Endothelin-1 induces a glycolytic switch in pulmonary arterial endothelial cells via the mitochondrial translocation of endothelial nitric oxide synthase

Xutong Sun, Sanjiv Kumar, Shruti Sharma, Saurabh Aggarwal, Qing Lu, Christine Gross, Olga Rafikova, Sung Gon Lee, Sridevi Dasarathy, Yali Hou, Mary Louise Meadows, Weihong Han, Yunchao Su, Jeffrey R. Fineman, Stephen Matthew Black

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Recent studies have indicated that, during the development of pulmonary hypertension (PH), there is a switch from oxidative phosphorylation to glycolysis in the pulmonary endothelium. However, the mechanisms underlying this phenomenon have not been elucidated. Endothelin (ET)-1, an endothelial-derived vasoconstrictor peptide, is increased in PH, and has been shown to play an important role in the oxidative stress associated with PH. Thus, in this study, we investigated whether there was a potential link between increases in ET-1 andmitochondrial remodeling. Our data indicate that ET-1 induces the redistribution of endothelial nitric oxide synthase (eNOS) from the plasma membrane to the mitochondria in pulmonary arterial endothelial cells, and that this was dependent on eNOS uncoupling. We also found that ET-1 disturbed carnitine metabolism, resulting in the attenuation of mitochondrial bioenergetics. However, ATP levels were unchanged due to a compensatory increase in glycolysis. Further mechanistic investigations demonstrated that ET-1 mediated the redistribution of eNOS via the phosphorylation of eNOS at Thr495 by protein kinase C δ. In addition, the glycolytic switch appeared to be dependent on mitochondrial-derived reactive oxygen species that led to the activation of hypoxia-inducible factor signaling. Finally, the cell culture data were con firmed in vivo using the monocrotaline rat model of PH. Thus, we conclude that ET-1 induces a glycolytic switch in pulmonary arterial endothelial cells via the redistribution of uncoupled eNOS to the mitochondria, and that preventing this eventmay be anapproachfor the treatment of PH.

Original languageEnglish (US)
Pages (from-to)1084-1095
Number of pages12
JournalAmerican journal of respiratory cell and molecular biology
Volume50
Issue number6
DOIs
StatePublished - Jan 1 2014

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Nitric Oxide Synthase Type III
Endothelial cells
Endothelin-1
Pulmonary Hypertension
Endothelial Cells
Switches
Lung
Mitochondria
Glycolysis
Monocrotaline
Phosphorylation
Oxidative stress
Carnitine
Oxidative Phosphorylation
Vasoconstrictor Agents
Cell membranes
Cell culture
Metabolism
Protein Kinase C
Energy Metabolism

Keywords

  • Endothelial nitric oxide synthase uncoupling
  • Mitochondrial bioenergetics
  • Peroxynitrite
  • Protein kinase C δ
  • Superoxide

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Endothelin-1 induces a glycolytic switch in pulmonary arterial endothelial cells via the mitochondrial translocation of endothelial nitric oxide synthase. / Sun, Xutong; Kumar, Sanjiv; Sharma, Shruti; Aggarwal, Saurabh; Lu, Qing; Gross, Christine; Rafikova, Olga; Lee, Sung Gon; Dasarathy, Sridevi; Hou, Yali; Meadows, Mary Louise; Han, Weihong; Su, Yunchao; Fineman, Jeffrey R.; Black, Stephen Matthew.

In: American journal of respiratory cell and molecular biology, Vol. 50, No. 6, 01.01.2014, p. 1084-1095.

Research output: Contribution to journalArticle

Sun, Xutong ; Kumar, Sanjiv ; Sharma, Shruti ; Aggarwal, Saurabh ; Lu, Qing ; Gross, Christine ; Rafikova, Olga ; Lee, Sung Gon ; Dasarathy, Sridevi ; Hou, Yali ; Meadows, Mary Louise ; Han, Weihong ; Su, Yunchao ; Fineman, Jeffrey R. ; Black, Stephen Matthew. / Endothelin-1 induces a glycolytic switch in pulmonary arterial endothelial cells via the mitochondrial translocation of endothelial nitric oxide synthase. In: American journal of respiratory cell and molecular biology. 2014 ; Vol. 50, No. 6. pp. 1084-1095.
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AU - Sun, Xutong

AU - Kumar, Sanjiv

AU - Sharma, Shruti

AU - Aggarwal, Saurabh

AU - Lu, Qing

AU - Gross, Christine

AU - Rafikova, Olga

AU - Lee, Sung Gon

AU - Dasarathy, Sridevi

AU - Hou, Yali

AU - Meadows, Mary Louise

AU - Han, Weihong

AU - Su, Yunchao

AU - Fineman, Jeffrey R.

AU - Black, Stephen Matthew

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Y1 - 2014/1/1

N2 - Recent studies have indicated that, during the development of pulmonary hypertension (PH), there is a switch from oxidative phosphorylation to glycolysis in the pulmonary endothelium. However, the mechanisms underlying this phenomenon have not been elucidated. Endothelin (ET)-1, an endothelial-derived vasoconstrictor peptide, is increased in PH, and has been shown to play an important role in the oxidative stress associated with PH. Thus, in this study, we investigated whether there was a potential link between increases in ET-1 andmitochondrial remodeling. Our data indicate that ET-1 induces the redistribution of endothelial nitric oxide synthase (eNOS) from the plasma membrane to the mitochondria in pulmonary arterial endothelial cells, and that this was dependent on eNOS uncoupling. We also found that ET-1 disturbed carnitine metabolism, resulting in the attenuation of mitochondrial bioenergetics. However, ATP levels were unchanged due to a compensatory increase in glycolysis. Further mechanistic investigations demonstrated that ET-1 mediated the redistribution of eNOS via the phosphorylation of eNOS at Thr495 by protein kinase C δ. In addition, the glycolytic switch appeared to be dependent on mitochondrial-derived reactive oxygen species that led to the activation of hypoxia-inducible factor signaling. Finally, the cell culture data were con firmed in vivo using the monocrotaline rat model of PH. Thus, we conclude that ET-1 induces a glycolytic switch in pulmonary arterial endothelial cells via the redistribution of uncoupled eNOS to the mitochondria, and that preventing this eventmay be anapproachfor the treatment of PH.

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