Endothelin-1 is not involved in serotonin-induced coronary spasm in a swine model

Tohru Fukai, Kensuke Egashira, Kohtaro Numaguchi, Hiroshi Hata, Teisuke Takahashi, Hiromitsu Kasuya, Makoto Sakata, Hiroaki Shimokawa, Akira Takeshita

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: The role of endothelin-1 (ET-1) in the pathogenesis of coronary artery spasm is not well understood. We aimed to determine if ET-1 is involved in serotonin-induced coronary spasm in the swine model. Methods: In 10 miniature pigs, a segment of the left anterior descending coronary artery was denuded and irradiated with X-ray. Three months after endothelial denudation, coronary vasomotion was assessed in vivo by quantitative arteriography. Results: Intracoronary serotonin at 10 μg/kg provoked coronary spasm (augmented narrowing of the luminal diameter) at the denuded site (diameter reduction 93 ± 4%) but not at the non-denuded control site (19 ± 4%, P < 0.01) associated with ST segment elevation in the region perfused by the denuded artery. Intracoronary administration of ET-1 at 25 ng/kg caused mild vasoconstriction of the denuded (26 ± 4) and non-denuded site (16 ± 3%, n.s.), but provoked ST segment elevation in the regions perfused by both the denuded and non-denuded arteries. The treatment with an endothelin antagonist (BQ123 0.1 mg/kg) significantly attenuated coronary vasoconstriction and ST segment elevation evoked with ET-1, but did not alter serotonin-induced vasoconstriction either at the denuded or control site. Conclusions: The results of this study suggest that endogenous ET-1 may not be involved in the pathogenesis of serotonin-induced coronary spasm in our swine model.

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalCardiovascular Research
Volume30
Issue number2
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Spasm
Endothelin-1
Serotonin
Swine
Vasoconstriction
Coronary Vessels
Arteries
Angiography
X-Rays

Keywords

  • Coronary artery tone
  • Endothelin antagonist
  • Endothelin-1
  • Pig, coronary artery
  • Serotonin

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Fukai, T., Egashira, K., Numaguchi, K., Hata, H., Takahashi, T., Kasuya, H., ... Takeshita, A. (1995). Endothelin-1 is not involved in serotonin-induced coronary spasm in a swine model. Cardiovascular Research, 30(2), 193-199. https://doi.org/10.1016/S0008-6363(95)00022-4

Endothelin-1 is not involved in serotonin-induced coronary spasm in a swine model. / Fukai, Tohru; Egashira, Kensuke; Numaguchi, Kohtaro; Hata, Hiroshi; Takahashi, Teisuke; Kasuya, Hiromitsu; Sakata, Makoto; Shimokawa, Hiroaki; Takeshita, Akira.

In: Cardiovascular Research, Vol. 30, No. 2, 01.01.1995, p. 193-199.

Research output: Contribution to journalArticle

Fukai, T, Egashira, K, Numaguchi, K, Hata, H, Takahashi, T, Kasuya, H, Sakata, M, Shimokawa, H & Takeshita, A 1995, 'Endothelin-1 is not involved in serotonin-induced coronary spasm in a swine model', Cardiovascular Research, vol. 30, no. 2, pp. 193-199. https://doi.org/10.1016/S0008-6363(95)00022-4
Fukai, Tohru ; Egashira, Kensuke ; Numaguchi, Kohtaro ; Hata, Hiroshi ; Takahashi, Teisuke ; Kasuya, Hiromitsu ; Sakata, Makoto ; Shimokawa, Hiroaki ; Takeshita, Akira. / Endothelin-1 is not involved in serotonin-induced coronary spasm in a swine model. In: Cardiovascular Research. 1995 ; Vol. 30, No. 2. pp. 193-199.
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abstract = "Objectives: The role of endothelin-1 (ET-1) in the pathogenesis of coronary artery spasm is not well understood. We aimed to determine if ET-1 is involved in serotonin-induced coronary spasm in the swine model. Methods: In 10 miniature pigs, a segment of the left anterior descending coronary artery was denuded and irradiated with X-ray. Three months after endothelial denudation, coronary vasomotion was assessed in vivo by quantitative arteriography. Results: Intracoronary serotonin at 10 μg/kg provoked coronary spasm (augmented narrowing of the luminal diameter) at the denuded site (diameter reduction 93 ± 4{\%}) but not at the non-denuded control site (19 ± 4{\%}, P < 0.01) associated with ST segment elevation in the region perfused by the denuded artery. Intracoronary administration of ET-1 at 25 ng/kg caused mild vasoconstriction of the denuded (26 ± 4) and non-denuded site (16 ± 3{\%}, n.s.), but provoked ST segment elevation in the regions perfused by both the denuded and non-denuded arteries. The treatment with an endothelin antagonist (BQ123 0.1 mg/kg) significantly attenuated coronary vasoconstriction and ST segment elevation evoked with ET-1, but did not alter serotonin-induced vasoconstriction either at the denuded or control site. Conclusions: The results of this study suggest that endogenous ET-1 may not be involved in the pathogenesis of serotonin-induced coronary spasm in our swine model.",
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AU - Hata, Hiroshi

AU - Takahashi, Teisuke

AU - Kasuya, Hiromitsu

AU - Sakata, Makoto

AU - Shimokawa, Hiroaki

AU - Takeshita, Akira

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N2 - Objectives: The role of endothelin-1 (ET-1) in the pathogenesis of coronary artery spasm is not well understood. We aimed to determine if ET-1 is involved in serotonin-induced coronary spasm in the swine model. Methods: In 10 miniature pigs, a segment of the left anterior descending coronary artery was denuded and irradiated with X-ray. Three months after endothelial denudation, coronary vasomotion was assessed in vivo by quantitative arteriography. Results: Intracoronary serotonin at 10 μg/kg provoked coronary spasm (augmented narrowing of the luminal diameter) at the denuded site (diameter reduction 93 ± 4%) but not at the non-denuded control site (19 ± 4%, P < 0.01) associated with ST segment elevation in the region perfused by the denuded artery. Intracoronary administration of ET-1 at 25 ng/kg caused mild vasoconstriction of the denuded (26 ± 4) and non-denuded site (16 ± 3%, n.s.), but provoked ST segment elevation in the regions perfused by both the denuded and non-denuded arteries. The treatment with an endothelin antagonist (BQ123 0.1 mg/kg) significantly attenuated coronary vasoconstriction and ST segment elevation evoked with ET-1, but did not alter serotonin-induced vasoconstriction either at the denuded or control site. Conclusions: The results of this study suggest that endogenous ET-1 may not be involved in the pathogenesis of serotonin-induced coronary spasm in our swine model.

AB - Objectives: The role of endothelin-1 (ET-1) in the pathogenesis of coronary artery spasm is not well understood. We aimed to determine if ET-1 is involved in serotonin-induced coronary spasm in the swine model. Methods: In 10 miniature pigs, a segment of the left anterior descending coronary artery was denuded and irradiated with X-ray. Three months after endothelial denudation, coronary vasomotion was assessed in vivo by quantitative arteriography. Results: Intracoronary serotonin at 10 μg/kg provoked coronary spasm (augmented narrowing of the luminal diameter) at the denuded site (diameter reduction 93 ± 4%) but not at the non-denuded control site (19 ± 4%, P < 0.01) associated with ST segment elevation in the region perfused by the denuded artery. Intracoronary administration of ET-1 at 25 ng/kg caused mild vasoconstriction of the denuded (26 ± 4) and non-denuded site (16 ± 3%, n.s.), but provoked ST segment elevation in the regions perfused by both the denuded and non-denuded arteries. The treatment with an endothelin antagonist (BQ123 0.1 mg/kg) significantly attenuated coronary vasoconstriction and ST segment elevation evoked with ET-1, but did not alter serotonin-induced vasoconstriction either at the denuded or control site. Conclusions: The results of this study suggest that endogenous ET-1 may not be involved in the pathogenesis of serotonin-induced coronary spasm in our swine model.

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