Endometrial morphology and modulation of hormone receptors during ovarian stimulation for assisted reproductive technology cycles

Laura Detti, Ghassan M. Saed, Nicole M. Fletcher, Michael L. Kruger, Michelle Brossoit, Michael Peter Diamond

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: To study the biochemical and morphologic implications of different hormonal levels on endometrial development during assisted reproductive technology (ART) cycles. Design: Prospective, controlled study. Setting: University center. Patient(s): Eleven women during a natural cycle (controls) and 11 oocyte donors during an ART cycle (treated). Intervention(s): At the time consistent with day-3 ET, a transvaginal ultrasound, an endometrial biopsy, and blood sampling were performed. Morphology and thickness of the endometrial stripe were recorded. Real-time reverse transcriptase-polymerase chain reaction was used to measure messenger RNA (mRNA) levels for estrogen receptor (ER)-α, ER-β, P receptor (PR)-A, and PR-B in the endometrial tissue. Main Outcome Measure(s): To evaluate morphologic and biochemical endometrial development. Result(s): Endometrium was mostly trilaminar (proliferative-like pattern) and thicker in the treated group, as opposed to homogeneous and thinner in the controls. The PR-B mRNA expression increased 41% in treated patients; PR-A mRNA expression, instead, was unchanged. Serum E 2 and P were higher in the treated group than in controls. In contrast, FSH and LH levels were lower in the treated group. Conclusion(s): When compared with natural cycles, exposure of the endometrium to high hormone levels during ovarian stimulation significantly increased PR-B receptor expression at the time of ET. Concurrently, a proliferative-like endometrial pattern persisted. These findings reflect a delayed endometrial development in ART.

Original languageEnglish (US)
Pages (from-to)1037-1041
Number of pages5
JournalFertility and sterility
Volume95
Issue number3
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

Fingerprint

Assisted Reproductive Techniques
Ovulation Induction
Hormones
Endometrium
Estrogen Receptors
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Oocytes
Real-Time Polymerase Chain Reaction
Outcome Assessment (Health Care)
Tissue Donors
Prospective Studies
Biopsy
Control Groups
Serum

Keywords

  • ART
  • Endometrial development
  • GnRH antagonist
  • hormone receptors
  • real-time RT-PCR
  • ultrasound

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Endometrial morphology and modulation of hormone receptors during ovarian stimulation for assisted reproductive technology cycles. / Detti, Laura; Saed, Ghassan M.; Fletcher, Nicole M.; Kruger, Michael L.; Brossoit, Michelle; Diamond, Michael Peter.

In: Fertility and sterility, Vol. 95, No. 3, 01.03.2011, p. 1037-1041.

Research output: Contribution to journalArticle

Detti, Laura ; Saed, Ghassan M. ; Fletcher, Nicole M. ; Kruger, Michael L. ; Brossoit, Michelle ; Diamond, Michael Peter. / Endometrial morphology and modulation of hormone receptors during ovarian stimulation for assisted reproductive technology cycles. In: Fertility and sterility. 2011 ; Vol. 95, No. 3. pp. 1037-1041.
@article{058fa8a5cf634b8eb0f6c9ea21552210,
title = "Endometrial morphology and modulation of hormone receptors during ovarian stimulation for assisted reproductive technology cycles",
abstract = "Objective: To study the biochemical and morphologic implications of different hormonal levels on endometrial development during assisted reproductive technology (ART) cycles. Design: Prospective, controlled study. Setting: University center. Patient(s): Eleven women during a natural cycle (controls) and 11 oocyte donors during an ART cycle (treated). Intervention(s): At the time consistent with day-3 ET, a transvaginal ultrasound, an endometrial biopsy, and blood sampling were performed. Morphology and thickness of the endometrial stripe were recorded. Real-time reverse transcriptase-polymerase chain reaction was used to measure messenger RNA (mRNA) levels for estrogen receptor (ER)-α, ER-β, P receptor (PR)-A, and PR-B in the endometrial tissue. Main Outcome Measure(s): To evaluate morphologic and biochemical endometrial development. Result(s): Endometrium was mostly trilaminar (proliferative-like pattern) and thicker in the treated group, as opposed to homogeneous and thinner in the controls. The PR-B mRNA expression increased 41{\%} in treated patients; PR-A mRNA expression, instead, was unchanged. Serum E 2 and P were higher in the treated group than in controls. In contrast, FSH and LH levels were lower in the treated group. Conclusion(s): When compared with natural cycles, exposure of the endometrium to high hormone levels during ovarian stimulation significantly increased PR-B receptor expression at the time of ET. Concurrently, a proliferative-like endometrial pattern persisted. These findings reflect a delayed endometrial development in ART.",
keywords = "ART, Endometrial development, GnRH antagonist, hormone receptors, real-time RT-PCR, ultrasound",
author = "Laura Detti and Saed, {Ghassan M.} and Fletcher, {Nicole M.} and Kruger, {Michael L.} and Michelle Brossoit and Diamond, {Michael Peter}",
year = "2011",
month = "3",
day = "1",
doi = "10.1016/j.fertnstert.2010.12.025",
language = "English (US)",
volume = "95",
pages = "1037--1041",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Endometrial morphology and modulation of hormone receptors during ovarian stimulation for assisted reproductive technology cycles

AU - Detti, Laura

AU - Saed, Ghassan M.

AU - Fletcher, Nicole M.

AU - Kruger, Michael L.

AU - Brossoit, Michelle

AU - Diamond, Michael Peter

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Objective: To study the biochemical and morphologic implications of different hormonal levels on endometrial development during assisted reproductive technology (ART) cycles. Design: Prospective, controlled study. Setting: University center. Patient(s): Eleven women during a natural cycle (controls) and 11 oocyte donors during an ART cycle (treated). Intervention(s): At the time consistent with day-3 ET, a transvaginal ultrasound, an endometrial biopsy, and blood sampling were performed. Morphology and thickness of the endometrial stripe were recorded. Real-time reverse transcriptase-polymerase chain reaction was used to measure messenger RNA (mRNA) levels for estrogen receptor (ER)-α, ER-β, P receptor (PR)-A, and PR-B in the endometrial tissue. Main Outcome Measure(s): To evaluate morphologic and biochemical endometrial development. Result(s): Endometrium was mostly trilaminar (proliferative-like pattern) and thicker in the treated group, as opposed to homogeneous and thinner in the controls. The PR-B mRNA expression increased 41% in treated patients; PR-A mRNA expression, instead, was unchanged. Serum E 2 and P were higher in the treated group than in controls. In contrast, FSH and LH levels were lower in the treated group. Conclusion(s): When compared with natural cycles, exposure of the endometrium to high hormone levels during ovarian stimulation significantly increased PR-B receptor expression at the time of ET. Concurrently, a proliferative-like endometrial pattern persisted. These findings reflect a delayed endometrial development in ART.

AB - Objective: To study the biochemical and morphologic implications of different hormonal levels on endometrial development during assisted reproductive technology (ART) cycles. Design: Prospective, controlled study. Setting: University center. Patient(s): Eleven women during a natural cycle (controls) and 11 oocyte donors during an ART cycle (treated). Intervention(s): At the time consistent with day-3 ET, a transvaginal ultrasound, an endometrial biopsy, and blood sampling were performed. Morphology and thickness of the endometrial stripe were recorded. Real-time reverse transcriptase-polymerase chain reaction was used to measure messenger RNA (mRNA) levels for estrogen receptor (ER)-α, ER-β, P receptor (PR)-A, and PR-B in the endometrial tissue. Main Outcome Measure(s): To evaluate morphologic and biochemical endometrial development. Result(s): Endometrium was mostly trilaminar (proliferative-like pattern) and thicker in the treated group, as opposed to homogeneous and thinner in the controls. The PR-B mRNA expression increased 41% in treated patients; PR-A mRNA expression, instead, was unchanged. Serum E 2 and P were higher in the treated group than in controls. In contrast, FSH and LH levels were lower in the treated group. Conclusion(s): When compared with natural cycles, exposure of the endometrium to high hormone levels during ovarian stimulation significantly increased PR-B receptor expression at the time of ET. Concurrently, a proliferative-like endometrial pattern persisted. These findings reflect a delayed endometrial development in ART.

KW - ART

KW - Endometrial development

KW - GnRH antagonist

KW - hormone receptors

KW - real-time RT-PCR

KW - ultrasound

UR - http://www.scopus.com/inward/record.url?scp=79951957945&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951957945&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2010.12.025

DO - 10.1016/j.fertnstert.2010.12.025

M3 - Article

VL - 95

SP - 1037

EP - 1041

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 3

ER -