Efficiency of combined blocking of aerobic and glycolytic metabolism pathways in treatment of N1-S1 hepatocellular carcinoma in a rat model

Hooman Yarmohammadi, Luke Wilkins, Joseph Erinjeri, Ronald Novak, Agata Exner, Hanping Wu, Elena Petre, Edward Boas, Etay Ziv, John Haaga

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Background/Aim: The aim of this study was to determine whether the addition of bumetanide (BU), a glycolytic metabolism pathway inhibitor, to arterial embolization improves tumor necrosis of N1-S1 hepatocellular carcinoma in a rat model. Materials and Methods: N1-S1 tumors were surgically implanted in the liver of 14 Sprague-Dawley rats. The rats were divided into three groups: In control group (n = 5), 1 ml of normal saline was injected intra-arterially. The tumor in the transarterial embolization group (TAE, n = 4) was embolized using 10 mg of 50-150 μ polyvinyl alcohol (PVA) particles and embolization plus BU group (TAE + BU, n = 5) were embolized with 10 mg of PVA plus 0.04 mg/kg of BU. Tumor volume was measured using two-dimensional ultrasound before intervention and twice a week afterward. Relative tumor volume after the intervention was calculated as the percentage of preinterventional tumor volume. After 4 weeks of observation, the rats were sacrificed for histopathological evaluation. Results: No statistically significant difference was detected in the preintervention tumor sizes between the three groups (P > 0.05). In the control group, the relative tumor volume increased to 142.5% larger than baseline measurements. In the TAE group, the tumor volume decreased by 18.2 ± 12.2%. The tumor volume in the TAE + BU group decrease by 90.4 ± 10.2%, which was 72.2% more than in TAE only group (P < 0.0001). Histopathological evaluation demonstrated no residual tumor in the TAE + BU group. Conclusion: Tumor necrosis significantly increased in N1-S1 tumor that received BU at the time of TAE when compared to TAE alone.

Original languageEnglish (US)
Pages (from-to)533-537
Number of pages5
JournalJournal of Cancer Research and Therapeutics
Volume13
Issue number3
DOIs
StatePublished - Jul 1 2017
Externally publishedYes

Keywords

  • Aerobic metabolism
  • bumetanide
  • glycolytic inhibition
  • hepatocellular carcinoma
  • N1-S1
  • transarterial embolization

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging

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