Electrochemical analysis of cell plasma membrane cholesterol at the airway surface of mouse trachea

Dechen Jiang, Danjun Fang, Thomas J. Kelley, James D. Burgess

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Electrochemical detection of plasma membrane cholesterol at the surface of excised mouse trachea tissue is reported. Cholesterol oxidase is covalently linked to an 11-mercaptoundecanoic acid submonolayer on the platinum electrode surface. The cholesterol oxidase-modified electrodes show steady-state responses for cholesterol in solution at physiological temperatures. Experiments for direct contact between the cholesterol oxidase-modified electrode and the surface of excised trachea tissue at 37°C indicate steady-state responses that are largely independent of the position of contact on the tissue surface. Tissue samples are mounted on a quartz crystal microbalance electrode to gauge contact force between the electrode and the tissue surface, and the steady-state electrode response for tissue cholesterol is shown to be largely independent of the contact force. Trachea tissue excised from a mouse model of cystic fibrosis, which is known to exhibit evaluated cholesterol in airway cells, shows an electrode response that is ∼40% larger than the response observed at wild-type mouse trachea tissue.

Original languageEnglish (US)
Pages (from-to)1235-1239
Number of pages5
JournalAnalytical Chemistry
Volume80
Issue number4
DOIs
StatePublished - Feb 15 2008

ASJC Scopus subject areas

  • Analytical Chemistry

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