Abstract
Background: We have investigated the role of L-arginine in hyperhomocysteinemia (HHCY). L-arginine is the substrate required for NO production by endothelial NOS (eNOS). When L-arginine is limited, NOS acts principally upon O2 to form superoxide (O2·-). Because HHCY causes formation of reactive oxygen species and reduced endothelial-dependent vasodilation, we hypothesized that HHCY decreases NO formation by limiting the cellular supply of L-arginine. Material and Methods: Studies with cultured bovine aortic endothelial cells (ECs) determined effects of HCY on transport of [3H] L-arginine. Effects on L-arginine transporter protein CAT-1 and eNOS protein were assessed by immunoblotting. Peroxynitrite formation was evaluated by an immunoassay for nitrotyrosine levels. eNOS activity in forming NO was determined by assay for 3H-L-arginine to 3H-citrulline conversion. Results: HCY had a depressive effect on arginine transport in ECs. HCY treatment for a 24 hr period decreased arginine uptake by 27%. HCY treatment for 24 hr significantly reduced cellular levels of the CAT-1 arginine transporter protein (∼30%) and increased nitrotyrosine formation, whereas levels of eNOS protein and basal NOS activity were not altered. Nevertheless, total NO production as indicated by citrulline conversion was significantly decreased. Treatment with the antioxidant N-acetylcysteine reversed the HCY effect on arginine transport, suggesting that transporter oxidation may contribute to the endothelial dysfunction. Conclusion: The association of HCY-induced decreases in NO formation with decreases in function and expression of the arginine transporter in the absence of alterations in eNOS expression or activity suggests a primary role for arginine transport alterations in HHCY. The action of HCY to reduce arginine uptake may accentuate endothelial dysfunction due to generation of O2·- and peroxynitrite formation, which may cause further oxidative injury.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 585-591 |
| Number of pages | 7 |
| Journal | Medical Science Monitor |
| Volume | 7 |
| Issue number | 4 |
| State | Published - Aug 9 2001 |
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Keywords
- Endothelial cell dysfunction
- Hyperhomocysteinemia
- L-arginine uptake
- Nitric oxide synthase
- Oxidative stress
ASJC Scopus subject areas
- Medicine(all)
Cite this
Endothelial cell dysfunction in a model of oxidative stress. / Jin, Liming; Abou-Mohamed, Gamal; Caldwell, Ruth B.; Caldwell, R. William.
In: Medical Science Monitor, Vol. 7, No. 4, 09.08.2001, p. 585-591.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endothelial cell dysfunction in a model of oxidative stress
AU - Jin, Liming
AU - Abou-Mohamed, Gamal
AU - Caldwell, Ruth B.
AU - Caldwell, R. William
PY - 2001/8/9
Y1 - 2001/8/9
N2 - Background: We have investigated the role of L-arginine in hyperhomocysteinemia (HHCY). L-arginine is the substrate required for NO production by endothelial NOS (eNOS). When L-arginine is limited, NOS acts principally upon O2 to form superoxide (O2·-). Because HHCY causes formation of reactive oxygen species and reduced endothelial-dependent vasodilation, we hypothesized that HHCY decreases NO formation by limiting the cellular supply of L-arginine. Material and Methods: Studies with cultured bovine aortic endothelial cells (ECs) determined effects of HCY on transport of [3H] L-arginine. Effects on L-arginine transporter protein CAT-1 and eNOS protein were assessed by immunoblotting. Peroxynitrite formation was evaluated by an immunoassay for nitrotyrosine levels. eNOS activity in forming NO was determined by assay for 3H-L-arginine to 3H-citrulline conversion. Results: HCY had a depressive effect on arginine transport in ECs. HCY treatment for a 24 hr period decreased arginine uptake by 27%. HCY treatment for 24 hr significantly reduced cellular levels of the CAT-1 arginine transporter protein (∼30%) and increased nitrotyrosine formation, whereas levels of eNOS protein and basal NOS activity were not altered. Nevertheless, total NO production as indicated by citrulline conversion was significantly decreased. Treatment with the antioxidant N-acetylcysteine reversed the HCY effect on arginine transport, suggesting that transporter oxidation may contribute to the endothelial dysfunction. Conclusion: The association of HCY-induced decreases in NO formation with decreases in function and expression of the arginine transporter in the absence of alterations in eNOS expression or activity suggests a primary role for arginine transport alterations in HHCY. The action of HCY to reduce arginine uptake may accentuate endothelial dysfunction due to generation of O2·- and peroxynitrite formation, which may cause further oxidative injury.
AB - Background: We have investigated the role of L-arginine in hyperhomocysteinemia (HHCY). L-arginine is the substrate required for NO production by endothelial NOS (eNOS). When L-arginine is limited, NOS acts principally upon O2 to form superoxide (O2·-). Because HHCY causes formation of reactive oxygen species and reduced endothelial-dependent vasodilation, we hypothesized that HHCY decreases NO formation by limiting the cellular supply of L-arginine. Material and Methods: Studies with cultured bovine aortic endothelial cells (ECs) determined effects of HCY on transport of [3H] L-arginine. Effects on L-arginine transporter protein CAT-1 and eNOS protein were assessed by immunoblotting. Peroxynitrite formation was evaluated by an immunoassay for nitrotyrosine levels. eNOS activity in forming NO was determined by assay for 3H-L-arginine to 3H-citrulline conversion. Results: HCY had a depressive effect on arginine transport in ECs. HCY treatment for a 24 hr period decreased arginine uptake by 27%. HCY treatment for 24 hr significantly reduced cellular levels of the CAT-1 arginine transporter protein (∼30%) and increased nitrotyrosine formation, whereas levels of eNOS protein and basal NOS activity were not altered. Nevertheless, total NO production as indicated by citrulline conversion was significantly decreased. Treatment with the antioxidant N-acetylcysteine reversed the HCY effect on arginine transport, suggesting that transporter oxidation may contribute to the endothelial dysfunction. Conclusion: The association of HCY-induced decreases in NO formation with decreases in function and expression of the arginine transporter in the absence of alterations in eNOS expression or activity suggests a primary role for arginine transport alterations in HHCY. The action of HCY to reduce arginine uptake may accentuate endothelial dysfunction due to generation of O2·- and peroxynitrite formation, which may cause further oxidative injury.
KW - Endothelial cell dysfunction
KW - Hyperhomocysteinemia
KW - L-arginine uptake
KW - Nitric oxide synthase
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=0034928371&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034928371&partnerID=8YFLogxK
M3 - Article
C2 - 11433181
AN - SCOPUS:0034928371
VL - 7
SP - 585
EP - 591
JO - Medical Science Monitor
JF - Medical Science Monitor
SN - 1234-1010
IS - 4
ER -