Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique

Qiang Sun, Juan Dong, Wenting Yang, Yujuan Jin, Mingying Yang, Yan Wang, Philip L. Wang, Yinghe Hu, Joe Z. Tsien

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

One of the technical bottlenecks in producing nonhuman primate models is that current assisted reproductive techniques, such as in vitro culture and frozen conservation of multicell-stage embryos, often result in poor embryo quality and subsequently lead to low birth rates. We investigated whether pronuclear embryo transfer can be used as an effective means for improving pregnancy and live birth rates of nonhuman primates. We collected 174 metaphase II oocytes by laparoscopy from 22 superovulated mature females and then fertilized these eggs using either in vitro fertilization or intracytoplasmic sperm injection, resulting in a 33.3% and a 50% fertilization rate, respectively. These 66 fertilized pronuclear-stage embryos were then tubally transferred to 30 recipients and led to 7 births and 1 abortion. Importantly, we observed that the highest live birth rate of ≈64% was obtained when the transfer of pronuclear embryos was performed in the presence of new corpus luteum in the ovary of recipients between 24 h and 36 h after estradiol peak. Therefore, our experiments demonstrate that by matching the critical time window in the recipient's reproductive cycle for achieving optimal embryo-uterine synchrony, pronuclear embryo transfer technology can significantly improve the pregnancy rate and live birth of healthy baby monkeys. This efficient method should be valuable to the systematic efforts in construction of various transgenic primate disease models.

Original languageEnglish (US)
Pages (from-to)12956-12960
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number35
DOIs
StatePublished - Sep 2 2008

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Macaca fascicularis
Embryo Transfer
Reproduction
Embryonic Structures
Birth Rate
Pregnancy Rate
Primates
Primate Diseases
Assisted Reproductive Techniques
Intracytoplasmic Sperm Injections
Zygote
Corpus Luteum
Live Birth
Fertilization in Vitro
Metaphase
Fertilization
Laparoscopy
Oocytes
Haplorhini
Ovary

Keywords

  • Embryo-uterine synchrony
  • Intracytoplasmic sperm injection
  • Test-tube monkey
  • Transgenic monkey
  • in vitro fertilization

ASJC Scopus subject areas

  • General

Cite this

Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique. / Sun, Qiang; Dong, Juan; Yang, Wenting; Jin, Yujuan; Yang, Mingying; Wang, Yan; Wang, Philip L.; Hu, Yinghe; Tsien, Joe Z.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 35, 02.09.2008, p. 12956-12960.

Research output: Contribution to journalArticle

Sun, Qiang ; Dong, Juan ; Yang, Wenting ; Jin, Yujuan ; Yang, Mingying ; Wang, Yan ; Wang, Philip L. ; Hu, Yinghe ; Tsien, Joe Z. / Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 35. pp. 12956-12960.
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