Endothelin-1 Stimulates Leptin Production in Adipocytes

Yumei Xiong, Hirokazu Tanaka, James A. Richardson, S. Clay Williams, Clive A. Slaughter, Motonao Nakamura, Jin Long Chen, Masashi Yanagisawa

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Leptin is an adipocyte-derived hormone that regulates body fat stores and feeding behavior. In an effort to identify endogenous diffusible modulators of leptin production, we found that endothelin-1 (ET-1) up-regulates leptin expression in adipocytes. ET-1 is as potent and efficacious as insulin in stimulating leptin production in two different adipocyte cell lines. Endothelins stimulate leptin production via the endothelin-A receptor (ET A ), as judged by a potency rank order of ET-1 ≫ ET-3. We detected expression of ET A but not ET B in both cell lines by Northern blot analysis. In addition, the ET A -selective antagonist FR139317 inhibited ET-1-induced leptin expression more potently than did the ET B -selective antagonist BQ788. ET-1 and insulin positively interact with each other in increasing leptin production in adipocytes. In primary mouse white fat cells, we detected expression of both ET A and ET B by Northern blot and in situ hybridization analyses. We conclude that ET-1 stimulates leptin production via the ET A receptor in cultured adipocytes.

Original languageEnglish (US)
Pages (from-to)28471-28477
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number30
DOIs
StatePublished - Jul 27 2001
Externally publishedYes

Fingerprint

Endothelin-1
Leptin
Adipocytes
Northern Blotting
White Adipocytes
Fats
Cells
Insulin
Endothelin A Receptors
Cell Line
Endothelins
Feeding Behavior
Modulators
In Situ Hybridization
Adipose Tissue
Up-Regulation
Hormones

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Xiong, Y., Tanaka, H., Richardson, J. A., Williams, S. C., Slaughter, C. A., Nakamura, M., ... Yanagisawa, M. (2001). Endothelin-1 Stimulates Leptin Production in Adipocytes. Journal of Biological Chemistry, 276(30), 28471-28477. https://doi.org/10.1074/jbc.M103478200

Endothelin-1 Stimulates Leptin Production in Adipocytes. / Xiong, Yumei; Tanaka, Hirokazu; Richardson, James A.; Williams, S. Clay; Slaughter, Clive A.; Nakamura, Motonao; Chen, Jin Long; Yanagisawa, Masashi.

In: Journal of Biological Chemistry, Vol. 276, No. 30, 27.07.2001, p. 28471-28477.

Research output: Contribution to journalArticle

Xiong, Y, Tanaka, H, Richardson, JA, Williams, SC, Slaughter, CA, Nakamura, M, Chen, JL & Yanagisawa, M 2001, 'Endothelin-1 Stimulates Leptin Production in Adipocytes', Journal of Biological Chemistry, vol. 276, no. 30, pp. 28471-28477. https://doi.org/10.1074/jbc.M103478200
Xiong Y, Tanaka H, Richardson JA, Williams SC, Slaughter CA, Nakamura M et al. Endothelin-1 Stimulates Leptin Production in Adipocytes. Journal of Biological Chemistry. 2001 Jul 27;276(30):28471-28477. https://doi.org/10.1074/jbc.M103478200
Xiong, Yumei ; Tanaka, Hirokazu ; Richardson, James A. ; Williams, S. Clay ; Slaughter, Clive A. ; Nakamura, Motonao ; Chen, Jin Long ; Yanagisawa, Masashi. / Endothelin-1 Stimulates Leptin Production in Adipocytes. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 30. pp. 28471-28477.
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