Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages

Carol W. Wuenschell, Mary E. Sunday, Gurmukh Singh, Parviz Minoo, Harold C. Slavkin, David Warburton

Research output: Contribution to journalArticle

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Abstract

Developmental expression of market genes representative of different mature cell types can be used to study differentiation of cell lineages. We used immunohistochemistry to study expression in developing mouse lung of calcitonin gene-related peptide (CGRP), Clara cell 10-KD protein (CC10), and surfactant protein-A (SP-A), markers that are differentially expressed in neuroendocrine cells, Clara cells, and Type II alveolar cells. Two distinct developmental phases were revealed. The earlier phase (embryonic days 13-15; E13-E15) was characterized by CGRP, CC10s and SP-A immunostaining in all epithelial cells of the distal airways, with the three patterns being virtually identical in adjacent sections. The later phase (E16-E18) was characterized by emergence of staining of the differentiated cell types. These expression patterns were recapitulated in serumless organ culture, demonstrating that information necessary to generate both phases of gene expression is present within the lung anlage by E11. We conclude that CGRP, CC10, and SP-A are co-expressed in most or all cells of the distal lung epithelium at E13-E15 and later become restricted to different cell lineages. This transient expression in progenitor cells of gene products characteristic of diverse differentiated cell types may reflect an underlying mechanism of gene regulation.

Original languageEnglish (US)
Pages (from-to)113-123
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume44
Issue number2
DOIs
StatePublished - Jan 1 1996

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Cell Lineage
Stem Cells
Epithelial Cells
Lung
Pulmonary Surfactant-Associated Protein A
Calcitonin Gene-Related Peptide
Alveolar Epithelial Cells
Gene Expression
Neuroendocrine Cells
Organ Culture Techniques
Genes
Proteins
Epithelium
Immunohistochemistry
Staining and Labeling

Keywords

  • Cell lineage
  • Clara cells
  • Epithelial progenitors
  • Gene expression
  • Immunohistochemistry
  • Lung development
  • Mouse
  • Pulmonary neuroendocrine cells
  • Type II alveolar epithelial cells

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages. / Wuenschell, Carol W.; Sunday, Mary E.; Singh, Gurmukh; Minoo, Parviz; Slavkin, Harold C.; Warburton, David.

In: Journal of Histochemistry and Cytochemistry, Vol. 44, No. 2, 01.01.1996, p. 113-123.

Research output: Contribution to journalArticle

Wuenschell, Carol W. ; Sunday, Mary E. ; Singh, Gurmukh ; Minoo, Parviz ; Slavkin, Harold C. ; Warburton, David. / Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages. In: Journal of Histochemistry and Cytochemistry. 1996 ; Vol. 44, No. 2. pp. 113-123.
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