Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy

Robert K Yu, Theodore A.W. Koerner, J. Neel Scarsdale, James H. Prestegard

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The primary structure of the oligosaccharide moiety of a glycosphingolipid can be elucidated by employing high-field proton nuclear magnetic resonance (NMR) spectroscopy. Information with respect to the composition and configuration of its sugar residues, and the sequence and linkage sites of the oligosaccharide chain can be obtained by employing a variety of one- and two-dimensional techniques. The latter include both scalar and dipolar correlated two-dimensional NMR spectroscopy. These techniques are also useful in establishing the solution conformation (secondary structure) of the oligosaccharide moiety. Examples in utilizing these techniques in elucidating the primary and secondary structures of glycolipids are presented.

Original languageEnglish (US)
Pages (from-to)27-48
Number of pages22
JournalChemistry and Physics of Lipids
Volume42
Issue number1-3
DOIs
StatePublished - Dec 15 1986
Externally publishedYes

Fingerprint

Glycolipids
Oligosaccharides
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Glycosphingolipids
Sugars
Conformations
Chemical analysis
Proton Magnetic Resonance Spectroscopy

Keywords

  • gangliosides
  • glycosphingolipids
  • proton NMR
  • secondary structure
  • solution conformation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Cell Biology

Cite this

Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy. / Yu, Robert K; Koerner, Theodore A.W.; Neel Scarsdale, J.; Prestegard, James H.

In: Chemistry and Physics of Lipids, Vol. 42, No. 1-3, 15.12.1986, p. 27-48.

Research output: Contribution to journalArticle

Yu, Robert K ; Koerner, Theodore A.W. ; Neel Scarsdale, J. ; Prestegard, James H. / Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy. In: Chemistry and Physics of Lipids. 1986 ; Vol. 42, No. 1-3. pp. 27-48.
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