Elevated interleukin-6 levels in peritoneal fluid of patients with pelvic pathology

R. P. Buyalos, V. A. Funari, Ricardo Azziz, J. M. Watson, O. Martinez-Maza

Research output: Contribution to journalArticle

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Abstract

Objective: To determine if interleukin 6 (IL-6) is a normal constituent of peritoneal fluid (PF), and if various types of pelvic pathology influence its presence within the PF microenvironment. Study Design: Peritoneal fluid from 73 women obtained at the time of laparoscopy was examined for the presence of IL-6 using an IL-6 specific sandwich enzyme-linked immunosorbent assay. Thirty-nine patients had pelvic endometriosis, 17 had nonendometriotic pelvic adhesive disease, and 17 subjects undergoing tubal sterilization without evidence of pelvic pathology served as controls. Results: Immunoreactive IL-6 was observed in the PF of all 73 subjects (range 0.26 to 11.16 ng/mL). The mean concentration of IL-6 was higher in women with nonendometriotic pelvic adhesions as compared with control subjects (1.28 ± 0.16 versus 0.80 ± 0.06 ng/mL, P < 0.03). There was no difference in the mean peritoneal concentrations of IL-6 between women with endometriosis (1.16 ± 0.28 ng/mL) and controls, P = 0.38. Twenty-seven of 73 patients (37%) demonstrated elevated levels (>1.0 ng/mL) of IL-6. Patients with pelvic adhesions were significantly more likely to have elevated concentrations of IL-6 than controls (10/17 [59%] versus 3/17 [18%], P < 0.02). Alternatively, the percentage of patients with elevated IL-6 concentrations did not differ between patients with endometriosis or controls (14/39 [36%] versus 3/17 [18%], P > 0.10). Conclusions: These findings demonstrate that IL-6 is a normal constituent of PF and that elevated levels are found in many patients with pelvic adhesions.

Original languageEnglish (US)
Pages (from-to)302-306
Number of pages5
JournalFertility and sterility
Volume58
Issue number2
StatePublished - Jan 1 1992

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Ascitic Fluid
Interleukin-6
Pathology
Tubal Sterilization
Endometriosis
Adhesives
Laparoscopy
Enzyme-Linked Immunosorbent Assay

Keywords

  • Cytokines
  • human peritoneal fluid
  • interleukin 6

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Buyalos, R. P., Funari, V. A., Azziz, R., Watson, J. M., & Martinez-Maza, O. (1992). Elevated interleukin-6 levels in peritoneal fluid of patients with pelvic pathology. Fertility and sterility, 58(2), 302-306.

Elevated interleukin-6 levels in peritoneal fluid of patients with pelvic pathology. / Buyalos, R. P.; Funari, V. A.; Azziz, Ricardo; Watson, J. M.; Martinez-Maza, O.

In: Fertility and sterility, Vol. 58, No. 2, 01.01.1992, p. 302-306.

Research output: Contribution to journalArticle

Buyalos, RP, Funari, VA, Azziz, R, Watson, JM & Martinez-Maza, O 1992, 'Elevated interleukin-6 levels in peritoneal fluid of patients with pelvic pathology', Fertility and sterility, vol. 58, no. 2, pp. 302-306.
Buyalos RP, Funari VA, Azziz R, Watson JM, Martinez-Maza O. Elevated interleukin-6 levels in peritoneal fluid of patients with pelvic pathology. Fertility and sterility. 1992 Jan 1;58(2):302-306.
Buyalos, R. P. ; Funari, V. A. ; Azziz, Ricardo ; Watson, J. M. ; Martinez-Maza, O. / Elevated interleukin-6 levels in peritoneal fluid of patients with pelvic pathology. In: Fertility and sterility. 1992 ; Vol. 58, No. 2. pp. 302-306.
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abstract = "Objective: To determine if interleukin 6 (IL-6) is a normal constituent of peritoneal fluid (PF), and if various types of pelvic pathology influence its presence within the PF microenvironment. Study Design: Peritoneal fluid from 73 women obtained at the time of laparoscopy was examined for the presence of IL-6 using an IL-6 specific sandwich enzyme-linked immunosorbent assay. Thirty-nine patients had pelvic endometriosis, 17 had nonendometriotic pelvic adhesive disease, and 17 subjects undergoing tubal sterilization without evidence of pelvic pathology served as controls. Results: Immunoreactive IL-6 was observed in the PF of all 73 subjects (range 0.26 to 11.16 ng/mL). The mean concentration of IL-6 was higher in women with nonendometriotic pelvic adhesions as compared with control subjects (1.28 ± 0.16 versus 0.80 ± 0.06 ng/mL, P < 0.03). There was no difference in the mean peritoneal concentrations of IL-6 between women with endometriosis (1.16 ± 0.28 ng/mL) and controls, P = 0.38. Twenty-seven of 73 patients (37{\%}) demonstrated elevated levels (>1.0 ng/mL) of IL-6. Patients with pelvic adhesions were significantly more likely to have elevated concentrations of IL-6 than controls (10/17 [59{\%}] versus 3/17 [18{\%}], P < 0.02). Alternatively, the percentage of patients with elevated IL-6 concentrations did not differ between patients with endometriosis or controls (14/39 [36{\%}] versus 3/17 [18{\%}], P > 0.10). Conclusions: These findings demonstrate that IL-6 is a normal constituent of PF and that elevated levels are found in many patients with pelvic adhesions.",
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AU - Watson, J. M.

AU - Martinez-Maza, O.

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N2 - Objective: To determine if interleukin 6 (IL-6) is a normal constituent of peritoneal fluid (PF), and if various types of pelvic pathology influence its presence within the PF microenvironment. Study Design: Peritoneal fluid from 73 women obtained at the time of laparoscopy was examined for the presence of IL-6 using an IL-6 specific sandwich enzyme-linked immunosorbent assay. Thirty-nine patients had pelvic endometriosis, 17 had nonendometriotic pelvic adhesive disease, and 17 subjects undergoing tubal sterilization without evidence of pelvic pathology served as controls. Results: Immunoreactive IL-6 was observed in the PF of all 73 subjects (range 0.26 to 11.16 ng/mL). The mean concentration of IL-6 was higher in women with nonendometriotic pelvic adhesions as compared with control subjects (1.28 ± 0.16 versus 0.80 ± 0.06 ng/mL, P < 0.03). There was no difference in the mean peritoneal concentrations of IL-6 between women with endometriosis (1.16 ± 0.28 ng/mL) and controls, P = 0.38. Twenty-seven of 73 patients (37%) demonstrated elevated levels (>1.0 ng/mL) of IL-6. Patients with pelvic adhesions were significantly more likely to have elevated concentrations of IL-6 than controls (10/17 [59%] versus 3/17 [18%], P < 0.02). Alternatively, the percentage of patients with elevated IL-6 concentrations did not differ between patients with endometriosis or controls (14/39 [36%] versus 3/17 [18%], P > 0.10). Conclusions: These findings demonstrate that IL-6 is a normal constituent of PF and that elevated levels are found in many patients with pelvic adhesions.

AB - Objective: To determine if interleukin 6 (IL-6) is a normal constituent of peritoneal fluid (PF), and if various types of pelvic pathology influence its presence within the PF microenvironment. Study Design: Peritoneal fluid from 73 women obtained at the time of laparoscopy was examined for the presence of IL-6 using an IL-6 specific sandwich enzyme-linked immunosorbent assay. Thirty-nine patients had pelvic endometriosis, 17 had nonendometriotic pelvic adhesive disease, and 17 subjects undergoing tubal sterilization without evidence of pelvic pathology served as controls. Results: Immunoreactive IL-6 was observed in the PF of all 73 subjects (range 0.26 to 11.16 ng/mL). The mean concentration of IL-6 was higher in women with nonendometriotic pelvic adhesions as compared with control subjects (1.28 ± 0.16 versus 0.80 ± 0.06 ng/mL, P < 0.03). There was no difference in the mean peritoneal concentrations of IL-6 between women with endometriosis (1.16 ± 0.28 ng/mL) and controls, P = 0.38. Twenty-seven of 73 patients (37%) demonstrated elevated levels (>1.0 ng/mL) of IL-6. Patients with pelvic adhesions were significantly more likely to have elevated concentrations of IL-6 than controls (10/17 [59%] versus 3/17 [18%], P < 0.02). Alternatively, the percentage of patients with elevated IL-6 concentrations did not differ between patients with endometriosis or controls (14/39 [36%] versus 3/17 [18%], P > 0.10). Conclusions: These findings demonstrate that IL-6 is a normal constituent of PF and that elevated levels are found in many patients with pelvic adhesions.

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