Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy

Robert K Yu; Theodore A.W. Koerner; J. Neel Scarsdale; James H. Prestegard

doi:10.1016/0009-3084(86)90041-1

Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy

Robert K Yu, Theodore A.W. Koerner, J. Neel Scarsdale, James H. Prestegard

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

The primary structure of the oligosaccharide moiety of a glycosphingolipid can be elucidated by employing high-field proton nuclear magnetic resonance (NMR) spectroscopy. Information with respect to the composition and configuration of its sugar residues, and the sequence and linkage sites of the oligosaccharide chain can be obtained by employing a variety of one- and two-dimensional techniques. The latter include both scalar and dipolar correlated two-dimensional NMR spectroscopy. These techniques are also useful in establishing the solution conformation (secondary structure) of the oligosaccharide moiety. Examples in utilizing these techniques in elucidating the primary and secondary structures of glycolipids are presented.

Original language	English (US)
Pages (from-to)	27-48
Number of pages	22
Journal	Chemistry and Physics of Lipids
Volume	42
Issue number	1-3
DOIs	https://doi.org/10.1016/0009-3084(86)90041-1
State	Published - Dec 15 1986
Externally published	Yes

Keywords

gangliosides
glycosphingolipids
proton NMR
secondary structure
solution conformation

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Organic Chemistry
Cell Biology

Access to Document

10.1016/0009-3084(86)90041-1

Cite this

@article{7f299c1bd2694c1a977bfb4b9544e588,

title = "Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy",

abstract = "The primary structure of the oligosaccharide moiety of a glycosphingolipid can be elucidated by employing high-field proton nuclear magnetic resonance (NMR) spectroscopy. Information with respect to the composition and configuration of its sugar residues, and the sequence and linkage sites of the oligosaccharide chain can be obtained by employing a variety of one- and two-dimensional techniques. The latter include both scalar and dipolar correlated two-dimensional NMR spectroscopy. These techniques are also useful in establishing the solution conformation (secondary structure) of the oligosaccharide moiety. Examples in utilizing these techniques in elucidating the primary and secondary structures of glycolipids are presented.",

keywords = "gangliosides, glycosphingolipids, proton NMR, secondary structure, solution conformation",

author = "Yu, {Robert K} and Koerner, {Theodore A.W.} and {Neel Scarsdale}, J. and Prestegard, {James H.}",

note = "Funding Information: This work was supportedb y USPHS grantsN S predoctorafel llowshipto J.N.S.",

year = "1986",

month = dec,

day = "15",

doi = "10.1016/0009-3084(86)90041-1",

language = "English (US)",

volume = "42",

pages = "27--48",

journal = "Chemistry and Physics of Lipids",

issn = "0009-3084",

publisher = "Elsevier Ireland Ltd",

number = "1-3",

}

TY - JOUR

T1 - Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy

AU - Yu, Robert K

AU - Koerner, Theodore A.W.

AU - Neel Scarsdale, J.

AU - Prestegard, James H.

N1 - Funding Information: This work was supportedb y USPHS grantsN S predoctorafel llowshipto J.N.S.

PY - 1986/12/15

Y1 - 1986/12/15

N2 - The primary structure of the oligosaccharide moiety of a glycosphingolipid can be elucidated by employing high-field proton nuclear magnetic resonance (NMR) spectroscopy. Information with respect to the composition and configuration of its sugar residues, and the sequence and linkage sites of the oligosaccharide chain can be obtained by employing a variety of one- and two-dimensional techniques. The latter include both scalar and dipolar correlated two-dimensional NMR spectroscopy. These techniques are also useful in establishing the solution conformation (secondary structure) of the oligosaccharide moiety. Examples in utilizing these techniques in elucidating the primary and secondary structures of glycolipids are presented.

AB - The primary structure of the oligosaccharide moiety of a glycosphingolipid can be elucidated by employing high-field proton nuclear magnetic resonance (NMR) spectroscopy. Information with respect to the composition and configuration of its sugar residues, and the sequence and linkage sites of the oligosaccharide chain can be obtained by employing a variety of one- and two-dimensional techniques. The latter include both scalar and dipolar correlated two-dimensional NMR spectroscopy. These techniques are also useful in establishing the solution conformation (secondary structure) of the oligosaccharide moiety. Examples in utilizing these techniques in elucidating the primary and secondary structures of glycolipids are presented.

KW - gangliosides

KW - glycosphingolipids

KW - proton NMR

KW - secondary structure

KW - solution conformation

UR - http://www.scopus.com/inward/record.url?scp=0023050960&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023050960&partnerID=8YFLogxK

U2 - 10.1016/0009-3084(86)90041-1

DO - 10.1016/0009-3084(86)90041-1

M3 - Article

C2 - 3549018

AN - SCOPUS:0023050960

SN - 0009-3084

VL - 42

SP - 27

EP - 48

JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

IS - 1-3

ER -

Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this