EMR-3: A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target

Ari J. Kane, Michael E. Sughrue, Martin J. Rutkowski, Joanna J. Phillips, Andrew T. Parsa

Research output: Contribution to journalArticle

Abstract

Epidermal growth factor module-containing mucin-like hormone receptor-3 (EMR-3) is a G-protein coupled receptor with unknown ligand and cellular function. Upregulation of EMR-3 in glioblastoma (GBM) multiforme is associated with poor survival. We investigated the expression patterns and functional significance of EMR-3 in GBM using immunohistochemistry, western blot, reverse transcription PCR, and small interfering RNA knockdown in proliferation and invasion assays. EMR-3 is variably expressed in primary human GBM tissues and cell lines. Knocking down EMR-3 has no impact on cellular proliferation, but decreases cellular invasion by greater than 3-fold. EMR-3 is a potential mediator of cellular invasion in GBM. Given the poor survival associated with high levels of EMR-3 expression in glioma patients, our results provide impetus to explore EMR-3 as a potential therapeutic target.

Original languageEnglish (US)
Pages (from-to)1018-1022
Number of pages5
JournalNeuroReport
Volume21
Issue number16
DOIs
StatePublished - Nov 17 2010
Externally publishedYes

Fingerprint

Glioblastoma
Survival
Mucins
Therapeutics
G-Protein-Coupled Receptors
Epidermal Growth Factor
Glioma
Small Interfering RNA
Reverse Transcription
Up-Regulation
Western Blotting
Immunohistochemistry
Cell Proliferation
Hormones
Ligands
Cell Line
Polymerase Chain Reaction

Keywords

  • EGF module containing mucin-like hormone receptor-3
  • glioma
  • invasion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kane, A. J., Sughrue, M. E., Rutkowski, M. J., Phillips, J. J., & Parsa, A. T. (2010). EMR-3: A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target. NeuroReport, 21(16), 1018-1022. https://doi.org/10.1097/WNR.0b013e32833f19f2

EMR-3 : A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target. / Kane, Ari J.; Sughrue, Michael E.; Rutkowski, Martin J.; Phillips, Joanna J.; Parsa, Andrew T.

In: NeuroReport, Vol. 21, No. 16, 17.11.2010, p. 1018-1022.

Research output: Contribution to journalArticle

Kane, AJ, Sughrue, ME, Rutkowski, MJ, Phillips, JJ & Parsa, AT 2010, 'EMR-3: A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target', NeuroReport, vol. 21, no. 16, pp. 1018-1022. https://doi.org/10.1097/WNR.0b013e32833f19f2
Kane, Ari J. ; Sughrue, Michael E. ; Rutkowski, Martin J. ; Phillips, Joanna J. ; Parsa, Andrew T. / EMR-3 : A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target. In: NeuroReport. 2010 ; Vol. 21, No. 16. pp. 1018-1022.
@article{ab436701fe544a8c9049a1dbf804a9a7,
title = "EMR-3: A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target",
abstract = "Epidermal growth factor module-containing mucin-like hormone receptor-3 (EMR-3) is a G-protein coupled receptor with unknown ligand and cellular function. Upregulation of EMR-3 in glioblastoma (GBM) multiforme is associated with poor survival. We investigated the expression patterns and functional significance of EMR-3 in GBM using immunohistochemistry, western blot, reverse transcription PCR, and small interfering RNA knockdown in proliferation and invasion assays. EMR-3 is variably expressed in primary human GBM tissues and cell lines. Knocking down EMR-3 has no impact on cellular proliferation, but decreases cellular invasion by greater than 3-fold. EMR-3 is a potential mediator of cellular invasion in GBM. Given the poor survival associated with high levels of EMR-3 expression in glioma patients, our results provide impetus to explore EMR-3 as a potential therapeutic target.",
keywords = "EGF module containing mucin-like hormone receptor-3, glioma, invasion",
author = "Kane, {Ari J.} and Sughrue, {Michael E.} and Rutkowski, {Martin J.} and Phillips, {Joanna J.} and Parsa, {Andrew T.}",
year = "2010",
month = "11",
day = "17",
doi = "10.1097/WNR.0b013e32833f19f2",
language = "English (US)",
volume = "21",
pages = "1018--1022",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "16",

}

TY - JOUR

T1 - EMR-3

T2 - A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target

AU - Kane, Ari J.

AU - Sughrue, Michael E.

AU - Rutkowski, Martin J.

AU - Phillips, Joanna J.

AU - Parsa, Andrew T.

PY - 2010/11/17

Y1 - 2010/11/17

N2 - Epidermal growth factor module-containing mucin-like hormone receptor-3 (EMR-3) is a G-protein coupled receptor with unknown ligand and cellular function. Upregulation of EMR-3 in glioblastoma (GBM) multiforme is associated with poor survival. We investigated the expression patterns and functional significance of EMR-3 in GBM using immunohistochemistry, western blot, reverse transcription PCR, and small interfering RNA knockdown in proliferation and invasion assays. EMR-3 is variably expressed in primary human GBM tissues and cell lines. Knocking down EMR-3 has no impact on cellular proliferation, but decreases cellular invasion by greater than 3-fold. EMR-3 is a potential mediator of cellular invasion in GBM. Given the poor survival associated with high levels of EMR-3 expression in glioma patients, our results provide impetus to explore EMR-3 as a potential therapeutic target.

AB - Epidermal growth factor module-containing mucin-like hormone receptor-3 (EMR-3) is a G-protein coupled receptor with unknown ligand and cellular function. Upregulation of EMR-3 in glioblastoma (GBM) multiforme is associated with poor survival. We investigated the expression patterns and functional significance of EMR-3 in GBM using immunohistochemistry, western blot, reverse transcription PCR, and small interfering RNA knockdown in proliferation and invasion assays. EMR-3 is variably expressed in primary human GBM tissues and cell lines. Knocking down EMR-3 has no impact on cellular proliferation, but decreases cellular invasion by greater than 3-fold. EMR-3 is a potential mediator of cellular invasion in GBM. Given the poor survival associated with high levels of EMR-3 expression in glioma patients, our results provide impetus to explore EMR-3 as a potential therapeutic target.

KW - EGF module containing mucin-like hormone receptor-3

KW - glioma

KW - invasion

UR - http://www.scopus.com/inward/record.url?scp=78049288580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049288580&partnerID=8YFLogxK

U2 - 10.1097/WNR.0b013e32833f19f2

DO - 10.1097/WNR.0b013e32833f19f2

M3 - Article

C2 - 20827226

AN - SCOPUS:78049288580

VL - 21

SP - 1018

EP - 1022

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 16

ER -