Endogenous Phosphorylation of a 61,000 Dalton Hippocampal Protein Increases Following Traumatic Brain Injury

Keyi Yang, William C. Taft, C. Edward Dixon, Robert K. yu, Ronald L. Hayes

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Acute biochemical consequences of moderate traumatic brain injury (TBI) include activation of kinases, including protein kinase C (PKC). To determine the possible consequences of PKC activation at the substrate level, we have examined protein phosphorylation patterns 1 h following injury. Although the phosphorylation of most proteins remained unchanged following injury, we observed a significant increase in the phosphorylation of a 61,000 dalton protein (TBI61) in injured rat hippocampus (121 % higher than sham control) in vitro. TBI61 phosphorylation could be enhanced by phosphatidyl serine and diacylglycerol or by addition of exogenous PKC. In addition, TBI61 phosphorylation was inhibited by the PKC inhibitor, staurosporine, suggesting further that this protein may be a PKC substrate. These data suggest that TBI increases the phosphorylation of a 61 kD hippocampal protein in vitro. Increases in the protein level and activity of PKC could contribute to this increased phosphorylation.

Original languageEnglish (US)
Pages (from-to)523-532
Number of pages10
JournalJournal of Neurotrauma
Volume11
Issue number5
DOIs
StatePublished - Oct 1994

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Phosphorylation
Protein Kinase C
Proteins
Staurosporine
Protein C Inhibitor
Phosphatidylserines
Diglycerides
Wounds and Injuries
Protein Kinase Inhibitors
Traumatic Brain Injury
Hippocampus
In Vitro Techniques

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Endogenous Phosphorylation of a 61,000 Dalton Hippocampal Protein Increases Following Traumatic Brain Injury. / Yang, Keyi; Taft, William C.; Dixon, C. Edward; yu, Robert K.; Hayes, Ronald L.

In: Journal of Neurotrauma, Vol. 11, No. 5, 10.1994, p. 523-532.

Research output: Contribution to journalArticle

Yang, Keyi ; Taft, William C. ; Dixon, C. Edward ; yu, Robert K. ; Hayes, Ronald L. / Endogenous Phosphorylation of a 61,000 Dalton Hippocampal Protein Increases Following Traumatic Brain Injury. In: Journal of Neurotrauma. 1994 ; Vol. 11, No. 5. pp. 523-532.
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