Energy expenditure and body composition of chronically maintained decerebrate rats in the fed and fasted condition

Ruth Babette Harris, Emily W. Kelso, William P. Flatt, Timothy J. Bartness, Harvey J. Grill

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The contribution of the caudal brainstem to adaptation to starvation was tested using chronically maintained decerebrate (CD) and neurologically intact controls. All rats were gavage fed an amount of diet that maintained weight gain in controls. CD rats were subjected to a two-stage surgery to produce a complete transection of the neuroaxis at the mesodiencephalic juncture.One week later, the rats were housed in an indirect calorimeter, and 24 h energy expenditure was measured for 4 d.One half of each of the CD and control groups was then starved for 48 h. Fed CD rats maintained a lower body temperature (35 C), a similar energy expenditure per unit fat-free mass but an elevated respiratory quotient compared with controls. They gained less weight, had 20% less lean tissue, and had 60% more fat than controls. Circulating leptin, adiponectin, and insulin were elevated, glucose was normal, but testosterone was dramatically reduced. Responses to starvation were similar in CD and controls; they reduced energy expenditure, decreased respiratory quotient, indicating lipid utilization, defended body temperature, mobilized fat, decreased serum leptin and insulin, and regulated plasma glucose. These data clearly demonstrate that the isolated caudal brainstem is sufficient to mediate many aspects of the energetic response to starvation. In intact animals, these responses may be refined by a contribution by more rostral brain areas or by communication between fore- and hind-brain. In the absence of communication from the fore-brain, the caudal brainstem is inadequate for maintenance of testosterone levels or lean tissue in fed or fasted animals.

Original languageEnglish (US)
Pages (from-to)1365-1376
Number of pages12
JournalEndocrinology
Volume147
Issue number3
DOIs
StatePublished - Mar 1 2006

Fingerprint

Body Composition
Energy Metabolism
Starvation
Brain Stem
Fats
Leptin
Body Temperature
Testosterone
Brain
Communication
Insulin
Glucose
Rhombencephalon
Adiponectin
Weight Gain
Maintenance
Diet
Lipids
Weights and Measures
Control Groups

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Energy expenditure and body composition of chronically maintained decerebrate rats in the fed and fasted condition. / Harris, Ruth Babette; Kelso, Emily W.; Flatt, William P.; Bartness, Timothy J.; Grill, Harvey J.

In: Endocrinology, Vol. 147, No. 3, 01.03.2006, p. 1365-1376.

Research output: Contribution to journalArticle

Harris, Ruth Babette ; Kelso, Emily W. ; Flatt, William P. ; Bartness, Timothy J. ; Grill, Harvey J. / Energy expenditure and body composition of chronically maintained decerebrate rats in the fed and fasted condition. In: Endocrinology. 2006 ; Vol. 147, No. 3. pp. 1365-1376.
@article{9451e8e0b91a4c2fbc4a066d90260551,
title = "Energy expenditure and body composition of chronically maintained decerebrate rats in the fed and fasted condition",
abstract = "The contribution of the caudal brainstem to adaptation to starvation was tested using chronically maintained decerebrate (CD) and neurologically intact controls. All rats were gavage fed an amount of diet that maintained weight gain in controls. CD rats were subjected to a two-stage surgery to produce a complete transection of the neuroaxis at the mesodiencephalic juncture.One week later, the rats were housed in an indirect calorimeter, and 24 h energy expenditure was measured for 4 d.One half of each of the CD and control groups was then starved for 48 h. Fed CD rats maintained a lower body temperature (35 C), a similar energy expenditure per unit fat-free mass but an elevated respiratory quotient compared with controls. They gained less weight, had 20{\%} less lean tissue, and had 60{\%} more fat than controls. Circulating leptin, adiponectin, and insulin were elevated, glucose was normal, but testosterone was dramatically reduced. Responses to starvation were similar in CD and controls; they reduced energy expenditure, decreased respiratory quotient, indicating lipid utilization, defended body temperature, mobilized fat, decreased serum leptin and insulin, and regulated plasma glucose. These data clearly demonstrate that the isolated caudal brainstem is sufficient to mediate many aspects of the energetic response to starvation. In intact animals, these responses may be refined by a contribution by more rostral brain areas or by communication between fore- and hind-brain. In the absence of communication from the fore-brain, the caudal brainstem is inadequate for maintenance of testosterone levels or lean tissue in fed or fasted animals.",
author = "Harris, {Ruth Babette} and Kelso, {Emily W.} and Flatt, {William P.} and Bartness, {Timothy J.} and Grill, {Harvey J.}",
year = "2006",
month = "3",
day = "1",
doi = "10.1210/en.2005-1156",
language = "English (US)",
volume = "147",
pages = "1365--1376",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - Energy expenditure and body composition of chronically maintained decerebrate rats in the fed and fasted condition

AU - Harris, Ruth Babette

AU - Kelso, Emily W.

AU - Flatt, William P.

AU - Bartness, Timothy J.

AU - Grill, Harvey J.

PY - 2006/3/1

Y1 - 2006/3/1

N2 - The contribution of the caudal brainstem to adaptation to starvation was tested using chronically maintained decerebrate (CD) and neurologically intact controls. All rats were gavage fed an amount of diet that maintained weight gain in controls. CD rats were subjected to a two-stage surgery to produce a complete transection of the neuroaxis at the mesodiencephalic juncture.One week later, the rats were housed in an indirect calorimeter, and 24 h energy expenditure was measured for 4 d.One half of each of the CD and control groups was then starved for 48 h. Fed CD rats maintained a lower body temperature (35 C), a similar energy expenditure per unit fat-free mass but an elevated respiratory quotient compared with controls. They gained less weight, had 20% less lean tissue, and had 60% more fat than controls. Circulating leptin, adiponectin, and insulin were elevated, glucose was normal, but testosterone was dramatically reduced. Responses to starvation were similar in CD and controls; they reduced energy expenditure, decreased respiratory quotient, indicating lipid utilization, defended body temperature, mobilized fat, decreased serum leptin and insulin, and regulated plasma glucose. These data clearly demonstrate that the isolated caudal brainstem is sufficient to mediate many aspects of the energetic response to starvation. In intact animals, these responses may be refined by a contribution by more rostral brain areas or by communication between fore- and hind-brain. In the absence of communication from the fore-brain, the caudal brainstem is inadequate for maintenance of testosterone levels or lean tissue in fed or fasted animals.

AB - The contribution of the caudal brainstem to adaptation to starvation was tested using chronically maintained decerebrate (CD) and neurologically intact controls. All rats were gavage fed an amount of diet that maintained weight gain in controls. CD rats were subjected to a two-stage surgery to produce a complete transection of the neuroaxis at the mesodiencephalic juncture.One week later, the rats were housed in an indirect calorimeter, and 24 h energy expenditure was measured for 4 d.One half of each of the CD and control groups was then starved for 48 h. Fed CD rats maintained a lower body temperature (35 C), a similar energy expenditure per unit fat-free mass but an elevated respiratory quotient compared with controls. They gained less weight, had 20% less lean tissue, and had 60% more fat than controls. Circulating leptin, adiponectin, and insulin were elevated, glucose was normal, but testosterone was dramatically reduced. Responses to starvation were similar in CD and controls; they reduced energy expenditure, decreased respiratory quotient, indicating lipid utilization, defended body temperature, mobilized fat, decreased serum leptin and insulin, and regulated plasma glucose. These data clearly demonstrate that the isolated caudal brainstem is sufficient to mediate many aspects of the energetic response to starvation. In intact animals, these responses may be refined by a contribution by more rostral brain areas or by communication between fore- and hind-brain. In the absence of communication from the fore-brain, the caudal brainstem is inadequate for maintenance of testosterone levels or lean tissue in fed or fasted animals.

UR - http://www.scopus.com/inward/record.url?scp=32644441245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32644441245&partnerID=8YFLogxK

U2 - 10.1210/en.2005-1156

DO - 10.1210/en.2005-1156

M3 - Article

C2 - 16357041

AN - SCOPUS:32644441245

VL - 147

SP - 1365

EP - 1376

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 3

ER -