93, R2 = 0.86). Significance. The tensile properties of demineralized dentin are dependent on the hydrogen bonding ability of polar solvents (δh). Solvents with low δh values may permit new interpeptide H-bonding in collagen that increases its tensile properties. Solvents with high δh values prevent the development of these new interpeptide H-bonds.

Original languageEnglish (US)
Pages (from-to)347-352
Number of pages6
JournalDental Materials
Volume19
Issue number5
DOIs
StatePublished - Jul 1 2003

Fingerprint

Dentin
Tensile properties
Tensile stress
Hydrogen Bonding
Water
Hydrogen bonds
Elastic Modulus
Acetone
Regression analysis
Methanol
Elastic moduli
Air
Regression Analysis
Varnish
Nails
Third Molar
Paint
Ethylenediaminetetraacetic acid
Analysis of variance (ANOVA)
Collagen

Keywords

  • Collagen
  • Hydrogen bonding
  • Modulus of elasticity
  • Solubility parameters
  • Tensile properties
  • Ultimate tensile stress

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Effects of water and water-free polar solvents on the tensile properties of demineralized dentin. / Pashley, David Henry; Agee, Kelli A.; Carvalho, Ricardo M.; Lee, Kwang Won; Tay, Franklin Chi Meng; Callison, Terry E.

In: Dental Materials, Vol. 19, No. 5, 01.07.2003, p. 347-352.

Research output: Contribution to journalArticle

Pashley, David Henry ; Agee, Kelli A. ; Carvalho, Ricardo M. ; Lee, Kwang Won ; Tay, Franklin Chi Meng ; Callison, Terry E. / Effects of water and water-free polar solvents on the tensile properties of demineralized dentin. In: Dental Materials. 2003 ; Vol. 19, No. 5. pp. 347-352.
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