Endotoxin adherence to and elution from two casting alloys

Kent I. Knovrnschild, Carol A Lefebvre, George S. Schuster, Use M. Payant, Francois M. Gagnon

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The purpose of this study was to compare tbe relative affinity of bacterial lipopolysaccbaride for two casting alloys with varied surface finisbes by measuring lipopolysaccharide adberence and elution. Air borne particle abraded and polisbed Rexillium III and Harmony Hard alloy discs were exposed to 600,000 endotoxin unlts/mL E. coli lipopolysaccbaride in water for 24 bours, whereas control discs were placed in 37°C, lipopolysaccharidefree water. Lipopolysaccbarlde-exposed and control discs were tben transferred every 24 hours to fresh, lipopolysaccharide-free water for up to 96 hours, and previously incubated eluates were tested for the presence of lipopolysaccbaride. Initial lipopolysaccbaride adherence to abraded Rexillium III discs was significantly greater than lipopolysaccharide adherence to the other groups (P < .05). Polished and abraded Rexillium III also exhibited significantly greater lipopolysaccharide elution (P = .0001. However, after 96 hours, more than 99% of the initially adbering lipopolysaccharide remained on both Rexillium 111 and Harmony Hard discs, regardless of surface treatment.

Original languageEnglish (US)
Pages (from-to)22-29
Number of pages8
JournalInternational Journal of Prosthodontics
Volume7
Issue number1
StatePublished - Jan 1 1994

Fingerprint

Endotoxins
Lipopolysaccharides
Water
Air
Escherichia coli
Rexillium III
Harmony Hard

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Knovrnschild, K. I., Lefebvre, C. A., Schuster, G. S., Payant, U. M., & Gagnon, F. M. (1994). Endotoxin adherence to and elution from two casting alloys. International Journal of Prosthodontics, 7(1), 22-29.

Endotoxin adherence to and elution from two casting alloys. / Knovrnschild, Kent I.; Lefebvre, Carol A; Schuster, George S.; Payant, Use M.; Gagnon, Francois M.

In: International Journal of Prosthodontics, Vol. 7, No. 1, 01.01.1994, p. 22-29.

Research output: Contribution to journalArticle

Knovrnschild, KI, Lefebvre, CA, Schuster, GS, Payant, UM & Gagnon, FM 1994, 'Endotoxin adherence to and elution from two casting alloys', International Journal of Prosthodontics, vol. 7, no. 1, pp. 22-29.
Knovrnschild KI, Lefebvre CA, Schuster GS, Payant UM, Gagnon FM. Endotoxin adherence to and elution from two casting alloys. International Journal of Prosthodontics. 1994 Jan 1;7(1):22-29.
Knovrnschild, Kent I. ; Lefebvre, Carol A ; Schuster, George S. ; Payant, Use M. ; Gagnon, Francois M. / Endotoxin adherence to and elution from two casting alloys. In: International Journal of Prosthodontics. 1994 ; Vol. 7, No. 1. pp. 22-29.
@article{39101b7617de491895e7d439f8d5dc2e,
title = "Endotoxin adherence to and elution from two casting alloys",
abstract = "The purpose of this study was to compare tbe relative affinity of bacterial lipopolysaccbaride for two casting alloys with varied surface finisbes by measuring lipopolysaccharide adberence and elution. Air borne particle abraded and polisbed Rexillium III and Harmony Hard alloy discs were exposed to 600,000 endotoxin unlts/mL E. coli lipopolysaccbaride in water for 24 bours, whereas control discs were placed in 37°C, lipopolysaccharidefree water. Lipopolysaccbarlde-exposed and control discs were tben transferred every 24 hours to fresh, lipopolysaccharide-free water for up to 96 hours, and previously incubated eluates were tested for the presence of lipopolysaccbaride. Initial lipopolysaccbaride adherence to abraded Rexillium III discs was significantly greater than lipopolysaccharide adherence to the other groups (P < .05). Polished and abraded Rexillium III also exhibited significantly greater lipopolysaccharide elution (P = .0001. However, after 96 hours, more than 99{\%} of the initially adbering lipopolysaccharide remained on both Rexillium 111 and Harmony Hard discs, regardless of surface treatment.",
author = "Knovrnschild, {Kent I.} and Lefebvre, {Carol A} and Schuster, {George S.} and Payant, {Use M.} and Gagnon, {Francois M.}",
year = "1994",
month = "1",
day = "1",
language = "English (US)",
volume = "7",
pages = "22--29",
journal = "International Journal of Prosthodontics",
issn = "0893-2174",
publisher = "Quintessence Publishing Company",
number = "1",

}

TY - JOUR

T1 - Endotoxin adherence to and elution from two casting alloys

AU - Knovrnschild, Kent I.

AU - Lefebvre, Carol A

AU - Schuster, George S.

AU - Payant, Use M.

AU - Gagnon, Francois M.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The purpose of this study was to compare tbe relative affinity of bacterial lipopolysaccbaride for two casting alloys with varied surface finisbes by measuring lipopolysaccharide adberence and elution. Air borne particle abraded and polisbed Rexillium III and Harmony Hard alloy discs were exposed to 600,000 endotoxin unlts/mL E. coli lipopolysaccbaride in water for 24 bours, whereas control discs were placed in 37°C, lipopolysaccharidefree water. Lipopolysaccbarlde-exposed and control discs were tben transferred every 24 hours to fresh, lipopolysaccharide-free water for up to 96 hours, and previously incubated eluates were tested for the presence of lipopolysaccbaride. Initial lipopolysaccbaride adherence to abraded Rexillium III discs was significantly greater than lipopolysaccharide adherence to the other groups (P < .05). Polished and abraded Rexillium III also exhibited significantly greater lipopolysaccharide elution (P = .0001. However, after 96 hours, more than 99% of the initially adbering lipopolysaccharide remained on both Rexillium 111 and Harmony Hard discs, regardless of surface treatment.

AB - The purpose of this study was to compare tbe relative affinity of bacterial lipopolysaccbaride for two casting alloys with varied surface finisbes by measuring lipopolysaccharide adberence and elution. Air borne particle abraded and polisbed Rexillium III and Harmony Hard alloy discs were exposed to 600,000 endotoxin unlts/mL E. coli lipopolysaccbaride in water for 24 bours, whereas control discs were placed in 37°C, lipopolysaccharidefree water. Lipopolysaccbarlde-exposed and control discs were tben transferred every 24 hours to fresh, lipopolysaccharide-free water for up to 96 hours, and previously incubated eluates were tested for the presence of lipopolysaccbaride. Initial lipopolysaccbaride adherence to abraded Rexillium III discs was significantly greater than lipopolysaccharide adherence to the other groups (P < .05). Polished and abraded Rexillium III also exhibited significantly greater lipopolysaccharide elution (P = .0001. However, after 96 hours, more than 99% of the initially adbering lipopolysaccharide remained on both Rexillium 111 and Harmony Hard discs, regardless of surface treatment.

UR - http://www.scopus.com/inward/record.url?scp=0028318002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028318002&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 22

EP - 29

JO - International Journal of Prosthodontics

JF - International Journal of Prosthodontics

SN - 0893-2174

IS - 1

ER -