Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy

Robert K Yu, Theodore A.W. Koerner, J. Neel Scarsdale, James H. Prestegard

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The primary structure of the oligosaccharide moiety of a glycosphingolipid can be elucidated by employing high-field proton nuclear magnetic resonance (NMR) spectroscopy. Information with respect to the composition and configuration of its sugar residues, and the sequence and linkage sites of the oligosaccharide chain can be obtained by employing a variety of one- and two-dimensional techniques. The latter include both scalar and dipolar correlated two-dimensional NMR spectroscopy. These techniques are also useful in establishing the solution conformation (secondary structure) of the oligosaccharide moiety. Examples in utilizing these techniques in elucidating the primary and secondary structures of glycolipids are presented.

Original languageEnglish (US)
Pages (from-to)27-48
Number of pages22
JournalChemistry and Physics of Lipids
Volume42
Issue number1-3
DOIs
StatePublished - Dec 15 1986
Externally publishedYes

Keywords

  • gangliosides
  • glycosphingolipids
  • proton NMR
  • secondary structure
  • solution conformation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Cell Biology

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