Endogenous enzymatic activity of primary and permanent dentine

Tatjana Maravic, Lorenzo Breschi, Federica Paganelli, Giulio Alessandri Bonetti, Stefano Martina, Gianni Di Giorgio, Maurizio Bossù, Antonella Polimeni, Vittorio Checchi, Luigi Generali, Franklin R. Tay, Milena Cadenaro, Annalisa Mazzoni

Research output: Contribution to journalArticlepeer-review

Abstract

Matrix metalloproteinases (MMPs) play an important role in tooth development and influence caries development and hybrid layer degradation. Literature is scant on the differences in the activity of MMPs between primary and permanent dentine. Accordingly, the aim of the present study was to investigate endogenous gelatinolytic activity in primary and permanent dentine. Separate batches of dentine powder were obtained from intact human primary and permanent molars (n = 6). Each batch was divided in two subgroups: (1) mineralised; and (2) demineralised with 10% H3PO4 . After protein extraction, gelatine zymography was performed. Furthermore, in situ zymography was performed on dentine sections of the same groups (n = 3). The slices were polished, covered with fluorescein-conjugated gelatine and evaluated using a confocal microscope. In situ zymography data were analysed using two-way analysis of variance and post hoc Holm– Šidák statistics (α = 0.05). Primary dentine showed poorly defined bands in the zymograms that vaguely corresponded to the pro-form and active form of MMP-2 and the pro-form of MMP-9. In permanent dentine, demineralised powder demonstrated stronger gelatinolytic activity than mineralised powder. In situ zymography identified stronger enzymatic activity in primary etched dentine (p < 0.05). Stronger enzymatic activity recorded in primary dentine may be related to the differences in morphology and composition between primary and permanent dentine.

Original languageEnglish (US)
Article number4043
JournalMaterials
Volume14
Issue number14
DOIs
StatePublished - Jul 2 2021

Keywords

  • MMPs
  • Permanent dentine
  • Primary dentine
  • Zymography

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

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