TY - JOUR
T1 - Enamel remineralization via poly(amido amine) and adhesive resin containing calcium phosphate nanoparticles
AU - Gao, Yuan
AU - Liang, Kunneng
AU - Weir, Michael D.
AU - Gao, Jianghong
AU - Imazato, Satoshi
AU - Tay, Franklin R.
AU - Lynch, Christopher D.
AU - Oates, Thomas W.
AU - Li, Jiyao
AU - Xu, Hockin H.K.
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/1
Y1 - 2020/1
N2 - Objectives: The objective of this study was to investigate enamel remineralization using salivary statherin protein-inspired poly(amidoamine) dendrimer (SN15-PAMAM) and adhesive containing nanoparticles of amorphous calcium phosphate (NACP) in a cyclic artificial saliva/demineralizing solution for the first time. Methods: The enamel shear bond strengths of NACP adhesives were measured compared to commercial adhesive (Scotchbond Multi-Purpose, 3 M). Adhesive disks containing NACP were tested for calcium (Ca) and phosphorus (P) ions release. Four groups were tested: (1) enamel control, (2) enamel with NACP, (3) enamel with SN15-PAMAM, and (4) enamel with SN15-PAMAM + NACP. The specimens were treated with cyclic artificial saliva/demineralizing solution for 28 days. The remineralized enamel specimens were examined by surface and cross-sectional hardness test. Results: NACP adhesive yielded a similar shear bond strength to commercial control (Scotchbond Multi-Purpose, 3 M). NACP adhesive released high levels of Ca and P ions. At 28 days, the enamel hardness of SN15-PAMAM + NACP group was 2.89 ± 0.13 GPa, significantly higher than that of control group (1.46 ± 0.10 GPa) (p < 0.05). SN15-PAMAM + NACP increased the enamel cross-sectional hardness at 28 days; at 25 μm, enamel cross-sectional hardness was 90 % higher than that of control group (p < 0.05). Significance: The novel SN15-PAMAM + NACP adhesive method could achieve 90 % higher enamel remineralization of the artificial caries than the control under acid challenge for the first time. This method is promising for use after tooth cavity preparation, or as a coating on enamel with white spot lesions (WSLs) for prevention, to reduce secondary caries, prevent caries procession and protect tooth structures.
AB - Objectives: The objective of this study was to investigate enamel remineralization using salivary statherin protein-inspired poly(amidoamine) dendrimer (SN15-PAMAM) and adhesive containing nanoparticles of amorphous calcium phosphate (NACP) in a cyclic artificial saliva/demineralizing solution for the first time. Methods: The enamel shear bond strengths of NACP adhesives were measured compared to commercial adhesive (Scotchbond Multi-Purpose, 3 M). Adhesive disks containing NACP were tested for calcium (Ca) and phosphorus (P) ions release. Four groups were tested: (1) enamel control, (2) enamel with NACP, (3) enamel with SN15-PAMAM, and (4) enamel with SN15-PAMAM + NACP. The specimens were treated with cyclic artificial saliva/demineralizing solution for 28 days. The remineralized enamel specimens were examined by surface and cross-sectional hardness test. Results: NACP adhesive yielded a similar shear bond strength to commercial control (Scotchbond Multi-Purpose, 3 M). NACP adhesive released high levels of Ca and P ions. At 28 days, the enamel hardness of SN15-PAMAM + NACP group was 2.89 ± 0.13 GPa, significantly higher than that of control group (1.46 ± 0.10 GPa) (p < 0.05). SN15-PAMAM + NACP increased the enamel cross-sectional hardness at 28 days; at 25 μm, enamel cross-sectional hardness was 90 % higher than that of control group (p < 0.05). Significance: The novel SN15-PAMAM + NACP adhesive method could achieve 90 % higher enamel remineralization of the artificial caries than the control under acid challenge for the first time. This method is promising for use after tooth cavity preparation, or as a coating on enamel with white spot lesions (WSLs) for prevention, to reduce secondary caries, prevent caries procession and protect tooth structures.
KW - Adhesive
KW - Enamel hardness
KW - Enamel remineralization
KW - Nano-calcium phosphate
KW - Statherin protein poly(amidoamine) dendrimer
KW - pH cycling
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U2 - 10.1016/j.jdent.2019.103262
DO - 10.1016/j.jdent.2019.103262
M3 - Article
C2 - 31837358
AN - SCOPUS:85076998850
SN - 0300-5712
VL - 92
JO - Journal of Dentistry
JF - Journal of Dentistry
M1 - 103262
ER -