Efficacy and biocompatibility of a photopolymerized, synthetic, absorbable hydrogel as a dural sealant in a canine craniotomy model

Cargill H. Alleyne, C. Michael Cawley, Daniel L. Barrow, Bradley C. Poff, Michelle D. Powell, Amarpreet S. Sawhney, Dirck L. Dillehay

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Object. A canine craniotomy model was used to evaluate the dural sealing efficacy and biocompatibility of a novel, synthetic, bioresorbable hydrogel. Methods. Bilateral craniotomies were performed in 24 dogs assigned to six survival periods. In each animal a parasagittal durotomy was created and then repaired. At the treatment sites the hydrogel sealant was applied over the dural repair and photopolymerized. The repair was tested for leaks to 20 cm H2O by using a Valsalva maneuver. At the control sites the incisions were sutured and tested for leaks only. After uneventful survival periods, the leak test was repeated in three of the four animals in each group. Bone-dura adhesion was evaluated, after which the dura and underlying brain were removed, fixed, and examined histologically. En bloc histological investigation was performed on a specimen obtained from the fourth animal in each group. Over a 56-day period, 18 treated sites were tested for leaks. A leak was detected at a site remote from that of the repair in one animal; this was excluded from analysis. Thus 17 of 17 treated sites remained free of leaks. On the control side of one animal, there was a leak from a new dural tear at the cranial end of the durotomy, which occurred when the bone flap was removed. This site was also excluded from analysis. Eleven of 17 leak- tested control sites remained free of leaks over the study period. Bone-dura adhesions occurred in 15 of 19 control sites and had a mean adhesion score of 1.37 (range 0-4), whereas adhesions occurred in 10 of 19 treated sites with a mean adhesion score of 0.84 (range 0-3). No cortical reaction was noted. Conclusions. This novel hydrogel sealant is efficacious in sealing rural repair sites measuring up to 2 mm. Healing of the underlying dura is not compromised and exposed cortical tissue is not altered histologically.

Original languageEnglish (US)
Pages (from-to)308-313
Number of pages6
JournalJournal of neurosurgery
Volume88
Issue number2
DOIs
StatePublished - Feb 1998

Fingerprint

Craniotomy
Hydrogel
Canidae
Bone and Bones
Valsalva Maneuver
Tears
Dogs
Brain

Keywords

  • Cerebrospinal fluid leak
  • Dog
  • Dural defect repair
  • Dural sealant
  • Dural tear
  • Hydrogel
  • Meninges

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

Cite this

Alleyne, C. H., Cawley, C. M., Barrow, D. L., Poff, B. C., Powell, M. D., Sawhney, A. S., & Dillehay, D. L. (1998). Efficacy and biocompatibility of a photopolymerized, synthetic, absorbable hydrogel as a dural sealant in a canine craniotomy model. Journal of neurosurgery, 88(2), 308-313. https://doi.org/10.3171/jns.1998.88.2.0308

Efficacy and biocompatibility of a photopolymerized, synthetic, absorbable hydrogel as a dural sealant in a canine craniotomy model. / Alleyne, Cargill H.; Cawley, C. Michael; Barrow, Daniel L.; Poff, Bradley C.; Powell, Michelle D.; Sawhney, Amarpreet S.; Dillehay, Dirck L.

In: Journal of neurosurgery, Vol. 88, No. 2, 02.1998, p. 308-313.

Research output: Contribution to journalArticle

Alleyne, Cargill H. ; Cawley, C. Michael ; Barrow, Daniel L. ; Poff, Bradley C. ; Powell, Michelle D. ; Sawhney, Amarpreet S. ; Dillehay, Dirck L. / Efficacy and biocompatibility of a photopolymerized, synthetic, absorbable hydrogel as a dural sealant in a canine craniotomy model. In: Journal of neurosurgery. 1998 ; Vol. 88, No. 2. pp. 308-313.
@article{ef68d1ae1d4b4885a6ad7a807afa4912,
title = "Efficacy and biocompatibility of a photopolymerized, synthetic, absorbable hydrogel as a dural sealant in a canine craniotomy model",
abstract = "Object. A canine craniotomy model was used to evaluate the dural sealing efficacy and biocompatibility of a novel, synthetic, bioresorbable hydrogel. Methods. Bilateral craniotomies were performed in 24 dogs assigned to six survival periods. In each animal a parasagittal durotomy was created and then repaired. At the treatment sites the hydrogel sealant was applied over the dural repair and photopolymerized. The repair was tested for leaks to 20 cm H2O by using a Valsalva maneuver. At the control sites the incisions were sutured and tested for leaks only. After uneventful survival periods, the leak test was repeated in three of the four animals in each group. Bone-dura adhesion was evaluated, after which the dura and underlying brain were removed, fixed, and examined histologically. En bloc histological investigation was performed on a specimen obtained from the fourth animal in each group. Over a 56-day period, 18 treated sites were tested for leaks. A leak was detected at a site remote from that of the repair in one animal; this was excluded from analysis. Thus 17 of 17 treated sites remained free of leaks. On the control side of one animal, there was a leak from a new dural tear at the cranial end of the durotomy, which occurred when the bone flap was removed. This site was also excluded from analysis. Eleven of 17 leak- tested control sites remained free of leaks over the study period. Bone-dura adhesions occurred in 15 of 19 control sites and had a mean adhesion score of 1.37 (range 0-4), whereas adhesions occurred in 10 of 19 treated sites with a mean adhesion score of 0.84 (range 0-3). No cortical reaction was noted. Conclusions. This novel hydrogel sealant is efficacious in sealing rural repair sites measuring up to 2 mm. Healing of the underlying dura is not compromised and exposed cortical tissue is not altered histologically.",
keywords = "Cerebrospinal fluid leak, Dog, Dural defect repair, Dural sealant, Dural tear, Hydrogel, Meninges",
author = "Alleyne, {Cargill H.} and Cawley, {C. Michael} and Barrow, {Daniel L.} and Poff, {Bradley C.} and Powell, {Michelle D.} and Sawhney, {Amarpreet S.} and Dillehay, {Dirck L.}",
year = "1998",
month = "2",
doi = "10.3171/jns.1998.88.2.0308",
language = "English (US)",
volume = "88",
pages = "308--313",
journal = "Journal of Neurosurgery",
issn = "0022-3085",
publisher = "American Association of Neurological Surgeons",
number = "2",

}

TY - JOUR

T1 - Efficacy and biocompatibility of a photopolymerized, synthetic, absorbable hydrogel as a dural sealant in a canine craniotomy model

AU - Alleyne, Cargill H.

AU - Cawley, C. Michael

AU - Barrow, Daniel L.

AU - Poff, Bradley C.

AU - Powell, Michelle D.

AU - Sawhney, Amarpreet S.

AU - Dillehay, Dirck L.

PY - 1998/2

Y1 - 1998/2

N2 - Object. A canine craniotomy model was used to evaluate the dural sealing efficacy and biocompatibility of a novel, synthetic, bioresorbable hydrogel. Methods. Bilateral craniotomies were performed in 24 dogs assigned to six survival periods. In each animal a parasagittal durotomy was created and then repaired. At the treatment sites the hydrogel sealant was applied over the dural repair and photopolymerized. The repair was tested for leaks to 20 cm H2O by using a Valsalva maneuver. At the control sites the incisions were sutured and tested for leaks only. After uneventful survival periods, the leak test was repeated in three of the four animals in each group. Bone-dura adhesion was evaluated, after which the dura and underlying brain were removed, fixed, and examined histologically. En bloc histological investigation was performed on a specimen obtained from the fourth animal in each group. Over a 56-day period, 18 treated sites were tested for leaks. A leak was detected at a site remote from that of the repair in one animal; this was excluded from analysis. Thus 17 of 17 treated sites remained free of leaks. On the control side of one animal, there was a leak from a new dural tear at the cranial end of the durotomy, which occurred when the bone flap was removed. This site was also excluded from analysis. Eleven of 17 leak- tested control sites remained free of leaks over the study period. Bone-dura adhesions occurred in 15 of 19 control sites and had a mean adhesion score of 1.37 (range 0-4), whereas adhesions occurred in 10 of 19 treated sites with a mean adhesion score of 0.84 (range 0-3). No cortical reaction was noted. Conclusions. This novel hydrogel sealant is efficacious in sealing rural repair sites measuring up to 2 mm. Healing of the underlying dura is not compromised and exposed cortical tissue is not altered histologically.

AB - Object. A canine craniotomy model was used to evaluate the dural sealing efficacy and biocompatibility of a novel, synthetic, bioresorbable hydrogel. Methods. Bilateral craniotomies were performed in 24 dogs assigned to six survival periods. In each animal a parasagittal durotomy was created and then repaired. At the treatment sites the hydrogel sealant was applied over the dural repair and photopolymerized. The repair was tested for leaks to 20 cm H2O by using a Valsalva maneuver. At the control sites the incisions were sutured and tested for leaks only. After uneventful survival periods, the leak test was repeated in three of the four animals in each group. Bone-dura adhesion was evaluated, after which the dura and underlying brain were removed, fixed, and examined histologically. En bloc histological investigation was performed on a specimen obtained from the fourth animal in each group. Over a 56-day period, 18 treated sites were tested for leaks. A leak was detected at a site remote from that of the repair in one animal; this was excluded from analysis. Thus 17 of 17 treated sites remained free of leaks. On the control side of one animal, there was a leak from a new dural tear at the cranial end of the durotomy, which occurred when the bone flap was removed. This site was also excluded from analysis. Eleven of 17 leak- tested control sites remained free of leaks over the study period. Bone-dura adhesions occurred in 15 of 19 control sites and had a mean adhesion score of 1.37 (range 0-4), whereas adhesions occurred in 10 of 19 treated sites with a mean adhesion score of 0.84 (range 0-3). No cortical reaction was noted. Conclusions. This novel hydrogel sealant is efficacious in sealing rural repair sites measuring up to 2 mm. Healing of the underlying dura is not compromised and exposed cortical tissue is not altered histologically.

KW - Cerebrospinal fluid leak

KW - Dog

KW - Dural defect repair

KW - Dural sealant

KW - Dural tear

KW - Hydrogel

KW - Meninges

UR - http://www.scopus.com/inward/record.url?scp=2642609465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2642609465&partnerID=8YFLogxK

U2 - 10.3171/jns.1998.88.2.0308

DO - 10.3171/jns.1998.88.2.0308

M3 - Article

C2 - 9452241

AN - SCOPUS:2642609465

VL - 88

SP - 308

EP - 313

JO - Journal of Neurosurgery

JF - Journal of Neurosurgery

SN - 0022-3085

IS - 2

ER -