Endotoxin and cisplatin synergistically stimulate TNF-α production by renal epithelial cells

Ganesan Ramesh, Scot R. Kimball, Leonard S. Jefferson, W. Brian Reeves

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Acute renal failure often occurs in the clinical setting of multiple renal insults. Tumor necrosis factor-α (TNF-α) has been implicated in the pathogenesis of cisplatin nephrotoxicity, ischemia-reperfusion injury, and endotoxin-induced acute renal failure. The current studies examined the interactions between cisplatin and endotoxin with particular emphasis on TNF-α production. Treatment of cultured murine proximal tubule cells (TKPTS cells) with cisplatin resulted in a modest production of TNF-α, while treatment with endotoxin did not result in any TNF-α production. However, the combination of cisplatin and endotoxin resulted in large amounts of TNF-α synthesis and secretion. The stimulation of TNF-α production was dependent on cisplatin-induced activation of p38 MAPK and was associated with phosphorylation of the translation initiation factor eIF4E and its upstream kinase Mnk1. Inhibition of p38 MAPK and, to a lesser extent, ERK, reduced cisplatin+endotoxin-stimulated TNF-α production and phosphorylation of Mnk1 and eIF4E. Synergy between cisplatin and endotoxin was also observed in certain tumor cell lines, but not in macrophages. In macrophages, in contrast to TKPTS cells, endotoxin alone activated p38 MAPK and stimulated TNF-α production with no added impact by cisplatin. The combination of cisplatin and endotoxin did not result in synergistic production of other cytokines, e.g., MCP-1 and MIP2, by TKPTS cells. In summary, these studies indicate that cisplatin sensitizes renal epithelial cells to endotoxin and dramatically increases the translation of TNF-α mRNA in a p38 MAPK-dependent manner. These interactions between cisplatin and endotoxin may be relevant to the pathogenesis of cisplatin nephrotoxicity in humans.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume292
Issue number2
DOIs
StatePublished - Feb 1 2007

Fingerprint

Endotoxins
Cisplatin
Tumor Necrosis Factor-alpha
Epithelial Cells
Kidney
p38 Mitogen-Activated Protein Kinases
Acute Kidney Injury
Macrophages
Phosphorylation
Peptide Initiation Factors
Tumor Cell Line
Reperfusion Injury
Phosphotransferases
Cytokines
Messenger RNA

Keywords

  • Acute renal failure
  • eIF4E
  • LPS
  • Mnk1
  • p38 MAPK

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Endotoxin and cisplatin synergistically stimulate TNF-α production by renal epithelial cells. / Ramesh, Ganesan; Kimball, Scot R.; Jefferson, Leonard S.; Reeves, W. Brian.

In: American Journal of Physiology - Renal Physiology, Vol. 292, No. 2, 01.02.2007.

Research output: Contribution to journalArticle

Ramesh, Ganesan ; Kimball, Scot R. ; Jefferson, Leonard S. ; Reeves, W. Brian. / Endotoxin and cisplatin synergistically stimulate TNF-α production by renal epithelial cells. In: American Journal of Physiology - Renal Physiology. 2007 ; Vol. 292, No. 2.
@article{87f4007612cf4b2c9f905c23e9eeee97,
title = "Endotoxin and cisplatin synergistically stimulate TNF-α production by renal epithelial cells",
abstract = "Acute renal failure often occurs in the clinical setting of multiple renal insults. Tumor necrosis factor-α (TNF-α) has been implicated in the pathogenesis of cisplatin nephrotoxicity, ischemia-reperfusion injury, and endotoxin-induced acute renal failure. The current studies examined the interactions between cisplatin and endotoxin with particular emphasis on TNF-α production. Treatment of cultured murine proximal tubule cells (TKPTS cells) with cisplatin resulted in a modest production of TNF-α, while treatment with endotoxin did not result in any TNF-α production. However, the combination of cisplatin and endotoxin resulted in large amounts of TNF-α synthesis and secretion. The stimulation of TNF-α production was dependent on cisplatin-induced activation of p38 MAPK and was associated with phosphorylation of the translation initiation factor eIF4E and its upstream kinase Mnk1. Inhibition of p38 MAPK and, to a lesser extent, ERK, reduced cisplatin+endotoxin-stimulated TNF-α production and phosphorylation of Mnk1 and eIF4E. Synergy between cisplatin and endotoxin was also observed in certain tumor cell lines, but not in macrophages. In macrophages, in contrast to TKPTS cells, endotoxin alone activated p38 MAPK and stimulated TNF-α production with no added impact by cisplatin. The combination of cisplatin and endotoxin did not result in synergistic production of other cytokines, e.g., MCP-1 and MIP2, by TKPTS cells. In summary, these studies indicate that cisplatin sensitizes renal epithelial cells to endotoxin and dramatically increases the translation of TNF-α mRNA in a p38 MAPK-dependent manner. These interactions between cisplatin and endotoxin may be relevant to the pathogenesis of cisplatin nephrotoxicity in humans.",
keywords = "Acute renal failure, eIF4E, LPS, Mnk1, p38 MAPK",
author = "Ganesan Ramesh and Kimball, {Scot R.} and Jefferson, {Leonard S.} and Reeves, {W. Brian}",
year = "2007",
month = "2",
day = "1",
doi = "10.1152/ajprenal.00277.2006",
language = "English (US)",
volume = "292",
journal = "American Journal of Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Endotoxin and cisplatin synergistically stimulate TNF-α production by renal epithelial cells

AU - Ramesh, Ganesan

AU - Kimball, Scot R.

AU - Jefferson, Leonard S.

AU - Reeves, W. Brian

PY - 2007/2/1

Y1 - 2007/2/1

N2 - Acute renal failure often occurs in the clinical setting of multiple renal insults. Tumor necrosis factor-α (TNF-α) has been implicated in the pathogenesis of cisplatin nephrotoxicity, ischemia-reperfusion injury, and endotoxin-induced acute renal failure. The current studies examined the interactions between cisplatin and endotoxin with particular emphasis on TNF-α production. Treatment of cultured murine proximal tubule cells (TKPTS cells) with cisplatin resulted in a modest production of TNF-α, while treatment with endotoxin did not result in any TNF-α production. However, the combination of cisplatin and endotoxin resulted in large amounts of TNF-α synthesis and secretion. The stimulation of TNF-α production was dependent on cisplatin-induced activation of p38 MAPK and was associated with phosphorylation of the translation initiation factor eIF4E and its upstream kinase Mnk1. Inhibition of p38 MAPK and, to a lesser extent, ERK, reduced cisplatin+endotoxin-stimulated TNF-α production and phosphorylation of Mnk1 and eIF4E. Synergy between cisplatin and endotoxin was also observed in certain tumor cell lines, but not in macrophages. In macrophages, in contrast to TKPTS cells, endotoxin alone activated p38 MAPK and stimulated TNF-α production with no added impact by cisplatin. The combination of cisplatin and endotoxin did not result in synergistic production of other cytokines, e.g., MCP-1 and MIP2, by TKPTS cells. In summary, these studies indicate that cisplatin sensitizes renal epithelial cells to endotoxin and dramatically increases the translation of TNF-α mRNA in a p38 MAPK-dependent manner. These interactions between cisplatin and endotoxin may be relevant to the pathogenesis of cisplatin nephrotoxicity in humans.

AB - Acute renal failure often occurs in the clinical setting of multiple renal insults. Tumor necrosis factor-α (TNF-α) has been implicated in the pathogenesis of cisplatin nephrotoxicity, ischemia-reperfusion injury, and endotoxin-induced acute renal failure. The current studies examined the interactions between cisplatin and endotoxin with particular emphasis on TNF-α production. Treatment of cultured murine proximal tubule cells (TKPTS cells) with cisplatin resulted in a modest production of TNF-α, while treatment with endotoxin did not result in any TNF-α production. However, the combination of cisplatin and endotoxin resulted in large amounts of TNF-α synthesis and secretion. The stimulation of TNF-α production was dependent on cisplatin-induced activation of p38 MAPK and was associated with phosphorylation of the translation initiation factor eIF4E and its upstream kinase Mnk1. Inhibition of p38 MAPK and, to a lesser extent, ERK, reduced cisplatin+endotoxin-stimulated TNF-α production and phosphorylation of Mnk1 and eIF4E. Synergy between cisplatin and endotoxin was also observed in certain tumor cell lines, but not in macrophages. In macrophages, in contrast to TKPTS cells, endotoxin alone activated p38 MAPK and stimulated TNF-α production with no added impact by cisplatin. The combination of cisplatin and endotoxin did not result in synergistic production of other cytokines, e.g., MCP-1 and MIP2, by TKPTS cells. In summary, these studies indicate that cisplatin sensitizes renal epithelial cells to endotoxin and dramatically increases the translation of TNF-α mRNA in a p38 MAPK-dependent manner. These interactions between cisplatin and endotoxin may be relevant to the pathogenesis of cisplatin nephrotoxicity in humans.

KW - Acute renal failure

KW - eIF4E

KW - LPS

KW - Mnk1

KW - p38 MAPK

UR - http://www.scopus.com/inward/record.url?scp=33846889997&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846889997&partnerID=8YFLogxK

U2 - 10.1152/ajprenal.00277.2006

DO - 10.1152/ajprenal.00277.2006

M3 - Article

VL - 292

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 1931-857X

IS - 2

ER -